A5038465975- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Diagnosis and treatment of tuberculosis
- Pneumonia and Respiratory Infections
- Pneumocystis jirovecii pneumonia detection and treatment
- Infectious Diseases and Tuberculosis
- Antibiotic Resistance in Bacteria
- Clinical Laboratory Practices and Quality Control
- Drug-Induced Hepatotoxicity and Protection
- Infectious Diseases and Mycology
- COVID-19 Clinical Research Studies
- Dialysis and Renal Disease Management
- Pharmacovigilance and Adverse Drug Reactions
- Biomedical Text Mining and Ontologies
- Parasites and Host Interactions
- Bacterial Identification and Susceptibility Testing
- Pharmacological Effects and Toxicity Studies
- Infections and bacterial resistance
- HIV/AIDS Research and Interventions
- Genomics and Phylogenetic Studies
- Malaria Research and Control
- COVID-19 diagnosis using AI
- Chronic Disease Management Strategies
- Leprosy Research and Treatment
- Enterobacteriaceae and Cronobacter Research
Ethiopian Public Health Institute
2016-2025
National Tuberculosis Institute
2023
Addis Ababa University
2020-2022
Hospital Universitario 12 De Octubre
2020
Emory University
2020
Multi drug resistant tuberculosis (MDR-TB) poses formidable challenges to TB control due its complex diagnostic and treatment often associated with a high rate of mortality. Accurate rapid detection MDR-TB is critical for timely initiation treatment. Line Probe Assay (LPA) qualitative in vitro test based on DNA-STRIP technology the identification M. resistance rifampicin (RMP) and/or isoniazid (INH). Hain Lifescience, GmbH, Germany has improved sensitivity Genotype MTBDRplus VER 2.0 LPA...
The diagnoses of active smear negative PTB, remains difficult. As a result, treatment is often carried out empirically relaying on clinical criteria. distribution and magnitude MDR-TB associated factors in the same day diagnosis strategy are not clearly known study area. Therefore, this aimed to determine prevalence TB, risk among presumptive pulmonary tuberculosis patients Addis Ababa, Ethiopia.Analytic cross sectional design was used. A total 418 TB were enrolled from selected health...
Young children cannot easily produce sputum for diagnosis of pulmonary tuberculosis (TB). Alternatively, Mycobacterium complex bacilli can be detected in stool by using the Xpert MTB/RIF (Ultra) assay (Xpert).
Bacteriological confirmed active case detection remains the corner stone for diagnosing tuberculosis. Non-radiometric liquid culture system Mycobacterium Growth Indicator Tube with automated interface had been recommended by expert groups in addition to conventional solid media such as Lowenstein-Jensen. However high burden resource limited countries advanced non-radiometric based tuberculosis diagnostic methods MGIT 960 is limited. Therefore we have evaluated performance of compared LJ...
Individuals with diabetes mellitus (DM) have high tuberculosis (TB) prevalence. Currently, the incidence of DM is increasing in low and middle-income countries including Ethiopia where TB also endemic, which may complicate prevention control efforts. In this context, prevalence associated factors among patients are not well understood Ethiopia. This study assessed attending public health facilities A multicenter cross-sectional was employed from January to December 2023 selected five regions...
Background There was a ‘missing millions’ gap between the incidence of tuberculosis (TB) cases and notified cases. In many TB high-burden countries, only about 25% household contacts (HHCs) completing evaluation 20–89% eligible did not adhere to screening. The study conducted assess yield door-to-door contact investigation among bacteriologically confirmed pulmonary in central Ethiopia. Methods This cross-sectional carried out selected health facilities Ethiopia from January 1, 2023 December...
Rapid and sensitive Tuberculosis (TB) diagnosis closer to patients is a key global TB control priority. Truenat assays (MTB, MTB Plus, MTB-RIF Dx) are new molecular diagnostic tools for the detection of Rifampicin (RIF)-resistance from sputum samples. The accuracy needed prior implementation in clinical use Ethiopia. This study aimed determine sensitivity specificity assays; compare Xpert MTB/RIF assay.
Background Drug resistance is becoming a major bottleneck for tuberculosis (TB) control programs in countries with high TB burdens. Although several studies were conducted on the drug sensitivity of Mycobacterium ( M. ) central Ethiopia, there lack data peripheral regions country including Somali region. Therefore, objective this study was to evaluate and its association bacterial genotype performance Xpert MTB/RIF (Xpert) detecting rifampicin (RIF). Methods A total 302 tested using BD...
Drug-resistance in Mycobacterium tuberculosis complex remains a major health burden human history and still is leading cause of death developing countries including Ethiopia. Early detection all forms drug-resistant Tuberculosis(TB) key factor to reduce contain the spread these resistant strains.
Abstract The Mycobacterium tuberculosis complex (MTBC) includes several human- and animal-adapted pathogens. It is thought to have originated in East Africa from a recombinogenic canettii -like ancestral pool. Here, we describe the discovery of clinical strain isolated Ethiopia that shares archetypal phenotypic genomic features M. strains, but represents phylogenetic branch much closer MTBC clade than strains. Analysis traces horizontal gene transfer this isolate previously identified...
Introduction In Ethiopia, >300 GeneXpert instruments have been deployed for tuberculosis (TB) testing using the Xpert MTB/RIF cartridge. Implementing quality indicators is necessary monitoring and evaluating of diagnostic services. Objective To assess use molecular assay in Ethiopia to compare findings with predefined targets described literature. Methods Clinical specimens collected from patients suspected TB were subjected at National Reference Laboratory (NTRL) between January December...
In co-endemic areas, rate of intestinal parasites and tuberculosis (TB) co-infection thought to be high. However, there are limited studies on the epidemiology this in Ethiopia. Therefore, present study aimed generate evidence associated factors among pulmonary patients (PTB) their household contacts Addis Ababa, Unmatched case-control was conducted. Data were collected from 91 PTB (cases) 89 (controls). Socio-demographic characteristics using structured questionnaire. Sputum, stool blood...
Both passive and active surveillance of drug resistance have an important role in tuberculosis (TB) control program. Surveillance data are to estimate the magnitude TB, know trend disease, assess performance program, forecast diagnosis treatment supplies. Therefore, this study aimed determine prevalence proportion resistant Ethiopia based on passively collected data. A cross-sectional was conducted at National Tuberculosis Reference Laboratory seven Regional TB laboratories a retrospective...
Background Childhood tuberculosis (TB) diagnosis remains challenging, partly because children cannot provide sputum. This study evaluated the diagnostic accuracy of Simple One-Step (SOS) stool method with Xpert MTB/RIF Ultra (Xpert-Ultra) for childhood TB compared to culture and Xpert-Ultra on a respiratory sample (RS) clinical diagnosis. It also assessed feasibility acceptability testing according laboratory staff, caregivers’ preference. Methods We enrolled (≤10 years) presumptive...
Ethiopia is one of the countries with a high tuberculosis (TB) burden, yet little known about spatial distribution Mycobacterium (Mtb) lineages. This study identifies spoligotyping 1735 archived Mtb isolates from National Drug Resistance Survey, collected between November 2011 and June 2013, to investigate population structure distribution. Spoligotype International Types (SITs) lineages were retrieved online databases. The was evaluated using Fisher's exact test logistic regression models....
The rise of drug-resistant tuberculosis (DR-TB) has presented a substantial challenge to the national (TB) control program. Understanding epidemiology pre-extensively (pre-XDR-TB) could help clinicians adapt MDR-TB treatment regimens at an earlier stage. This study aimed assess second-line anti-TB drug resistance among patients in Ethiopia using routine laboratory-based data.
Background Isoniazid (INH) resistant Mycobacterium tuberculosis (Hr-TB) is the most common type of drug TB, and defined as M complex (MTBC) strains to INH but susceptible rifampicin (RIF). Resistance precedes RIF resistance in almost all multidrug TB (MDR-TB) cases, across MTBC lineages settings. Therefore, early detection Hr-TB critical ensure rapid initiation appropriate treatment, prevent progression MDR-TB. We assessed performance GenoType MTBDR plus VER 2.0 line probe assay (LPA)...