A5000241286- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- RNA Research and Splicing
- Viral Infections and Immunology Research
- interferon and immune responses
- Microbial Community Ecology and Physiology
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- RNA regulation and disease
- Cytokine Signaling Pathways and Interactions
- Plant and Fungal Interactions Research
- Zebrafish Biomedical Research Applications
- RNA and protein synthesis mechanisms
- Tailings Management and Properties
- Antimicrobial agents and applications
- Neuroendocrine regulation and behavior
- Respiratory viral infections research
- Nanoplatforms for cancer theranostics
- Animal Virus Infections Studies
- Coal Combustion and Slurry Processing
- Influenza Virus Research Studies
- Photodynamic Therapy Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Marine Ecology and Invasive Species
- Virus-based gene therapy research
University of British Columbia
2018-2024
North-West University
2020
Rhodes University
2015
The upside-down jellyfish Cassiopea xamachana (Scyphozoa: Rhizostomeae) has been predominantly studied to understand its interaction with the endosymbiotic dinoflagellate algae Symbiodinium. As an easily culturable and tractable cnidarian model, it is attractive alternative stony corals understanding mechanisms driving establishment maintenance of symbiosis. also unique in requiring symbiont order complete transition adult stage, thereby providing excellent model symbiosis-driven development...
The main viral protease (3CL
RNA viruses, particularly genetically diverse members of the Picornavirales, are widespread and abundant in ocean. Gene surveys suggest that there spatial temporal patterns composition virus assemblages, but data on their diversity genetic variability different oceanographic settings limited. Here, we show specific genomes have geographic distributions dominant genotypes under purifying selection. Genomes from three previously unknown picorna-like viruses (BC-1, -2, -3) assembled a coastal...
The development of low-cost, non-toxic, scalable antimicrobial textiles is needed to address the spread deadly pathogens. Here, we report a polysiloxane textile coating that possesses two modes inactivation, passive contact inactivation through amine/imine functionalities and active photodynamic generation reactive oxygen species (ROS). This material can be coated cross-linked onto natural synthetic simple soak procedure, followed by UV cure afford materials exhibiting no aqueous leaching...
The family Marnaviridae comprises small non-enveloped viruses with positive-sense RNA genomes of 8.6-9.6 kb. Isolates infect marine single-celled eukaryotes (protists) that come from diverse lineages. Some members are known metagenomic studies ocean virioplankton, additional unclassified described datasets derived and freshwater environments. This is a summary the International Committee on Taxonomy Viruses (ICTV) Report Marnaviridae, which available at ictv.global/report/marnaviridae.
Abstract Metagenomics has altered our understanding of microbial diversity and ecology. This includes its applications to viruses in marine environments that have demonstrated their enormous diversity. Within these are RNA viruses, many which share genetic features with members the order Picornavirales; yet, very few been taxonomically classified. The only recognized family is Marnaviridae, was founded based on discovery characterization species Heterosigma akashiwo virus. Two additional...
Viruses have evolved diverse strategies to evade the host innate immune response and promote infection. The retinoic acid-inducible gene I (RIG-I)-like receptors RIG-I MDA5 are antiviral factors that sense viral RNA trigger downstream signal via mitochondrial antiviral-signaling protein (MAVS) activate type interferon expression. 14-3-3ε is a key component of translocon complex interacts with MAVS at membrane; however, exact role in this pathway not well understood. In study, we demonstrate...
Charophyte algae, not chlorophyte are the ancestors of ‘higher plants’; hence, viruses infecting charophytes may be related to those that first infected higher plants. Streamwaters from British Columbia, Canada, yielded single-stranded RNA metagenomes Charavirus canadensis (CV-Can), similar in genomic architecture, length (9593 nt), nucleotide identity (63.4%), and encoded amino-acid sequence (53.0%) australis (CV-Aus). The sequences their RNA-dependent RNA-polymerases (RdRp) resemble found...
Myocarditis, inflammation of the heart muscle, affects all demographics and is a major cause sudden unexpected death in young people. It most commonly caused by viral infections heart, with Coxsackievirus B3 (CVB3) being among prevalent pathogens. To understand molecular pathogenesis CVB3 infection provide strategies for developing treatments, we examined role key nuclear pore protein 98 (NUP98) setting myocarditis. NUP98 was cleaved as early 2 h post-CVB3 infection. This cleavage further...
Abstract Freshwater is an essential resource of increasing value, as clean water sources diminish. Microorganisms in rivers, a major source renewable freshwater, are significant due to their role drinking safety, signalling environmental contamination 1 , and driving global nutrient cycles 2,3 . However, foundational understanding microbial communities rivers lacking 4 especially temporally for viruses 5‒7 No studies date have examined the composition free-floating river virome over time,...
ABSTRACT Coxsackievirus B3 (CVB3) encodes proteinases that are essential for processing of the translated viral polyprotein. Viral also target host proteins to manipulate cellular processes and evade innate antiviral responses promote replication infection. While some protein substrates CVB3 3C 2A cysteine have been identified, full repertoire targets is not known. Here, we utilize an unbiased quantitative proteomics-based approach termed terminal amine isotopic labeling (TAILS) conduct a...
Viruses have evolved mechanisms to modulate cellular pathways facilitate infection. One such pathway is the formation of stress granules (SG), which are ribonucleoprotein complexes that assemble during translation inhibition following stress. Inhibition SG assembly has been observed under numerous virus infections across species, suggesting a conserved fundamental viral strategy. However, significance modulation infection not fully understood. The 1A protein encoded by model dicistrovirus,...
Abstract To identify and characterize roles for SARS-CoV-2 3CL pro main protease in promoting viral infection dissemination, we employed Inactive Catalytic Domain Capture proteomics to host cell interactors substrates the interactome of . Of 259 interactors, 145 were associated with cytoskeleton its organisation. We determined enzyme kinetic specificity constants 139 cut-sites 43 using a multiplex assay, identifying 29 efficiently-cleaved validating 13 as vitro , SARS-CoV-2-infected human...
The dicistrovirus intergenic region internal ribosome entry site (IGR IRES) uses an unprecedented, streamlined mechanism whereby the IRES adopts a triple-pseudoknot (PK) structure to directly bind conserved core of and drive translation from non-AUG codon. origin this is not known. Previously, partial fragment divergent RNA genome, named ancient Northwest territories cripavirus (aNCV), was extracted 700-year-old caribou feces trapped in subarctic ice patch. aNCV IGR sequence secondary...
The Tronox KZN Sands Hillendale Mine is located in Zululand -close to Richards Bay.Mining activities commenced 2001 and the mine's production came an end 2013.At Hillendale, dunes with a high fines content were mined recover valuable minerals of ilmenite Zircon rutile.The fine fraction consisting mainly clay was managed by storage residue facility (RSF).Following cessation mining several actions have been implemented towards achieving mine closure.This paper will firstly give some basic...
ABSTRACT Viruses have evolved mechanisms to modulate cellular pathways facilitate infection. One such pathway is the formation of stress granules (SG), which are ribonucleoprotein complexes that assemble during translation inhibition following stress. Inhibition SG assembly has been observed under numerous virus infections across species, suggesting a conserved fundamental viral strategy. However, significance modulation infection not fully understood. The 1A protein encoded by model...
Abstract Viruses have evolved diverse strategies to evade the host innate immune response and promote infection. The RIG-I-like-receptors RIG-I MDA5 (RLRs) are antiviral factors that sense viral RNA signal downstream via mitochondrial antiviral-signaling protein (MAVS) activate type I interferon (IFN) expression. 14-3-3ε is a key component of translocon complex interacts with MAVS at membrane; however, exact role in this pathway not well understood. In study, we demonstrate direct substrate...