A5072013749- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Plant Reproductive Biology
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- GABA and Rice Research
- Genetic Mapping and Diversity in Plants and Animals
- Research in Cotton Cultivation
- Biopolymer Synthesis and Applications
- Cancer Research and Treatments
- Photosynthetic Processes and Mechanisms
- Wheat and Barley Genetics and Pathology
- Colorectal Cancer Treatments and Studies
- Evacuation and Crowd Dynamics
- Plant Genetic and Mutation Studies
- Dye analysis and toxicity
- Microbial bioremediation and biosurfactants
- Genetics, Aging, and Longevity in Model Organisms
- Biosimilars and Bioanalytical Methods
- Phytase and its Applications
- Advanced biosensing and bioanalysis techniques
- Agronomic Practices and Intercropping Systems
- Plant Surface Properties and Treatments
Huazhong Agricultural University
2019-2024
Beike Biotechnology (China)
2024
Zhejiang University
2023
Chinese PLA General Hospital
2020-2022
Shanghai Jiao Tong University
2009-2010
Maize (
Abstract Fruits and vegetables in the Cucurbitaceae family, such as cucumber, melon, watermelon, squash, contribute greatly to human diet. The widespread use of genome editing technologies has accelerated gene functional characterization crop improvement. However, most economically important cucurbit plants, including melon remain recalcitrant standard Agrobacterium tumefaciens-mediated transformation, limiting effective technology. In this study, we used an “optimal infiltration intensity”...
Cotton (Gossypium spp.) is one of the most important fiber crops worldwide. In last two decades, transgenesis and genome editing have played roles in cotton improvement. However, genotype dependence key bottlenecks generating transgenic gene-edited plants through either particle bombardment or Agrobacterium-mediated transformation. Here, we developed a shoot apical meristem (SAM) cell-mediated transformation system (SAMT) that allowed recalcitrant genotypes including widely grown upland...
Kernel dehydration rate (KDR) is a crucial production trait that affects mechanized harvesting and kernel quality in maize; however, the underlying mechanisms remain unclear. Here, we identified quantitative locus (QTL), qKDR1, as non-coding sequence regulates expression of qKDR1 REGULATED PEPTIDE GENE (RPG). RPG encodes 31 amino acid micropeptide, microRPG1, which controls KDR by precisely modulating two genes, ZmETHYLENE-INSENSITIVE3-like 1 3, ethylene signaling pathway kernels after...
Summary Low grain moisture at harvest is crucial for safe production, transport and storage, but the genetic architecture of this trait in maize ( Zea mays ) remains elusive. Here, we measured dynamic changes content an association‐mapping panel 513 diverse inbred lines five successive stages across geographical environments. Genome‐wide association study (GWAS) revealed 71 quantitative loci (QTLs) that influence maize. Epistatic effects play vital roles variability levels, even...
Phytophthora sojae causes root and stem rot disease of soybean (Glycine max), leading to huge annual yield loss worldwide, but resistance (Rps) genes remains elusive. Soybean cultivar "Yudou 29" is resistant P. strain PsMC1, this study aimed clone, identify, characterize the Rps gene in Yudou 29 (RpsYD29) clarify its functional mechanism. We map-based cloned RpsYD29 (ZINC FINGER PROTEIN03, GmZFP03) using families a cross between sojae-susceptible "Jikedou 2". GmZFP03 was functionally...
Identifying transcriptional cis-regulatory elements (CREs) and understanding their role in gene expression are essential for the precise manipulation of associated phenotypes. This knowledge is fundamental advancing genetic engineering improving crop traits. We here demonstrate that CREs can be accurately predicted utilized to precisely regulate beyond range natural variation. firstly build two sequence-to-expression deep learning models respectively identify distal proximal by combining...
In maize (Zea mays), kernel weight is an important component of yield that has been selected during domestication. Many genes associated with have identified through mutant analysis. Most are involved in the biogenesis and functional maintenance organelles or other fundamental cellular activities. However, few quantitative trait loci (QTLs) underlying variation cloned. Here, we characterize a QTL, qKW9, weight. This QTL encodes DYW motif pentatricopeptide repeat protein C-to-U editing ndhB,...
The nutritional traits of maize kernels are important for human and animal nutrition, these have undergone selection to meet the diverse needs humans. However, our knowledge genetic basis selecting kernel is limited. Here, we identified both single epistatic quantitative trait loci (QTLs) that contributed differences oil carotenoid between teosinte. Over half teosinte alleles QTLs increased values detected traits. Based on pleiotropism or linkage information QTLs, constructed a trait-locus...
Abstract Unilateral cross incompatibility (UCI) occurs between popcorn and dent corn, represents a critical step towards speciation. It has been reported that ZmGa1P , encoding pectin methylesterase ( PME ), is male determinant of the Ga1 locus. However, female genetic relationship determinants at this locus are unclear. Here, we report three different types, total seven linked genes underlying locus, which control UCI phenotype by independently affecting pollen tube growth in both...
Base editors, including cytosine and adenine base editors (CBE ABE), are promising tools for precise genome modification. They enable the generation of single nucleotide variants in plants research crop improvement (Li et al., 2020; Manghwar 2019; Ren 2021; Xu Zeng 2022). However, existing still limited types conversions they can induce. Recently, a new editor was constructed by fusing an engineered N-methylpurine DNA glycosylase (MPG) with ABE to create AYBE. This has achieved efficient...
The emerging and promising biotechnology methodology of CRISPR/Cas9 gene editing is revolutionizing crop improvement. However, the low-efficiency, time-consuming labour-intensive nature positive or/and negative selections during or after transformation subsequent identification mutations are major challenges for its agricultural application, from upstream (high-throughput) mutant screening to downstream commercial production (He Zhao, 2020). Although visual markers, in particular fluorescent...
Cotton fibres originate from the outer ovule integument and D-lineage genes are essential for development their roles can be described by 'ABCDE' model of flower development. To investigate role during fibre development, GbAGL1 (GenBank accession number: FJ198049) was isolated G. barbadense using SMART RACE strategy. Sequence phylogenetic analyses revealed that a member gene family. Southern blot analysis showed belonged to low-copy Semi-quantitative RT-PCR RNA in situ hybridization highly...
A phase II/III study, is being conducted to evaluate the efficacy and safety of sintilimab, a PD-1 blockade, plus IBI305, bevacizumab biosimilar, comparing with sorafenib as first-line treatment for advanced HCC (NCT03794440). In II, run-in part, adults or metastatic (histologically diagnosed clinical cirrhosis per AASLD guideline) were enrolled receive sintilimab (200 mg IV Q3W) IBI305 (15 mg/kg Q3W). The primary endpoint profile. III following same inclusion exclusion criteria, it planned...
Since 2022, several large-scale epidemic outbreaks have occurred in various parts of the world, posing a risk interruption emergency supplies and potential disruptions supply chain. Therefore, selecting appropriate points is crucial to mitigate these risks. The selection distribution must consider multiple objectives, such as labor economic costs, personnel aggregation, mobility. To address challenges, this paper proposed hybrid approach that combines cellular automata K-means algorithms...
Abstract Agrobacterium–mediated transformation is a cost-effective and convenient way to introduce foreign genetic elements plants. However, only limited protocols successfully generates transgenic plants with fielder, spring wheat variety. Wheat method higher efficiency without genotype restriction heavily demanded. Here, heat-shock protocol, independent of Japan Tobacco had been established. Transgenic can be obtained from immature embryo within 60 days by this protocol. Morphogenic...
Abstract In this study, a novel radio‐frequency cold plasma‐assisted extraction (CPAE) process was developed for the of anthocyanins from type agricultural biomass waste, purple corncob. The effects plasma pretreatment on anthocyanin were investigated, and further optimized using response surface methodology. components corncob (PCAs) analyzed by super‐high performance liquid chromatography‐mass spectrum. antioxidant activity PCAs evaluated through 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH)...
Double haploid (DH) technology can be used to rapidly develop homozygous lines (Geiger and Gordillo, 2009). As the fundamental component of DH technology, traditional inducer were developed through a process recurrent selection over multiple generations, method that was inherently time-consuming. The advent gene editing has facilitated creation in an efficient manner (Kelliher et al., 2017; Zhong 2019). However, these lack sort markers for sorting haploid, introduction genetic is achieved...