A5040880742- Hematopoietic Stem Cell Transplantation
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Cellular Mechanics and Interactions
- Developmental Biology and Gene Regulation
- Acute Myeloid Leukemia Research
- Acute Lymphoblastic Leukemia research
- Genetics, Aging, and Longevity in Model Organisms
- Hearing, Cochlea, Tinnitus, Genetics
- Childhood Cancer Survivors' Quality of Life
- Congenital heart defects research
- Neuroblastoma Research and Treatments
- Bangladesh Politics, Society, and Development
- T-cell and B-cell Immunology
- Zebrafish Biomedical Research Applications
- Cytomegalovirus and herpesvirus research
- Genetic Mapping and Diversity in Plants and Animals
- Bone Tissue Engineering Materials
- Advanced Materials and Mechanics
- Hemoglobinopathies and Related Disorders
- Mesenchymal stem cell research
- Marine Biology and Environmental Chemistry
- 3D Printing in Biomedical Research
- Politics and Conflicts in Afghanistan, Pakistan, and Middle East
- Digital Games and Media
Nkarta Therapeutics (United States)
2021-2024
University of Virginia
2007-2024
AdventHealth for Children
2018-2020
St. Jude Children's Research Hospital
2009-2020
AdventHealth Orlando
2019
University of Virginia Health System
2018
University of Tennessee Health Science Center
2010-2017
Merrill (United States)
2015
University of Oregon
2014
Stony Brook University
2012
To develop new therapies for children with solid tumors, we tested the cytotoxicity of natural killer (NK) cells expanded by coculture K562-mb15-41BBL cells. We sought to identify most sensitive tumor subtypes, clarify molecular interactions regulating cytotoxicity, and determine NK antitumor potential in vivo.We vitro against cell lines representative Ewing sarcoma (EWS; n = 5), rhabdomyosarcoma (n 4), neuroblastoma 3), osteosarcoma correlated results expression inhibitory activating...
We discuss the physical aspects of morphogenic process convergence (narrowing) and extension (lengthening) tissues by cell intercalation. These movements, often referred to as 'convergent extension', occur in both epithelial mesenchymal during embryogenesis organogenesis invertebrates vertebrates, they play large roles shaping body plan development. Our focus is on presumptive mesodermal neural Xenopus (frog) embryo, for which some measurements have been made. how polarized motility,...
HLA-matched related donor (MRD) hematopoietic stem cell transplantation (HSCT) is a well-established therapy for patients with sickle disease (SCD); however, experience using alternative donors, including haploidentical in HSCT SCD limited. We report the long-term outcomes of 22 pediatric who underwent at St. Jude Children's Research Hospital, either myeloablative sibling MRD (n = 14) or reduced-intensity parental 8). The median patient age was 11.0 ± 3.9 years graft recipients and 9.0 5.0...
Vertebrate embryos undergo dramatic shape changes at gastrulation that require locally produced and anisotropically applied forces, yet how these forces are transmitted across tissues remains unclear. We show depletion of myosin regulatory light chain (RLC) levels in the embryo blocks force generation through two distinct mechanisms: destabilizing II (MII) hexameric complex inhibiting MII contractility. Molecular dissection mechanisms demonstrates normal convergence requires contractility we...
Indirect evidence suggests that blastopore closure during gastrulation of anamniotes, including amphibians such as Xenopus laevis, depends on circumblastoporal convergence forces generated by the marginal zone (MZ), but direct is lacking. We show explanted MZs generate tensile up to 1.5 μN and over 4 thereafter. These are convergent thickening (CT) until midgastrula increasingly extension (CE) Explants from ventralized embryos, which lack tissues expressing CE close their blastopores,...
Abstract We have identified, using composite interval mapping, quantitative trait loci (QTL) affecting a variety of life history traits (LHTs) in the nematode Caenorhabditis elegans. Using recombinant inbred strains assayed on surface agar plates, we found QTL for survival, early fertility, age onset sexual maturity, and population growth rate. There was no overall correlation between survival solid media previous measures liquid media. Of four these two environments, genotype-environment...
To measure longitudinal changes in body mass and composition survivors of childhood hematologic malignancies after allogeneic hematopoietic stem-cell transplantation (HSCT).Body index (BMI) was analyzed 179 by category (underweight, healthy-weight, overweight, obese) z score. Fat lean measured dual-energy x-ray absorptiometry as scores.Over a median 6.6 years follow-up, BMI scores diminished significantly (0.32 pre-HSCT v -0.60 at 10 post-HSCT; P < .001). Mean for fat stayed within...
Abstract Background T‐cell depletion ( TCD ) effectively reduces severe graft‐versus‐host disease in recipients of HLA ‐mismatched allografts. However, is associated with delayed immune recovery and increased infections. We hypothesized that specific CD 45 RA + naive T cells, rather than broad 3+ can preserve memory‐immunity the allografts confer protection against important viral infections early post‐transplant period. Methods Sixty‐seven patients who received haploidentical donor...
Background Haploidentical donors are being increasingly used for allogeneic hematopoietic cell transplantation (HCT). However, the requisite T-cell depletion results in a profound and often long-lasting immunocompromised state, donor lymphocyte infusions bring risk of graft-versus-host disease (GVHD). Naïve T-cells believed to be among most alloreactive subset can identified by CD45RA expression. Allogeneic HCT using has not been previously described haploidentical donors. Procedure Eight...
Abstract Introduction Advances in autologous hematopoietic stem cell transplantation ( HSCT ) over the past 20 years may have had an impact on morbidity and mortality associated with infections post transplant. Patients methods We sought to retrospectively analyze epidemiology of first episode bacterial, fungal, viral, or parasitic 0–30 days transplant a cohort 320 children adolescents who underwent single institution, between 1990 2009 for solid tumors lymphoma, 65 transplanted acute...
The treatment of pediatric high-risk neuroblastoma is intensive and multimodal. Despite the introduction immunotherapy for minimal residual disease, survival rates remain suboptimal new therapies are needed. As part a phase 2 trial, we using consolidation therapy regimen that combines busulfan/melphalan conditioning schema, autologous hematopoietic cell transplantation (AHCT), experimental with hu14.18K322A (a humanized anti-GD2 monoclonal antibody), granulocyte-macrophage colony-stimulating...
Background Novel therapies are needed for children with relapsed or refractory leukemia. We therefore tested the safety and feasibility of haploidentical natural killer cell therapy in this patient population. Procedure Twenty‐nine who had leukemia were treated chemotherapy followed by infusion NK cells. Cohort 1 included 14 not undergone prior allogeneic hematopoietic transplantation (HCT), whereas 2 15 that after HCT. Results Twenty‐six (90%) donors KIR mismatched (14 one 12 KIRs). The...
Abstract BACKGROUND The objectives of the current study were to: 1) characterize clinical outcome patients with recurrent micropapillary serous ovarian carcinoma (MPSC) and 2) evaluate survival impact secondary cytoreductive surgery other prognostic variables. METHODS Twenty‐six MPSC identified retrospectively from hospital tumor registry databases. Survival curves generated time recurrence using Kaplan–Meier method statistical comparisons performed log‐rank test, logistic regression...
We have identified chromosomal regions containing quantitative trait loci (QTLs) specifying life history traits in recombinant-inbred strains of the nematode Caenorhabditis elegans. This approach also allows us to examine epistatic interactions between and pleiotropic effects on different at specific loci. QTLs for mean span were chromosomes II (near stP101), IV (stP5) X (stP61), fertility (maPI), III (stP19) (stP51). The accounted 90% genetic component variance. 88% Additional...
To study the role of keratin filaments in Xenopus development, fertilized eggs were injected with anti-keratin monoclonal antibodies. The antibodies AE1 and AE3 induce abnormal gastrulation; most severely affected embryos gastrulation fails completely. In contrast, antibody 1h5 develop normally. Immunocytochemical data indicate that binds to dense superficial filament system embryo but not deeper networks ectodermal subectodermal cells. Injected do bind appear interact preferentially deep...