A5016005918- Animal Ecology and Behavior Studies
- Animal Behavior and Welfare Studies
- Animal testing and alternatives
- Bat Biology and Ecology Studies
- Adipose Tissue and Metabolism
- Neuroendocrine regulation and behavior
- Birth, Development, and Health
- Medical Imaging and Pathology Studies
- Medical Imaging Techniques and Applications
- Animal Vocal Communication and Behavior
- Veterinary Pharmacology and Anesthesia
- Prostate Cancer Treatment and Research
- Neonatal Respiratory Health Research
- Rabbits: Nutrition, Reproduction, Health
- Food Safety and Hygiene
- Biomedical Ethics and Regulation
- Olfactory and Sensory Function Studies
- Inflammatory Bowel Disease
- Animal Nutrition and Physiology
- Physiological and biochemical adaptations
- Identification and Quantification in Food
- Ethics in Clinical Research
- Animal Disease Management and Epidemiology
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2019-2024
Universidade do Porto
2020-2024
The Francis Crick Institute
2021-2022
Instituto de Biologia Molecular e Celular
2018-2021
Institute of Biomedical Science
2018
The ongoing worldwide effort to reduce animal numbers in research often omits the issue of pre-weaning mortality mouse breeding. A conservative estimate 20% would mean approximately 1.1 M mice die annually EU before scientific use. We hypothesize that laboratory breeding is associated with cage social and macro/micro-environment conditions. Here we count pups from 509 C57BL/6J litters daily for accurate detection mortality, monitor micro-environment 172 litters. Probability increases...
Perinatal mortality is a major issue in laboratory mouse breeding. We compared counting method using daily checks (DAILY_CHECK) with combining detailed video analyses to detect cannibalisms (VIDEO_TRACK) for estimating the number of C57BL/6 pups that were born, died and weaned 193 litters from trios (TRIO-OVERLAP) or without (TRIO-NO_OVERLAP) presence another litter. Linear mixed models used at litter level. To understand whether cannibalism was associated active killing (infanticide), we...
High and variable pre-weaning mortality is a persistent problem in laboratory mouse breeding. Assuming modest 15% rate across strains, means that approximately 1 million more pups are produced yearly the EU to compensate for those which die. This paper presents first large study under practical husbandry conditions determine risk factors associated with mortality. We analysed historical records from 219,975 two breeding facilities, collected as part of their management routine including...
Abstract Neonatal mortality in wild-type laboratory mice is an overlooked welfare and financial problem animal facilities around the world. Causes of death are often not reported its causes remain unknown. In this study, 324 newborn pups from two breeding colonies healthy wildtype C57BL/6 underwent post-mortem analysis with special focus on obtaining proof life after birth, evaluation stomach contents observation congenital abnormalities that could compromise survival. Based a combination...
Can nonhuman animals be used for the benefit of humans in a scientifically and morally justified manner and, if yes, how? Based on our own experiences as scholars from various academic backgrounds, we argue that this question can only answered an interdisciplinary international endeavor, considering insights research ethics animal well scientific legal aspects. The aim article is to contribute foundation emerging field ethics. In doing so, describe following seven phases experiments:...
Neonatal mortality is high in laboratory mouse breeding, and causes are poorly understood. Post-mortem analysis of pups an often overlooked source information insight. We present a necropsy protocol for neonatal mice designed easy practical application by animal technicians.
Reproducibility in animal research is crucial for its reliance and translational relevance. The 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced model of inflammatory bowel disease (IBD) widely used but inconsistently incompletely characterized throughout the literature. This hinders comparisons between studies influences low rate translation effective preclinical molecules. purpose this study was to categorize TNBS-induced colitis, based on macroscopic microscopic scoring systems,...
Perinatal mortality is a major issue in laboratory mouse breeding. We compared counting method using daily checks (DAILY_CHECK) with combining detailed video analyses to detect cannibalisms (VIDEO_TRACK) for estimating the number of C57BL/6 pups born, died and weaned 193 litters from trios (TRIO-OVERLAP) or without (TRIO-NO_OVERLAP) presence another litter. Linear mixed models were used at litter level. To understand if cannibalism was associated active killing (infanticide), we analysed...
Abstract High and variable pre-weaning mortality is a persistent problem among the main mouse strains used in biomedical research. If modest 15% rate assumed across all EU, approximately 1 million more pups must be produced yearly to compensate for those which die. A few environmental social factors have been identified as affecting pup mortality, but optimizing these does not cease problem. This study first large mine data records from 219,975 two breeding facilities determine major risk...