A5069816588- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Olfactory and Sensory Function Studies
- Biochemical Analysis and Sensing Techniques
- Neurobiology and Insect Physiology Research
- Fungal Biology and Applications
- Immune cells in cancer
- Mesenchymal stem cell research
- Advanced biosensing and bioanalysis techniques
- Plant Pathogenic Bacteria Studies
- Cellular Mechanics and Interactions
- Bacteriophages and microbial interactions
- Pneumocystis jirovecii pneumonia detection and treatment
- Retinal Development and Disorders
- Calpain Protease Function and Regulation
- Muscle Physiology and Disorders
- Nitric Oxide and Endothelin Effects
- Connexins and lens biology
- S100 Proteins and Annexins
- Genomics and Phylogenetic Studies
- Cell Adhesion Molecules Research
The Wallace H. Coulter Department of Biomedical Engineering
2021-2023
Georgia Institute of Technology
2021-2023
University of California, San Diego
2019
Texas A&M University
2019
Western University of Health Sciences
2016
Yale University
2011
University of Florida
2007-2009
Florida College
2007
Duke Medical Center
2006
Olfactory sensory neurons converge onto glomeruli in the olfactory bulb (OB) to form modular information processing units. Similar input modules are organized translaminar columns for other modalities. It has been less clear OB whether initial organization relates a columnar structure deeper layers involved local circuit processing. To probe synaptic connectivity OB, we injected retrograde-specific strain of pseudorabies virus into rat and piriform cortex. The viral-staining patterns...
The use of biochemical signaling to derive smooth muscle cells (SMCs) from mesenchymal stem (MSCs) has been explored, but the induction a fully functional SMC phenotype remains be major challenge. Cell morphology shown regulate MSC differentiation into various lineages, including SMCs. We engineered substrates with microgrooves induce cell elongation study mechanism underlying shape modulation in differentiation. In comparison those on flat substrates, MSCs cultured were elongated increased...
We showed that a Candida albicans petite mutant in which oxidative phosphorylation is uncoupled was eightfold more resistant to fluconazole and voriconazole than SC5314 but equally susceptible ketoconazole, itraconazole, amphotericin B. Strain P5 significantly overexpressed MDR1, likely accounts for the decreased drug susceptibility.
Lateral connections in the olfactory bulb were previously thought to be organized for center-surround inhibition. However, recent anatomical and physiological studies showed sparse distributed interactions of inhibitory granule cells (GCs) which tended columnar clusters. Little is known about how these clusters are interconnected. In this study, we use transsynaptic tracing viruses bearing green or red fluorescent proteins further elucidate mitral- tufted-to-GC connectivity. Separate sites...
Deciphering the complex interplay of neutrophil extracellular traps (NETs) with surrounding environment is a challenge notable clinical implications. To bridge gap in knowledge, we report our findings on antibacterial activity against Pseudomonas aeruginosa synthetic NET-mimetic materials composed nanofibrillated DNA-protein complexes. Our system makes component-by-component bottom-up analysis NET protein effects possible. When antimicrobial enzyme elastase (NE) incorporated into...
Anidulafungin targets the cell walls of Candida species by inhibiting beta-1,3-glucan synthase, thereby killing isolates and exerting prolonged postantifungal effects (PAFEs). We performed time-kill PAFE experiments on albicans (n = 4), C. glabrata 3), parapsilosis krusei 2) characterized PAFEs in greater detail. MICs were 0.008 to 0.125 microg/ml against albicans, glabrata, 1.0 2.0 parapsilosis. During experiments, anidulafungin caused significant kills at 16x MIC (range, log 2.68 3.89) 4x...
Neutrophil extracellular traps (NETs) is an antimicrobial cobweb-structured material produced by immune cells for clearance of pathogens in the body, but paradoxically associated with biofilm formation and exacerbated lung infections. To provide a better materials perspective on pleiotropic roles played NETs at diverse compositions/concentrations, NETs-like (called 'microwebs', abbreviated as μwebs) synthesized decoding activity against Staphylococcus aureus infection-relevant conditions. We...
May is a newly isolated myophage that infects multidrug-resistant strains of Klebsiella pneumoniae, pathogen associated with antibiotic-resistant infections in humans. The genome has been shown to be similar phage Vi01.
This study analyzed the nuclease- and serum-driven degradation of millimeter-scale, circular DNA-histone mesostructures (DHMs). DHMs are bioengineered chromatin meshes defined DNA histone compositions designed as minimal mimetics physiological extracellular structures, such neutrophil traps (NETs). Taking advantage shape DHMs, an automated time-lapse imaging image analysis method was developed used to track DHM changes over time. were degraded well by 10 U/mL concentrations deoxyribonuclease...