A5048322592- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- RNA and protein synthesis mechanisms
- Innovation and Socioeconomic Development
- Microbial Community Ecology and Physiology
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Plant Molecular Biology Research
- GABA and Rice Research
- RNA regulation and disease
- Advanced Computational Techniques and Applications
- Biochemical and Structural Characterization
- Insect symbiosis and bacterial influences
- Security and Verification in Computing
- Environmental DNA in Biodiversity Studies
- Cancer Immunotherapy and Biomarkers
- Plant Pathogenic Bacteria Studies
- Animal Genetics and Reproduction
- Global Trade and Competitiveness
- Genetic Mapping and Diversity in Plants and Animals
- Plant-Microbe Interactions and Immunity
- Cancer-related molecular mechanisms research
Shanghai Jiao Tong University
2022-2025
Guangxi Academy of Agricultural Science
2005-2024
Chinese Academy of Sciences
2011-2023
Center for Excellence in Molecular Plant Sciences
2016-2023
City University of Hong Kong
2023
Wuhan University
2013
Institute of Hydrobiology
2011-2013
Kunming Institute of Zoology
2013
University of Chinese Academy of Sciences
2013
Seed germination and flowering initiation are both transitions responding to similar seasonal cues. This study shows that ABSCISIC ACID-INSENSITIVE MUTANT 5 (ABI5), a bZIP transcription factor, which plays an important role in the abscisic acid (ABA)-arrested seed germination, is robustly associated with floral transition Arabidopsis. Under long-day conditions, overexpression of ABI5 could delay through upregulating FLOWERING LOCUS C (FLC) expression. In contrast, ectopically overexpressing...
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The CRISPR/Cas-mediated genome editing technology has been widely applied to create knockout alleles of genes by generating short insertions or deletions (indel) in various plant species. Due the low efficiency homology-directed repair (HDR) and difficulties delivery DNA template for HDR, precise remains challenging plants (Mao et al., 2019). A tandem repeat-HDR method was developed very recently sequence replacement rice, which is most useful monocots (Lu 2020). Base editors from Cas9...
Cytosine and adenosine base editors (CBE ABE) have been vigorously developed in plants, but the conversion types are limited (Ren et al., 2018, 2021; Xu 2021). A new editor CGBE with a uracil DNA N-glycosylase (UNG) has recently reported that enables efficient C-to-G editing mammalian cells (Kurt In generating more substitution can expand its application, help create germplasm resources (Bharat 2020; Butt Molla Yang, 2019). Currently, CGBEs applicable to plants yet be developed. Here, we...
The spatiotemporal knockdown of genes through genome editing heralds a new frontier in molecular breeding, yet it remains largely unexplored. Recognizing the intricate regulatory networks endogenous microRNAs (miRNAs), we posited that integrating specific miRNA target sequences into 3' UTR gene could construct artificial miRNA-dependent circuits, facilitating precise suppression. To test this hypothesis, selected three miRNAs with unique expression profiles by analyzing rice profiles....
The ciliated protozoan Tetrahymena thermophila is a useful unicellular model organism for studies of eukaryotic cellular and molecular biology. Researches on T. have contributed to series remarkable basic biological principles. After the macronuclear genome was sequenced, substantial progress has been made in functional genomics research thermophila, including genome-wide microarray analysis life cycle, gene network based data transcriptome by deep RNA sequencing. To meet growing demands...
A circular plasmid containing a gene coding sequence has been broadly used for studying regulation in cells. However, to accommodate quick screen construction and preparation can be time consuming. Here we report PCR amplified dsDNA fragments (PCR-fragments) based transient expression system (PCR-TES) suiting the study of plant Instead transforming plasmids into cells, PCR-fragments applicable. The transformation efficiency property are comparable using plasmids. We analyzed PCR-TES at...
Tetrahymena thermophila is a model eukaryotic organism. Functional genomic analyses in present rich opportunities to address fundamental questions of cell and molecular biology. The Gene Expression Database (TGED; available at http://tged.ihb.ac.cn) the first expression database ciliated protozoan. It covers three major physiological developmental states: growth, starvation, conjugation, can be accessed through user-friendly web interface. gene profiles candidate co-expressed genes for each...
Gene silencing is crucial in crop breeding for desired trait development. RNA interference (RNAi) has been used widely but limited by ectopic expression of transgenes and genetic instability. Introducing an upstream start codon (uATG) into the 5'untranslated region (5'UTR) a target gene may 'silence' inhibiting protein translation from primary (pATG). Here, we report efficient method introducing tailor-designed uATG-containing element (ATGE) 5'UTR genes plants, occupying original site to act...
With the widespread use of clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated nuclease (Cas) technologies in plants, large-scale genome editing is increasingly needed. Here, we developed a geminivirus-mediated surrogate system, called Wheat Dwarf Virus-Gate (WDV-surrogate), to facilitate high-throughput editing. WDV-Gate has two parts: one recipient callus from transgenic rice line expressing Cas9 and mutated hygromycin-resistant gene (HygM) for selection; other...
In-locus editing of agronomically-important genes to optimize their spatiotemporal expression is becoming an important breeding approach. Compared intensive studies on mRNA transcription, manipulating protein translation by genome has not been well exploited. Here, we found that precise knock-in a regulating element into the 5'UTR target gene could efficiently increase its abundance in rice. We firstly screened translational enhancer (AMVE) from alfalfa mosaic virus using protoplast-based...
Base editors, including cytosine and adenine base editors (CBE ABE), are promising tools for precise genome modification. They enable the generation of single nucleotide variants in plants research crop improvement (Li et al., 2020; Manghwar 2019; Ren 2021; Xu Zeng 2022). However, existing still limited types conversions they can induce. Recently, a new editor was constructed by fusing an engineered N-methylpurine DNA glycosylase (MPG) with ABE to create AYBE. This has achieved efficient...
Pollen serves as an essential protein source for honey bee larvae. The nutrients in pollen greatly influence larval growth and development. Here, the survival, prepupal weight, developmental stage, digestibility midgut cells ( Apis mellifera L.) larvae were evaluated by performing vitro 5-ethynyl-2′-deoxyuridine (EdU) assays on reared three single pollens Brassica napus L., Armeniaca sibirica Pyrus bretschneideri Rehd.) a mixture (mixture of equal proportions). results showed that survival...