A5038634948- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Diagnosis and treatment of tuberculosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Pneumonia and Respiratory Infections
- HIV/AIDS Research and Interventions
- Viral Infections and Outbreaks Research
- Clinical Laboratory Practices and Quality Control
- Antibiotic Resistance in Bacteria
- Infectious Diseases and Mycology
- Immune responses and vaccinations
- Bacterial Identification and Susceptibility Testing
- HIV/AIDS Impact and Responses
- Infectious Diseases and Tuberculosis
- HIV Research and Treatment
- Genomics and Phylogenetic Studies
Ethiopian Public Health Institute
2019-2025
Jimma University
2024
National Tuberculosis Institute
2023
Addis Ababa University
2022
Rapid and sensitive Tuberculosis (TB) diagnosis closer to patients is a key global TB control priority. Truenat assays (MTB, MTB Plus, MTB-RIF Dx) are new molecular diagnostic tools for the detection of Rifampicin (RIF)-resistance from sputum samples. The accuracy needed prior implementation in clinical use Ethiopia. This study aimed determine sensitivity specificity assays; compare Xpert MTB/RIF assay.
Background Drug resistance is becoming a major bottleneck for tuberculosis (TB) control programs in countries with high TB burdens. Although several studies were conducted on the drug sensitivity of Mycobacterium ( M. ) central Ethiopia, there lack data peripheral regions country including Somali region. Therefore, objective this study was to evaluate and its association bacterial genotype performance Xpert MTB/RIF (Xpert) detecting rifampicin (RIF). Methods A total 302 tested using BD...
Extrapulmonary tuberculosis is an emerging public health problem among HIV positives compared to the general population. This study aimed assess incidence and predictors of extrapulmonary people living with in selected facilities Addis Ababa, Ethiopia, from 01 January 2013 up 31 December 2018.A retrospective cohort design was employed based on data collected 566 positive individuals. Data were entered using EpiInfo version 7.1 analyzed by SPSS 20. The rate determined per 100 person-years....
To assess the incidence and determinants of tuberculosis (TB) among HIV-positive individuals in selected health facilities Addis Ababa, Ethiopia, during period January 2013 to December 2018.Data were collected from records 566 individuals. A retrospective cohort study design was employed. Data entered into Epi Info 7 analyzed using IBM SPSS Statistics version 20. TB density determined per 100 person-years. Time-to-event distributions estimated Kaplan-Meier estimates. Survival curves hazards...
Drug-resistance in Mycobacterium tuberculosis complex remains a major health burden human history and still is leading cause of death developing countries including Ethiopia. Early detection all forms drug-resistant Tuberculosis(TB) key factor to reduce contain the spread these resistant strains.
Abstract The Mycobacterium tuberculosis complex (MTBC) includes several human- and animal-adapted pathogens. It is thought to have originated in East Africa from a recombinogenic canettii -like ancestral pool. Here, we describe the discovery of clinical strain isolated Ethiopia that shares archetypal phenotypic genomic features M. strains, but represents phylogenetic branch much closer MTBC clade than strains. Analysis traces horizontal gene transfer this isolate previously identified...
The rise of drug-resistant tuberculosis (DR-TB) has presented a substantial challenge to the national (TB) control program. Understanding epidemiology pre-extensively (pre-XDR-TB) could help clinicians adapt MDR-TB treatment regimens at an earlier stage. This study aimed assess second-line anti-TB drug resistance among patients in Ethiopia using routine laboratory-based data.
This study aimed to determine the frequencies and trends of Mycobacterium tuberculosis rifampicin resistance among presumptive patients in Ethiopia, who were tested using Xpert MTB/RIF assay between 2014 2021. Data collected retrospectively from patient registries. Laboratory-based data extracted national (TB) referral laboratory database. All referred National Tuberculosis Reference Laboratory (NTRL) for TB diagnosis all over country March 1, September 30, 2021, assay, included. The entered...
Laboratory identification of nontuberculous mycobacteria (NTM) species is not regularly performed while, they have a public health importance with prevalence more than 5% among pulmonary tuberculosis (PTB) patients in Ethiopia. Hence, this study aimed to identify the NTM and their clinical significance PTB patients. A retrospective was conducted at Ethiopian Public Health Institution's (EPHI's) national TB referral laboratory. Stored isolates were genotyped using GenoType Mycobacterium CM/AS...
The introduction of antiretroviral therapy (ART) significantly decreases the incidence tuberculosis (TB) in people living with human immunodeficiency virus (PLWHIV). However, a considerable proportion is still co-infected TB after ART initiation. Thus, this study aimed to assess effect long-term HAART on among PLWHIV Addis Ababa, Ethiopia.A matched nested case-control was conducted who were enrolled clinics Ethiopia from 2013 up 2018. Cases HIV-TB individuals taking treatment, while controls...
Introduction The quality of tuberculosis laboratory services in health facilities is a mandatory component detecting active pulmonary TB cases and treatment follow-up. However, ensuring the test results concern. This study aimed to assess assurance practices diagnostic Ethiopia. Materials methods A cross-sectional was conducted from October 2018 March 2019 at nine governmental TB-culture laboratories 34 randomly selected GeneXpert® MTB/RIF (Xpert® MTB/RIF) testing Participating were...
Background: The introduction of antiretroviral therapy (ART) significantly decreases the incidence tuberculosis (TB) in Human Immunodeficiency Virus ( HIV ) positive individuals during their follow-up. However, a considerable proportion is still co-infected with TB after ART initiation. Thus, this study aimed to assess determinants among -positive follow-up Addis Ababa, Ethiopia. Methods: A nested case-control was conducted HIV- who were enrolled clinics Ethiopia from 2013 up 2018. Cases...
<title>Abstract</title> Background Smear positive TB cases greatly contribute to community level transmission of the disease. Locating hotspots would make it easier prioritize and target control interventions. This study is aimed at assessing spatial distribution smear index their secondary predictors clustering cases. Methods The was conducted in Silti Zone Central Ethiopia from 2020–2022. Data were collected unit registers healthcare facilities. Contacts all screened tested identify We...
Smear-positive TB patients greatly contribute to community-level transmission of this disease. Locating hotspots would make it easier prioritize and target control interventions. This study aimed assess the spatial distribution smear-positive index cases their secondary predictors clustering cases. was conducted in Silti Zone Central Ethiopia from 2020 2022. The data were collected unit registries healthcare facilities. Contacts all screened community tested identify patients. We performed...
Abstract Background Conventional wisdom wrongly holds that the microbiological of M. tuberculosis complex in clinical specimens via culture and phenotypic drug susceptibility testing allows people to be correctly diagnosed ensures an effective treatment regimen selected. This study was aimed characterize first-and second-line anti-tuberculosis resistance profiles among new pulmonary cases Addis Ababa metropolitan area, Ethiopia. Methods A prospective cross-sectional conducted between October...
Abstract Background: Xpert MTB/RIF Assay is currently used in Ethiopia for the rapid diagnosis of Mycobacterium tuberculosis (MTB) and mutations that confer Rifampicin resistance. resistance determined based on any mutation 81 bp rpoB gene using five overlapping probes represented as Probe A (codons 507–511), B 512–518), C 518–523), D 523–529) E 529–533). In this review, we assessed frequency missed probe types Resistance results. Methods: Data were reviewed from specimens received tested...
Although homeless segment of the society could be hotspots for tuberculosis (TB) transmission, there is little data on TB in individuals Ethiopia. The objective this study was to investigate molecular epidemiology and drug sensitivity Mycobacterium (M. tuberculosis) isolated from Addis Ababa, conducted 59 M. isolates, which were recovered by clinical screening 5600 bacteriological examination 641 with symptoms pulmonary (PTB). Region difference-9 (RD9) based polymerase-chain reaction (PCR),...
Abstract Background: Xpert® MTB/RIF assay is currently used in Ethiopia for the rapid diagnosis of Mycobacterium tuberculosis (MTB) and mutations that confer Rifampicin resistance. resistance determined based on any mutation 81 bp rpoB gene using five overlapping probes represented as Probe A (codons 507–511), B 512–518), C 518–523), D 523–529) E 529–533). In this review, we assessed frequency missed probe types Resistance results. Methods: Data were reviewed from specimens received tested...
Background Mycobacterium tuberculosis ( M . ) remains one of the most significant causes death and a major public health problem in community. As result, aim this study was to determine magnitude , its drug resistance, associated factors among presumptive (TB) patients at St. Paul’s Hospital Millennium Medical College, Addis Ababa, Ethiopia. Methods Cross-sectional conducted College (SPHMMC), Ethiopia from January July 2019. Demographic clinical data were collected by structured...