A5072695509- Histone Deacetylase Inhibitors Research
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Nuclear Receptors and Signaling
- Immune cells in cancer
- Advanced Biosensing Techniques and Applications
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Atherosclerosis and Cardiovascular Diseases
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- Phagocytosis and Immune Regulation
- Glycosylation and Glycoproteins Research
- Cancer-related gene regulation
The University of Texas MD Anderson Cancer Center
2024
High Magnetic Field Laboratory
2024
The University of Queensland
2021-2024
Hefei Institutes of Physical Science
2024
Chinese Academy of Sciences
2024
University of Science and Technology of China
2024
Air Force Medical University
2024
Abstract TLRs reprogram macrophage metabolism, enhancing glycolysis and promoting flux through the tricarboxylic acid cycle to enable histone acetylation inflammatory gene expression. The deacetylase (HDAC) family of lysine deacetylases regulates both TLR-inducible responses. Here, we show that TLR4 agonist LPS, as well agonists other TLRs, rapidly increase enzymatic activity class IIa HDAC (HDAC4, 5, 7, 9) in primary human murine macrophages. This response was abrogated macrophages...
Abstract In this study, we aimed to develop nanobodies targeting receptor tyrosine kinase‐like orphan 1 (ROR1) for cancer diagnosis and therapy. We immunized alpacas with ROR1, extracted RNA from their blood, converted it complementary DNA (cDNA) amplify the VHH (variable domain of heavy‐chain antibodies) sequence. This sequence was used construct a phage library capacity 8 ×10 . Screening identified high‐affinity nanobody, HCAbs1, which binds effectively ROR1. ELISA surface plasmon...
Abstract Hepatocellular carcinoma (HCC) is one of the most common malignant tumors digestive system; however, its etiology remains unclear. Clarifying pathogenesis essential to improve prognosis patients with HCC. Studies have shown that 3-methylcytidine (m3C) methylation regulator closely related occurrence and development has an excellent potential prognostic value. In present study, 486 HCC were collected from TCGA GEO databases, 16 adjacent tissues in our hospital collected. The...
Single-cell RNA sequencing (scRNA-seq) technology has been widely used to study the differences in gene expression at single cell level, providing insights into research of development, differentiation, and functional heterogeneity. Various pipelines workflows scRNA-seq analysis have developed but few considered multi-timepoint data specifically. In this study, we develop CASi, a comprehensive framework for analyzing multiple timepoints' data, which provides users with: (1) cross-timepoint...
Background: Chronic activation of the innate immune system drives inflammation and contributes to atherosclerosis. Pro-inflammatory agents (oxidised LDL, IL-1β) drive lesional development, whereas anti-inflammatory (IL-10, HDL, Apolipoprotein-AI), promote an milieu resolution. RIPK1 (Receptor interacting protein kinase 1) is a master regulatory gene that decides whether cell undergoes inflammation, apoptosis and/or necroptosis, depending on its phosphorylation or ubiquitination status. We...
Histone deacetylase 7 (HDAC7) is a member of the class IIa family classical HDACs with important roles in cell development, differentiation, and activation, including macrophages other innate immune cells. HDAC7 act as transcriptional repressors nucleus but, some types, they can also cytoplasm to modify non-nuclear proteins and/or scaffold signalling complexes. In macrophages, cytoplasmic protein both pro- anti-inflammatory functions, latter activity involving activation pentose phosphate...