A5014220124- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- Mycobacterium research and diagnosis
- Radiomics and Machine Learning in Medical Imaging
- Medical Imaging Techniques and Applications
- Inflammatory Bowel Disease
- Diagnosis and treatment of tuberculosis
- Health and Medical Research Impacts
- Cytokine Signaling Pathways and Interactions
- Advances in Oncology and Radiotherapy
- Glioma Diagnosis and Treatment
- Peptidase Inhibition and Analysis
University of Pittsburgh
2009-2018
University of Pittsburgh Medical Center
2015
Lung granulomas are the pathologic hallmark of tuberculosis (TB). T cells a major cellular component TB lung and known to play an important role in containment Mycobacterium (Mtb) infection. We used cynomolgus macaques, non-human primate model that recapitulates human with clinically active disease, latent infection or early infection, understand functional characteristics dynamics individual granulomas. sought correlate cell cytokine response bacterial burden each granuloma, as well...
ABSTRACT Cynomolgus macaques infected with low-dose Mycobacterium tuberculosis develop both active and latent infection similar to those of humans, providing an opportunity study the clinically silent early events in infection. 18 Fluorodeoxyglucose radiotracer positron emission tomography coregistered computed (FDG PET/CT) provides a noninvasive method measure disease progression. We sought determine temporal patterns granuloma evolution that distinguished active-disease outcomes. Macaques...
PET/CT imaging in macaques and humans with TB shows a beneficial therapeutic response to linezolid new oxazolidinone antibiotic, AZD5847.
Existing small-animal models of tuberculosis (TB) rarely develop cavitary disease, limiting their value for assessing the biology and dynamics this highly important feature human disease. To a smaller primate model with pathology similar to that seen in humans, we experimentally infected common marmoset (Callithrix jacchus) diverse strains Mycobacterium various pathogenic potentials. These included recent isolates modern Beijing lineage, Euro-American X M. africanum. All three produced...
Mycobacterium tuberculosis infection presents across a spectrum in humans, from latent to active tuberculosis. Among those with tuberculosis, it is now recognized that there also of and this likely contributes the variable risk reactivation Here, functional imaging 18F-fluorodeoxygluose positron emission tomography computed (PET CT) cynomolgus macaques M. was used characterize features after tumor necrosis factor (TNF) neutralization determine which characteristics before TNF distinguish...
Although recent studies in mice have shown that components of B cell and humoral immunity can modulate the immune responses against Mycobacterium tuberculosis, roles these human nonhuman primate infections are unknown. The cynomolgus macaque (Macaca fascicularis) model M. tuberculosis infection closely mirrors outcomes pathology (TB). present study used rituximab, an anti-CD20 antibody, to deplete cells tuberculosis-infected macaques examine contribution control TB primates during acute...
Mycobacterium tuberculosis remains the number one infectious agent in world today. With emergence of antibiotic resistant strains, new clinically relevant methods are needed that evaluate disease process and screen for potential vaccine treatments. Positron Emission Tomography/Computed Tomography (PET/CT) has been established as a valuable tool studying afflictions such cancer, Alzheimer's disease, inflammation/infection. Outlined here strategies have employed to PET/CT images cynomolgus...
Abstract Positron emission tomography and computed imaging (PET/CT) is an increasingly valuable tool for diagnosing tuberculosis (TB). The glucose analog [18F]fluoro-2-deoxy-2-d-glucose ([18F]-FDG) commonly used in PET/CT that retained by metabolically active inflammatory cells granulomas, but lacks specificity particular cell types. A PET probe could identify recruitment differentiation of different populations granulomas would be a useful research improve TB diagnosis treatment. We the...
Mycobacterium tuberculosis remains the number one infectious agent in world today. With emergence of antibiotic resistant strains, new clinically relevant methods are needed that evaluate disease process and screen for potential vaccine treatments. Positron Emission Tomography/Computed Tomography (PET/CT) has been established as a valuable tool studying afflictions such cancer, Alzheimer's disease, inflammation/infection. Outlined here strategies have employed to PET/CT images cynomolgus...
Little is known about granuloma progression of Mycobacterium tuberculosis infection in humans. Using serial positron emission tomography and computed (PET/CT) an animal model that recapitulates human with M. tuberculosis, we are able to track lung granulomas. We characterized the spatial temporal pattern formation during primary reactivation. Serial PET/CT was performed on cynomolgus macaques (n = 28) reactivation infection. Distances between granulomas first six weeks post ("primary"...