A5070893142- Protein Structure and Dynamics
- Enzyme Structure and Function
- Receptor Mechanisms and Signaling
- Bacterial Genetics and Biotechnology
- Ubiquitin and proteasome pathways
- Heat shock proteins research
- Mass Spectrometry Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Neuropeptides and Animal Physiology
- Computational Drug Discovery Methods
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Tea Polyphenols and Effects
- Glycosylation and Glycoproteins Research
- Plant Molecular Biology Research
- RNA and protein synthesis mechanisms
- Phytochemicals and Antioxidant Activities
- Plant Pathogens and Fungal Diseases
- Autophagy in Disease and Therapy
- Congenital Heart Disease Studies
- Plant and animal studies
- Crystallization and Solubility Studies
- Plant and Fungal Species Descriptions
- Plant nutrient uptake and metabolism
- X-ray Diffraction in Crystallography
Wuhan Institute of Physics and Mathematics
2019-2025
Chinese Academy of Sciences
2019-2025
Fujian Agriculture and Forestry University
2024-2025
Changzhou Vocational Institute of Light Industry
2025
Yunnan Agricultural University
2025
Tianjin University of Traditional Chinese Medicine
2023-2024
University of Chinese Academy of Sciences
2019-2024
Lanzhou University
2023-2024
Vanderbilt University
2023-2024
Wuhan Ship Development & Design Institute
2024
Pattern recognition receptors confer plant resistance to pathogen infection by recognizing the conserved pathogen-associated molecular patterns. The cell surface receptor chitin elicitor kinase 1 of Arabidopsis (AtCERK1) directly binds through its lysine motif (LysM)-containing ectodomain (AtCERK1-ECD) activate immune responses. crystal structure that we solved an AtCERK1-ECD complexed with a pentamer reveals their interaction is primarily mediated LysM and three residues. By acting as...
Significance Recent structures of GPCRs in complex with G proteins provide important insights into protein activation by family A and B GPCRs; however, questions remain. We don’t fully understand the mechanism coupling specificity or promiscuity some GPCRs. The β 2 AR preferentially couples to s less efficiently i , yet AR-G has been shown play roles cardiac physiology. To better structural basis for preferential over we used NMR spectroscopy supporting MD simulations study conformational...
Abstract Advances in structural biology have provided important mechanistic insights into signaling by the transmembrane core of G-protein coupled receptors (GPCRs); however, much less is known about intrinsically disordered regions such as carboxyl terminus (CT), which highly flexible and not visible GPCR structures. The β 2 adrenergic receptor’s (β AR) 71 amino acid CT a substrate for kinases binds β-arrestins to regulate signaling. Here we show that AR directly inhibits basal...
Abstract The M2 muscarinic receptor (M2R) is a prototypical G-protein-coupled (GPCR) that serves as model system for understanding GPCR regulation by both orthosteric and allosteric ligands. Here, we investigate the mechanisms governing M2R signaling versatility using cryo-electron microscopy (cryo-EM) NMR spectroscopy, focusing on physiological agonist acetylcholine supra-physiological iperoxo, well positive modulator LY2119620. These studies reveal stabilizes more heterogeneous...
Spatial transcriptomics (ST) is advancing our understanding of complex tissues and organisms. However, building a robust clustering algorithm to define spatially coherent regions in single tissue slice aligning or integrating multiple slices originating from diverse sources for essential downstream analyses remains challenging. Numerous clustering, alignment, integration methods have been specifically designed ST data by leveraging its spatial information. The absence comprehensive benchmark...
The conversion of prion protein (PrP) to the pathogenic PrPSc conformation is central disease. Previous studies revealed that PrP interacts with lipids and interaction induces conformational changes, yet it remains unclear whether in absence any denaturing treatment, PrP−lipid sufficient convert classic proteinase K-resistant conformation. Using recombinant mouse PrP, we analyzed under physiological conditions followed lipid-induced change K (PK) digestion. We found was initiated by...
The twin-arginine transport (Tat) system translocates folded proteins across the bacterial cytoplasmic or chloroplast thylakoid membrane of plants. Tat in most Gram-positive bacteria consists two essential components, TatA and TatC proteins. is considered to be a bifunctional subunit, which can form protein-conducting channel by self-oligomerization also participate substrate recognition. However, molecular mechanism underlying protein translocation remains elusive. Herein, we report...
Plants employ various molecular mechanisms to maintain primary root elongation upon salt stress. Identification of key functional genes, therein, is important for improving crop tolerance. Through analyzing natural variation the length Arabidopsis population under stress, we identified NIGT1.4, encoding an MYB transcription factor, as a novel contributor maintained growth Using both T-DNA knockout and complementation, NIGT1.4 was confirmed have role in promoting response The expression shown...
With the continuous expansion of organic tea industry, distinguishing authenticity is crucial to maintain market's stability. Thus, and conventional planting teas (green teas) Dajianshan were selected as objects in this study. The components (water extract, polyphenols, mineral element, etc.) compared by high-performance liquid chromatography, inductively coupled plasma spectrometry, mass spectrometry. main difference substances screened multivariate statistical analysis methods (PCA,...
Despite the growing number of G protein-coupled receptor (GPCR) structures being resolved, dynamic process how GPCRs transit from inactive toward active state remains unclear. In this study, comprehensive molecular dynamics simulations were performed to explore ligand binding modulates conformational M2 muscarinic acetylcholine (M2R). We observed a sequential occurrence structural changes in inactive-to-active transition M2R induced by superagonist iperoxo, which includes orthosteric site...
Gallic acid (GA), as a precursor of Epigallocatechin-3-gallate (EGCG) biosynthesis in tea plant, is one the important components flavor and has various health benefits. However, mechanism endogenous hormones regulating GA during development buds leaves shoots still unclear. In this study, five different developmental stages were used test materials to explore hormone signaling pathway biosynthesis. The results showed that decrease D-erythrosyl-4-phosphate content increase shikimic affected...
A regioselective coupling of aliphatic ketones with alkenes has been realized by cathodic reduction. This reaction enables the formation ketyl radicals and activation challenging under mild electrolysis conditions, providing an effective protocol for accessing diverse tertiary alcohols substrate-dependent regioselectivity. The practicability this is demonstrated scale-up experiments. hydrogen source products, migration isomerization allylarenes, applicability internal are control
Advancements in spatial transcriptomics (ST) have enabled an in-depth understanding of complex tissues by quantifying gene expression at spatially localized spots. Several notable clustering methods been introduced to utilize both and transcriptional information the analysis ST datasets. However, data quality across different sequencing techniques types datasets influence performance benchmarks. To harness context profile data, we developed a graph-based, multi-stage framework for robust...
Arsenic compounds commonly exist in nature and are toxic to nearly all kinds of life forms, which directed the evolution enzymes many organisms for arsenic detoxification. In bacteria, thioredoxin-coupled arsenate reductase catalyzes reduction arsenite by intramolecular thiol-disulfide cascade. The oxidized ArsC is subsequently regenerated thioredoxin through an intermolecular exchange process. solution structure Bacillus subtilis thioredoxin-arsenate complex represents transiently formed...