A5086895840- Coral and Marine Ecosystems Studies
- Aquaculture disease management and microbiota
- Marine Sponges and Natural Products
- Marine and coastal plant biology
- Marine and fisheries research
- Environmental Toxicology and Ecotoxicology
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Ocean Acidification Effects and Responses
- Ichthyology and Marine Biology
- Identification and Quantification in Food
- Skin Protection and Aging
- Pharmaceutical and Antibiotic Environmental Impacts
- Coastal and Marine Management
- Plant Pathogens and Fungal Diseases
- Viral gastroenteritis research and epidemiology
- Microplastics and Plastic Pollution
- Apelin-related biomedical research
- Genomics and Phylogenetic Studies
- Physiological and biochemical adaptations
- Marine Invertebrate Physiology and Ecology
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Hepatitis Viruses Studies and Epidemiology
- Coastal wetland ecosystem dynamics
- Microbial Community Ecology and Physiology
NCCOS Hollings Marine Laboratory
2013-2024
National Oceanic and Atmospheric Administration
2014-2024
NOAA National Centers for Coastal Ocean Science
2003-2024
NOAA National Ocean Service
2013-2024
St Thomas' Hospital
2023
Medical University of South Carolina
1983-2006
Baylor College of Medicine
1994-1996
Centers for Disease Control and Prevention
1993
Contemporary in-depth sequencing of environmental samples has provided novel insights into microbial community structures, revealing that their diversity had been previously underestimated. Communities in marine environments are commonly composed a few dominant taxa and high number taxonomically diverse, low-abundance organisms. However, studying the roles genomic information these "rare" organisms remains challenging, because little is known about ecological niches conditions to which they...
A method for the detection of Norwalk virus and hepatitis from shellfish tissues by PCR was developed. Virus added to stomach hepatopancreatic oysters or hard-shell clams, viral nucleic acids were purified a modification previously described (R.L. Atmar, T.G. Metcalf, F.H. Neill, M.K. Estes, Appl. Environ. Microbiol. 59:631-635, 1993). The new had following advantages compared with method: (i) more rapid sample processing; (ii) increased test sensitivity; (iii) decreased sample-associated...
The upside-down jellyfish Cassiopea xamachana (Scyphozoa: Rhizostomeae) has been predominantly studied to understand its interaction with the endosymbiotic dinoflagellate algae Symbiodinium. As an easily culturable and tractable cnidarian model, it is attractive alternative stony corals understanding mechanisms driving establishment maintenance of symbiosis. also unique in requiring symbiont order complete transition adult stage, thereby providing excellent model symbiosis-driven development...
Coral bleaching is a significant contributor to the worldwide degradation of coral reefs and indicative termination symbiosis between host its symbiotic algae (dinoflagellate; Symbiodinium sp. complex), usually by expulsion or xenophagy (symbiophagy) dinoflagellates. Herein, we provide evidence that during earliest stages environmentally induced bleaching, heat stress light generate distinctly different pathomorphological changes in chloroplasts, while combined heat- light-stress exposure...
A monoclonal antibody against purified rat urinary kallikrein was coupled to agarose and used isolate from brain. The enzyme has N alpha-tosyl-L-arginine methyl esterase activity with a pH optimum at 9.0, kinin-releasing low molecular weight kininogen, parallelism standard curves of in direct radioimmunoassay. Brain is inhibited by series tissue inhibitors IC50 values similar those for kallikrein. brain labeled [14C]diisopropylphosphorofluoridate visualized fluorography on sodium dodecyl...
AbstractCoral bleaching is a major contributor to the global declines of coral reefs. This phenomenon characterized by loss symbiotic algae, their pigments or both. Despite wide scientific interest, mechanisms which occurs still poorly understood. Here we report that removal symbiont during light and temperature stress achieved using host's cellular autophagic-associated machinery. Host sub-cellular morphologies showed increased vacuolization appearance autophagic membranes surrounding...
A cooperative study was conducted by the International Working Group on Mycobacterial Taxonomy to correlate agglutination serovar designations of Mycobacterium avium, intracellulare, and scrofulaceum strains with species ascriptions these organisms according molecular criteria cultural properties assess reproducibility determinations for a set 63 reference species. Among criteria, level agreement between results obtained nucleic acid probes T-catalase serology 94% M. avium intracellulare....
An in situ transcription method was developed to detect hepatitis A virus RNA both cell cultures and shellfish tissues. Radiolabeled cDNA copies were synthesized by reverse transcriptase-directed after annealing with a specific primer the viral RNA. Both tritium (3H) 35S useful reaction, but use of 3H resulted lower background finer detail localization particles. Application different organs oysters which had bioaccumulated allowed first virus, specifically stomach hepatopancreatic
A multicenter, collaborative trial was performed to evaluate the reliability and reproducibility of a previously described method for detection Norwalk virus in shellfish tissues with PCR (R.L. Atmar, F. H. Neill, J. L. Romalde, Le Guyader, C. M. Woodley, T. G. Metcalf, K. Estes, Appl. Environ. Microbiol. 61:3014-3018, 1995). Virus added stomachs hepatopancreatic oysters or hard-shell clams control laboratory, samples were shipped participating laboratories, viral nucleic acids extracted...
A specific monoclonal antibody against rat tissue kallikrein was used as the primary for indirect immunoperoxidase staining of hypothalamus. Kallikrein localized in epithelial cells (ependyma) lining third ventricle well cell bodies arcuate, supraoptic, paraventricular, and ventromedial nuclei.