A5101483296- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Genetic and phenotypic traits in livestock
- Wheat and Barley Genetics and Pathology
- Genomics and Phylogenetic Studies
- Animal Virus Infections Studies
- Plant Disease Resistance and Genetics
- Virus-based gene therapy research
- Environmental and Analytical Chemistry Studies
- Remote Sensing and Land Use
- Plant nutrient uptake and metabolism
- Forest, Soil, and Plant Ecology in China
- Viral gastroenteritis research and epidemiology
- GABA and Rice Research
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- SARS-CoV-2 and COVID-19 Research
- Rice Cultivation and Yield Improvement
- Plant and animal studies
- Crop Yield and Soil Fertility
- Plant Reproductive Biology
- melanin and skin pigmentation
- Plant Molecular Biology Research
Guizhou Normal University
2025
Guangxi Academy of Agricultural Science
2019-2024
Rice Research Institute
2009-2024
Institute of Crop Sciences
2020
Chinese Academy of Agricultural Sciences
2020
Huazhong Agricultural University
2012-2020
Chongqing Academy of Agricultural Sciences
2020
Qingdao Agricultural University
2012-2020
Southeast University
2017
Entry Exit Inspection and Quarantine Bureau
2013
Association mapping is a powerful approach for dissecting the genetic architecture of complex quantitative traits using high-density SNP markers in maize. Here, we expanded our association panel size from 368 to 513 inbred lines with 0.5 million high quality SNPs two-step data-imputation method which combines identity by descent (IBD) based projection and k-nearest neighbor (KNN) algorithm. Genome-wide studies (GWAS) were carried out 17 agronomic applying both mixed linear model (MLM) new...
Maize is one of the most important crops globally, and it shows remarkable genetic diversity. Knowledge this diversity could help in crop improvement; however, gold-standard genomes have been elucidated only for modern temperate varieties. Here, we present a high-quality reference genome (contig N50 15.78 megabases) maize small-kernel inbred line, which derived from tropical landrace. Using haplotype maps B73, Mo17 SK, identified 80,614 polymorphic structural variants across 521 diverse...
Kernel row number (KRN) is an important component of yield during the domestication and improvement maize controlled by quantitative trait loci (QTL). Here, we fine-mapped a major KRN QTL, KRN4, which can enhance grain productivity increasing per ear. We found that ~3-Kb intergenic region about 60 Kb downstream from SBP-box gene Unbranched3 (UB3) was responsible for variation in regulating level UB3 expression. Within 3-Kb region, 1.2-Kb Presence-Absence variant to be strongly associated...
Maize (Zea mays) is a major staple crop. kernel size and weight are important contributors to its yield. Here, we measured length, width, thickness, hundred weight, test in 10 recombinant inbred line populations dissected their genetic architecture using three statistical models. In total, 729 quantitative trait loci (QTLs) were identified, many of which identified all models, including 22 QTLs that each can explain more than 10% phenotypic variation. To provide candidate genes for these...
Abstract Maize seedlings are very sensitive to chilling, especially during the transition phase from heterotrophic autotrophic growth. Genetic dissection of genetic basis chilling tolerance would provide useful information for improvement maize inbreds. In this study, genome‐wide association analysis was conducted explore architecture at seed germination and seedling stages with an panel 125 Ten indices (ratios performance 10 rates growth‐related traits under stress control conditions) were...
Tartary buckwheat is an important medicinal and edible crop known for its significant health benefits to humans. While numerous germplasm resources have been collected in China, the genetic diversity core remain largely unclear. The aim of this work was analyze variability construct a collection buckwheat. Fifteen highly polymorphic SSR markers were used investigate 659 accessions. A total 142 alleles marked, with average 9.47 per locus. Genetic analysis revealed that these accessions...
In maize (Zea mays), kernel weight is an important component of yield that has been selected during domestication. Many genes associated with have identified through mutant analysis. Most are involved in the biogenesis and functional maintenance organelles or other fundamental cellular activities. However, few quantitative trait loci (QTLs) underlying variation cloned. Here, we characterize a QTL, qKW9, weight. This QTL encodes DYW motif pentatricopeptide repeat protein C-to-U editing ndhB,...
The anthocyanin biosynthesis of rice is a major concern due to the potential nutritional value. Purple appears in various organs and tissues such as pericarp, flower organs, leaves, leaf sheaths, internodes, ligules, apex, stigma. At present, there are many studies on color but gene mechanism other leaves still unclear, regulatory network specific organ coloring has not been systematically understood. In this study, genetic analysis demonstrated that purple traits were regulated by recessive...
Rice is one of the most important staple crops in world; therefore, improvement rice holds great significance for enhancing agricultural production and addressing food security challenges. Although there have been numerous studies on role single-nucleotide polymorphisms (SNPs) with development next-generation sequencing technologies, research presence/absence variations (PAVs) limited. In particular, a scarcity exploring traits genes that may be affected by PAVs rice. Here, we extracted...
The brown planthopper (BPH) is a serious insect pest of rice and substantial threat to production. Identification new BPH resistance genes their transfer into modern cultivars are effective breeding approaches reduce the damage caused by BPH. In this study, we mapped gene 50-kb genomic interval between two InDel markers 4M03980 4M04041 on short arm chromosome 4 in indica cultivar BP60, where was Rathu Heenati Liu et al. (2015) named “Bph3”. This region contains annotated Os04g0201900...
Abstract Over the past 30 years, human disturbance and habitat fragmentation have severely endangered survival of common wild rice ( Oryza rufipogon Griff.) in China. A better understanding genetic structure O. populations will therefore be useful for development conservation strategies. We examined diversity natural at national, provincial, local levels using simple sequence repeat (SSR) markers. Twenty representative from sites across China showed high variability, approximately 44% total...
Low temperature is one of the important environmental factors that affect rice growth and yield. To better understand japonica responses to cold stress, isobaric tags for a relative absolute quantification (iTRAQ) labeling-based quantitative proteomics approach was used detected changes in protein levels. Two-week-old seedlings tolerant variety Kongyu131 were treated at 8°C 24, 48 72 h, then total proteins extracted from tissues analysis. A 5082 analysis, which 289 significantly regulated,...
摘要: 建立了食品中新型防腐剂曲酸的高效液相色谱-串联质谱的定量测定方法。动物禽肉、鱼虾甲壳类、酱菜类、水果蔬菜、面制品等固体样品经乙腈提取;酱及酱油、醋、酒、饮料、糖浆等液体样品经水稀释,乙酸锌和亚铁氰化钾沉淀蛋白;以C18柱为分离柱,流动相为乙腈和5 mmol/L乙酸铵甲酸溶液,采用电喷雾串联四极杆质谱进行检测。选择1个母离子和2个子离子进行选择反应监测,以13C6-曲酸作为内标,选择信号最强的子离子进行定量测定。固体类基质中的定量限(按信噪比(S/N)大于10计)为0.1 mg/kg;液体类基质中的定量限为2.5 mg/kg。在0.1~2.0 mg/L范围内呈现良好的线性关系,相关系数r>0.99。各种基质在3个添加水平的平均回收率在72.6%~114%之间,相对标准偏差均小于11.4%。本方法简单实用,准确可靠,适用范围包括了食品中可能使用曲酸这种食品添加剂的大部分基质,可以满足进出口食品中曲酸的定性和定量要求。
Persistent tapetal cell1 (PTC1) plays a curial role in pollen development, and is thought to function as transcriptional activator rice. However, the molecular mechanism of PTC1 regulating development its cis-elements are not well understood. We identified novel weak male sterility mutant (ms92) which exhibited expanded tapetum shrink grains. Map-based cloning allelic analysis suggested that ms92 was caused by DNA fragment substitution promoter PTC1. The decreased expression MS92/PTC1...
A method was established for the determination of deoxynivalenol (vomitoxin) in grain and its products based on solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The sample firstly extracted by acetonitrile-water (84:16, v/v). extract then cleaned-up an HLB solid phase cartridge. separation carried out a Phenomenex Kinetex C18 column (100 mm x4. 6 mm, 2.6 microm) gradient elution using 0.3% per hundred ammonia...
<p><em>The dust-depressors have been developed utilizing a method of microbial induced carbonate precipitation. This dust-depressor has the characteristics high efficiency for dust suppression and environmental protection. Optimal compositions urease studied. In addition, pure water, CaCl2 super absorbent polymer are chosen to compare with new on performances. The results showed that had 3.13 mm/min seepage velocity 79.1% efficiency, which were superior other performance...
Abstract Kernel weight is an important yield component in maize that was selected during domestication. Many kernel genes have been identified through mutant analysis, and are mostly involved the biogenesis functional maintenance of organelles or other fundamental cellular activities. However, only a limited number loci underlying quantitative variation cloned. Here we characterize QTL, qKW9 find it encodes DYW motif pentatricopeptide repeat protein C-to-U editing NdhB, subunit chloroplast...