A5089673052- Receptor Mechanisms and Signaling
- Protein Structure and Dynamics
- interferon and immune responses
- Ubiquitin and proteasome pathways
- Advancements in Battery Materials
- Advanced battery technologies research
- Genetics and Neurodevelopmental Disorders
- Microbial Natural Products and Biosynthesis
- Protein Kinase Regulation and GTPase Signaling
- Advanced Battery Materials and Technologies
- Electron Spin Resonance Studies
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Research on Leishmaniasis Studies
- Advanced NMR Techniques and Applications
- Cancer-related Molecular Pathways
- SARS-CoV-2 and COVID-19 Research
- Inflammasome and immune disorders
- Enzyme Structure and Function
- Monoclonal and Polyclonal Antibodies Research
- Neuropeptides and Animal Physiology
- Chemical Synthesis and Analysis
- Mass Spectrometry Techniques and Applications
- Biochemical and Structural Characterization
- Biochemical and Molecular Research
Peking University
2016-2025
Beijing National Laboratory for Molecular Sciences
2017-2025
Beihang University
2018-2024
Beijing Advanced Sciences and Innovation Center
2018
Northeast Forestry University
2014
Florida State University
2010-2013
Hefei National Center for Physical Sciences at Nanoscale
2007
University of Science and Technology of China
2007
A simple strategy to synthesize ultrathin, amorphous and alloyed structural cobalt–vanadium hydr(oxy)oxide catalysts with enhanced water oxidation catalytic activity.
Abstract SARS-CoV-2 is the pathogen responsible for COVID-19 pandemic. The papain-like cysteine protease (PLpro) has been implicated in playing important roles virus maturation, dysregulation of host inflammation, and antiviral immune responses. multiple functions PLpro render it a promising drug target. Therefore, we screened library approved drugs also examined available inhibitors against PLpro. Inhibitor GRL0617 showed vitro IC 50 2.1 μM an effective inhibition cell-based assays....
Abstract Potassium-ion batteries (KIBs) are promising electrochemical energy storage systems because of their low cost and high density. However, practical exploitation KIBs is hampered by the lack high-performance cathode materials. Here we report a potassium manganese hexacyanoferrate (K 2 Mn[Fe(CN) 6 ]) material, with negligible content defects water, for efficient high-voltage K-ion storage. When tested in combination K metal anode, ]-based electrode enables cell specific 609.7 Wh kg −1...
Abstract Metal hexacyanoferrates (HCFs) are viewed as promising cathode materials for potassium‐ion batteries (PIBs) because of their high theoretical capacities and redox potentials. However, the development an HCF with cycling stability voltage retention is still impeded by unavoidable Fe(CN) 6 vacancies (V FeCN ) H 2 O in materials. Here, a repair method proposed that significantly reduces V content potassium manganese hexacyanoferrate (KMHCF) enabled reducibility sodium citrate removal...
Significance Recent structures of GPCRs in complex with G proteins provide important insights into protein activation by family A and B GPCRs; however, questions remain. We don’t fully understand the mechanism coupling specificity or promiscuity some GPCRs. The β 2 AR preferentially couples to s less efficiently i , yet AR-G has been shown play roles cardiac physiology. To better structural basis for preferential over we used NMR spectroscopy supporting MD simulations study conformational...
Abstract Advances in structural biology have provided important mechanistic insights into signaling by the transmembrane core of G-protein coupled receptors (GPCRs); however, much less is known about intrinsically disordered regions such as carboxyl terminus (CT), which highly flexible and not visible GPCR structures. The β 2 adrenergic receptor’s (β AR) 71 amino acid CT a substrate for kinases binds β-arrestins to regulate signaling. Here we show that AR directly inhibits basal...
Abstract The M2 muscarinic receptor (M2R) is a prototypical G-protein-coupled (GPCR) that serves as model system for understanding GPCR regulation by both orthosteric and allosteric ligands. Here, we investigate the mechanisms governing M2R signaling versatility using cryo-electron microscopy (cryo-EM) NMR spectroscopy, focusing on physiological agonist acetylcholine supra-physiological iperoxo, well positive modulator LY2119620. These studies reveal stabilizes more heterogeneous...
Abstract Over the past decade, concerns over sustainability of lithium‐ion batteries (LIBs) have arisen due to scarcity critical elements such as lithium (Li), nickel (Ni), and cobalt (Co), prompting exploration alternative complementary electrochemical energy storage technologies. Due more abundant resources compared contemporary LIBs potentially higher specific than emerging sodium‐ion (SIBs), potassium‐ion (PIBs) attracted intensive research interest a promising existing Nevertheless,...
Abstract Described is a facile helix‐nucleating template based on tethered aspartic acid at the N‐terminus [terminal (TD)]. The nucleating effect of subtly influenced by substituent end side‐chain‐end tether as indicated circular dichroism, nuclear magnetic resonance, and molecular dynamics simulations. Unlike most strategies, N‐terminal amine preserved, thus enabling further modification. Peptidomimetic estrogen receptor modulators (PERMs) constructed using this strategy show improved...
Intrinsically disordered proteins or intrinsically regions (IDPs) have gained much attention in recent years due to their vital roles biology and prevalence various human diseases. Although IDPs are perceived as attractive therapeutic targets, rational drug design targeting remains challenging because of conformational heterogeneity. Here, we propose a hierarchical computational strategy for IDP virtual screening (IDPDVS) applied it the discovery p53 transactivation domain I (TAD1) binding...
The capacity degradation mechanism of layered potassium vanadium oxide K<sub>0.5</sub>V<sub>2</sub>O<sub>5</sub> towards K-ion storage was unveiled and the cycling stability this material enhanced by reducing its long-range structural order.
Coronaviruses that can infect humans cause either common colds (HCoV-NL63, HCoV-229E, HCoV-HKU1, and HCoV-OC43) or severe respiratory symptoms (SARS-CoV-2, SARS-CoV, MERS-CoV). The papain-like proteases (PLPs) of SARS-CoV-2, MERS-CoV, HCoV-NL63 function in viral innate immune evasion have deubiquitinating (DUB) deISGylating activities. We identified the PLPs HCoV-OC43 found their enzymatic properties correlated with ability to suppress responses. A conserved noncatalytic aspartic acid...
RAS oncogenic mutations are pivotal drivers of tumorigenesis. Ubiquitination modulates functions, including activation, stability, and localization. While several E3 ligases regulate ubiquitination, deubiquitination remains less understood. Our study reveals that ubiquitin-specific protease 7 (USP7) directly deubiquitinates KRAS, stabilizing it promoting the proliferation non-small cell lung cancer (NSCLC) cells. Mechanistically, USP7 binds KRAS via its TRAF domain removes K48-linked...
β-Arrestins (βarrs) are functionally versatile proteins that play critical roles in the G-protein-coupled receptor (GPCR) signaling pathways. While it is well established phosphorylated tail plays a central role βarr activation, emerging evidence highlights contribution from membrane lipids. However, detailed molecular mechanisms of activation by different binding partners remain elusive. In this work, we present comprehensive study structural changes regions βarr1 during using 19F NMR...
Abstract The WRKY transcription factors play essential roles in a variety of plant signaling pathways associated with biotic and abiotic stress response. transcriptional activity many members are regulated by class intrinsically disordered VQ proteins. While it is known that proteins interact the DNA-binding domains (DBDs), also termed as domains, structural information regarding VQ-WRKY interaction lacking regulation mechanism remains unknown. Herein we report solution NMR study between...
The bacterial acid-resistant chaperone HdeA is a "conditionally disordered" protein that functions at low pH when it undergoes transition from well-folded dimer to an unfolded monomer. dissociation and unfolding processes result in exposure of hydrophobic surfaces allows binding broad range client proteins. To fully elucidate the mechanism HdeA, crucial understand how activated interacts with its native substrates during acid stress. Herein, we present nuclear magnetic resonance study...
Lasso peptides are unique natural products that comprise a class of ribosomally synthesized and post-translationally modified peptides. Their defining three-dimensional structure is lariat knot, in which the C-terminal tail threaded through macrolactam ring formed between N-terminal amino group an Asp or Glu side chain (i.e., isopeptide bond). Recent genome mining strategies have revealed various types lasso peptide biosynthetic gene clusters thus redefined known chemical space To date, over...
The human AF-6, a scaffold protein between cell membrane-associated proteins and the actin cytoskeleton, plays an important role in special cell-cell junctions signal transduction. It can be phosphorylated by kinase Bcr, which allows efficient binding of C terminus Bcr to PDZ domain AF-6 consequently enhances affinity Ras. Formation Ras ternary complex results down-regulation Ras-mediated transduction pathway. To better understand molecular basis for recognition we solve solution structure...
The periplasmic protein SurA is the primary chaperone involved in biogenesis of bacterial outer membrane proteins and a potential antibacterial drug target. three-dimensional structure can be divided into three parts, core module formed by N- C-terminal regions two peptidyl-prolyl isomerase (PPIase) domains, P1 P2. Despite determination structures several SurA-peptide complexes, functional mechanism this remains elusive roles PPIase domains are yet unclear. Herein, we characterize...