A5100377124- Atrial Fibrillation Management and Outcomes
- Venous Thromboembolism Diagnosis and Management
- Blood Pressure and Hypertension Studies
- Vitamin D Research Studies
- Meta-analysis and systematic reviews
- Coffee research and impacts
- Blood Coagulation and Thrombosis Mechanisms
- Viral-associated cancers and disorders
- Metabolism, Diabetes, and Cancer
- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Parathyroid Disorders and Treatments
- Antibiotics Pharmacokinetics and Efficacy
- S100 Proteins and Annexins
- Advanced Proteomics Techniques and Applications
- Smoking Behavior and Cessation
- Thyroid Cancer Diagnosis and Treatment
- Prostate Cancer Treatment and Research
- Salivary Gland Disorders and Functions
- Lipid metabolism and disorders
- Dermatologic Treatments and Research
- Cytomegalovirus and herpesvirus research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Analytical Chemistry and Sensors
- Renal cell carcinoma treatment
Wenzhou Vocational College of Science and Technology
2025
Beijing Hospital
2009-2024
Weatherford College
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2020-2024
State Council of the People's Republic of China
2023
Baoding People's Hospital
2022
National Center for Clinical Laboratories
2019-2021
Oxidative stress (OS) and reduced nitric oxide (NO) bioavailability contribute to the pathogenesis of pulmonary hypertension (PH). Whether there are associations between OS NO signaling biomarkers whether these associated with severity PH remain unclear. Blood samples were collected from 35 healthy controls patients arterial (PAH, n = 12) or chronic thromboembolic (CTEPH, 23). The mean artery pressure (mPAP) vascular resistance index (PVRI) measured by right heart catheterization. We...
Pseudorabies virus (PRV) heavily depends on host machinery to support its life cycle. Investigating the interaction between PRV and could aid in understandings of viral pathogenesis. In this study, we performed a 4D label free proteomic method examine differentially expressed proteins porcine kidney PK-15 cells with infection. The results showed that levels 661 were significantly elevated 693 markedly reduced. Furthermore, these altered primarily enriched spliceosome, protein processing...
BACKGROUND The aim of this study was to evaluate the role urinary sarcosine/creatinine ratio in diagnosis and prognosis prostate cancer, using a sarcosine oxidase assay. MATERIAL AND METHODS Urine samples were obtained from 203 patients with benign hyperplasia (BPH) 209 cancer. Levels measured method. ratios compared between group BPH In two groups, measurement serum prostate-specific antigen (PSA) free/total (F/T) PSA ratio. Correlations Gleason grade stage cancer analyzed. RESULTS There...
Purpose Investigations of the molecular mechanisms underlying metastatic phenotype papillary thyroid cancer (PTC) and identification novel candidate biomarkers to better predict PTC metastasis are urgently required. Experimental Design Tandem mass tag‐based quantitative proteomics approach is used identify differentially expressed proteins (DEPs) in tumorous tissues with different degrees lymph node metastases (LNMs). Furthermore, DEPs their clinical significance analyzed another independent...
Qichen Long, M.D., Tianqi Qi, Tianjiao Zhang, B.D., Jing Wang, Jie Zeng, Ying Yan, Meng Wei Huang, Haijian Zhao, Wenxiang Chen, Ph.D., Chuanbao M.D.. Ann Lab Med 2021;41:68-76. https://doi.org/10.3343/alm.2021.41.1.68
Osteoporosis is a worldwide disease that seriously affects the quality of life and survival rate elderly. The detection bone biomarkers will provide supplementary information on mineral density, contributing to accurate diagnosis osteoporosis better health care for prevention. This study aimed investigate efficacy oxidative stress markers-8-oxo-7,8-dihydroguanine (8-oxoGsn) 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGsn) in assessment osteoporosis. We conducted cross-sectional among...
In this study, we developed a method for determining cotinine and 3-hydroxycotinine in human serum established methodology an in-depth study of tobacco exposure health. After the proteins samples were precipitated with acetonitrile, they separated on ZORBAX SB-Phenyl column mobile phase methanol encompassing 0.3% formic acid-water 0.15% acid. The measurement was performed API5500 triple quadrupole mass spectrometer multiple reaction monitoring mode. Cotinine, 3-hydroxycotinine, cotinine-d3...
Abstract Background Mismatch repair deficiency (dMMR) status induced by MLH1 protein plays a pivotal role in therapeutic decision‐making for cancer patients. Appropriate quality control (QC) materials are necessary monitoring the accuracy of assays used clinical laboratories. Methods CRISPR/Cas9 technology was to edit gene GM12878Cas9 cells establish protein‐deficient cell lines. The positive lines were screened and validated Sanger sequencing, Western blot (WB), next‐generation sequencing...
Abstract Objectives This study aims to evaluate the safety of radiofrequency device and its efficacy in various treatment refrigeration modes. Methods Four 4‐week Bama miniature pigs were used this study, four repeated sites selected on pig's abdomen, each site consisting 6 different cooling modes, with (YouMagic; WE Medical Technology Co., Ltd.) administered every 3–5 s for a total five treatments. The handheld infrared thermometer (HIKMICRO; Hangzhou Hikmicro Sensing was monitor surface...
Current laboratory examinations for hypercoagulable diseases focus on the biomarker content of activated coagulation cascade and fibrinolytic system. Direct detection physiologically important protease activities in blood remains a challenge. This study aims to develop general approach that enables determination crucial factors plasmin blood. assay is based proteolytic activation an engineered zymogen l-phenylalanine oxidase (proPAO), which specific cleavage sites were between inhibitory...
Objective To establish sex- and age-specific reference intervals (RI) for adult serum immunoglobulin G (IgG) by two indirect methods. Methods Serum IgG detection results of all patients in Beijing Hospital from 2020 to 2021 were collected. After data deletion, removal duplicates outliers, the Hoffmann Bhattacharya methods used analysis, RI total population, different genders, ages seasons (months) calculated. The obtained compared with manufacturers National industry standards China, so as...
Objective To evaluate the analytical performance of new detection method for whole range CRP. Methods The linearity, limit detection, precision and interference were evaluated. correlation bias analyzed as compared with high sensitive CRP reagent. reference was defined. Results linearity 0.080-355.950 mg/L 0. 057 imprecision intra-assay 4. 12% (0.34 ), 2. 88% ( 13.08 mg/L), 1.94% (84.43 mg/L). total 8. 82% 3. 29% (13.08 2.61% recovery ranged 99.00% (0.100 100.36% (2.750 99.27%(8.270 101.19%...
Objective To investigate serum vitamin D levels and influencing factors in elderly patients with stage 3 to 4 chronic kidney disease (CKD) analyze the relationship between deficiency left ventricular hypertrophy (LVH). Methods This study involved 107 outpatients (≥60 years) CKD who had visited Beijing Hospital January 2014 September 2016.We retrospectively analyzed their clinical laboratory data including gender, age, height, weight, blood pressure, glycosylated hemoglobin, hemoglobin...
Objective To investigate the clinical features of elderly patients with stage 3-4 chronic kidney disease (CKD) and to analyze risk factors function progression. Methods This was a cross-sectional study.The clinic data (≥60 years) stable manifestation in Beijing Hospital from January, 2014 December, 2015 collected.Based upon eGFR derived Cockcroft-Gault (CG) equation, all were divided into groups CKD 3a group, 3b group 4 respectively.Moreover, separated old (< 80 very (≥80 based on...
Objective To investigate serum vitamin D levels in tuberculosis (TB) patients with different blood glucose status. Methods Two hundred and forty seven TB were recruited from clinics Jilin province 80 normal subjects who underwent health check up Beijing Hospital served as controls. Blood samples collected, fasting (FBG) [25(OH)D] measured. Results FBG results showed that there 95 FBG, 69 pre-diabetes (pre-DM) 83 diabetes mellitus (DM). Vitamin measurement 25(OH)D level pre-DM DM...
Objective To investigate the distribution characteristics of nasal cavity and maxillary sinus microbiota in aged patients with chronic sinusitis. Methods A total 15 unilateral sinusitis who received surgical treatment between January 2017 to June 2018 Beijing Hospital were enrolled analyzed retrospectively.Their lavage samples from cavity(N)and sinus(M)were collected labeled according location(N M groups, n=15 each). The high-throughput sequencing was used for all bacterial 16S rRNA...