A5087186394- Receptor Mechanisms and Signaling
- Drug Transport and Resistance Mechanisms
- DNA and Nucleic Acid Chemistry
- Neuropeptides and Animal Physiology
- Lipid Membrane Structure and Behavior
- Cancer therapeutics and mechanisms
- Chemical Synthesis and Analysis
- Protein Structure and Dynamics
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Anomaly Detection Techniques and Applications
- Problem and Project Based Learning
- Explainable Artificial Intelligence (XAI)
- Pharmacological Effects and Assays
- Digital Media and Visual Art
- Amino Acid Enzymes and Metabolism
- Protein Kinase Regulation and GTPase Signaling
- Engineering Education and Curriculum Development
- Molecular Sensors and Ion Detection
- Force Microscopy Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Coordination Chemistry and Organometallics
- IoT and Edge/Fog Computing
- Bacteriophages and microbial interactions
- Human-Automation Interaction and Safety
University of Newcastle Australia
2024-2025
Beijing Advanced Sciences and Innovation Center
2020-2024
Tsinghua University
2020-2024
Center for Life Sciences
2021-2024
King Center
2023
Jingdong (China)
2023
Mianyang Normal University
2023
Chinese Academy of Sciences
2011-2017
Institute of Biophysics
2011-2017
University of Chinese Academy of Sciences
2015
Abstract The µ-opioid receptor (µOR) is an important target for pain management 1 and molecular understanding of drug action on µOR will facilitate the development better therapeutics. Here we show, using double electron–electron resonance single-molecule fluorescence energy transfer, how ligand-specific conformational changes translate into a broad range intrinsic efficacies at transducer level. We identify several conformations cytoplasmic face that interconvert different timescales,...
The major facilitator superfamily (MFS) is the largest family of secondary active transporters and present in all life kingdoms. Detailed structural basis substrate transport energy-coupling mechanisms these proteins remain to be elucidated. YajR a putative proton-driven MFS transporter found many Gram-negative bacteria. Here we report crystal structure Escherichia coli at 3.15 Å resolution an outward-facing conformation. In addition having 12 canonical transmembrane helices, includes unique...
Significance Recent structures of GPCRs in complex with G proteins provide important insights into protein activation by family A and B GPCRs; however, questions remain. We don’t fully understand the mechanism coupling specificity or promiscuity some GPCRs. The β 2 AR preferentially couples to s less efficiently i , yet AR-G has been shown play roles cardiac physiology. To better structural basis for preferential over we used NMR spectroscopy supporting MD simulations study conformational...
Abstract Advances in structural biology have provided important mechanistic insights into signaling by the transmembrane core of G-protein coupled receptors (GPCRs); however, much less is known about intrinsically disordered regions such as carboxyl terminus (CT), which highly flexible and not visible GPCR structures. The β 2 adrenergic receptor’s (β AR) 71 amino acid CT a substrate for kinases binds β-arrestins to regulate signaling. Here we show that AR directly inhibits basal...
Hazard detection in industrial and public environments is critical for ensuring safety regulatory compliance. This paper presents an energy-efficient, cost-effective IoT-based hazard system utilizing ESP32-CAM microcontroller integrated with temperature (DHT22) motion (PIR) sensors. A custom-built convolutional neural network (CNN) deployed on a Flask server enabled real-time classification of signs, including “high voltage”, “radioactive”, “corrosive”, “flammable”, “no hazard”, smoking”,...
The β2-adrenergic receptor (β2AR) is a G-protein-coupled (GPCR) that responds to the hormone adrenaline and an important drug target in context of respiratory diseases, including asthma. β2AR function can be regulated by post-translational modifications such as phosphorylation ubiquitination at C-terminus, but access full-length with well-defined homogeneous modification patterns critical for biochemical biophysical studies remains challenging. Here, we report practical synthesis...
In traditional classroom settings, monitoring students’ attention and engagement levels remains a persistent challenge. This paper presents an action research study that integrates real-time learning analytics using video wearable sensors to support instructional interventions enhance experiences. The system captures group-level activity data through skeleton-based processing smartwatches, analyzing trends without storing personal recordings. It provides instructors with instant feedback via...
Abstract Advances in singe-particle cryo-electron microscopy (cryo-EM) have made possible to solve the structures of numerous Family A and B G protein coupled receptors (GPCRs) complex with proteins arrestins, as well several C GPCRs. Determination these has been facilitated by presence large extra-membrane components (such protein, arrestin, or Venus flytrap domains) complexes that aid particle alignment during processing cryo-EM data. In contrast, determination inactive state structure...
Abstract The μ-opioid receptor (μOR) is an important target for pain management and the molecular understanding of drug action will facilitate development better therapeutics. Here we show, using double electron-electron resonance (DEER) single-molecule fluorescence energy transfer (smFRET), how ligand-specific conformational changes μOR translate into a broad range intrinsic efficacies at transducer level. We identify several cytoplasmic conformations interconverting on different...
The DHA12 family of transporters contains a number prokaryotic and eukaryote membrane proteins. Some these proteins share conserved sites intrinsic to substrate recognition, structural stabilization conformational changes. For this study, we chose the MdfA transporter as model protein study some general characteristics vesicular neurotransmitter (VNTs), which all belong family. Two crystal structures were produced for E. coli MdfA, one in complex with acetylcholine other potential reserpine,...
Abstract DNA double-strand breaks (DSBs) are highly toxic lesions that occur during the cellular metabolic process. Polymerase theta (Polθ) is an error-prone polymerase has been implicated in repair of chromosome breaks, recovery broken replication forks, and translesion synthesis. The inhibition Polθ activity killing HR-deficient tumor cells vitro vivo . We present first biochemical evidence antibiotics novobiocin (NVB) noncompetitively inhibit ATP hydrolysis by ATPase domain helicase...
Abstract Although there has been enormous progress in the last half-century drug discovery targeting obesity and associated co-morbidities, clinical treatment of remains tremendously challenging. GPR75 is an orphan receptor suggested to be a potential novel target for control related metabolic disorders. Inhibition signaling pathway by small molecules, antibodies, or genetic manipulations may provide therapeutic strategy obesity. Here, we report active-like Cryo-EM structure human with...
At Singapore Polytechnic (SP), the Diploma in Perfumery and Cosmetic (DPCS) embraces an innovative Industry Now Curriculum (INC) pathway. With this framework, students engage industry projects to equip themselves with valuable knowledge skills crucial profession. This project-based learning (PBL) approach not only imparts practical expertise but also fosters autonomous through
Advances in singe-particle cryo-electron microscopy (cryo-EM) have made it possible to solve the structures of numerous Family A and B G protein-coupled receptors (GPCRs) complex with proteins arrestins, as well several C GPCRs. Determination these has been facilitated by presence large extramembrane components (such protein, arrestin, or Venus flytrap domains) complexes that aid particle alignment during processing cryo-EM data. In contrast, determination inactive state structure GPCRs is...
Aiming at the problem of high-precision segmentation skin lesions, a lesion network combining feature refinement and context guide is proposed. Firstly, dual-layer thinning module designed to mine difference information common between adjacent layers, generate weight vectors encoder map gradually refine, so as enhance ability expression. Secondly, dense residual pyramid highest level expand network's receptive field through cascading expansion convolution, integrate features different scales...