Similar but different: A benzothiazole derivative can be used to detect cysteine (Cys) and homocysteine (Hcy) simultaneously in neutral media. The method involves thioether formation followed by cyclization release 2-(2′-hydroxy-3′-methoxyphenyl)benzothiazole (HMBT) a lactam. differences ring-formation kinetics allow spectral or kinetic modes identify Cys Hcy. Detailed facts of importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, not...

Many studies have shown that glutathione (GSH) and cysteine (Cys)/homocysteine (Hcy) levels are interrelated in biological systems. To unravel the complicated biomedical mechanisms by which GSH Cys/Hcy involved various disease states, probes display distinct signals response to highly desirable. In this work, we report a rhodol thioester (1) responds with fluorescence emissions neutral media. Probe 1 reacts form corresponding deconjugated spirolactam via tandem native chemical ligation (NCL)...

Spirocyclization of xanthene dyes has become a powerful technique for developing fluorescent probes. Herein, we extend this unique fluorescence switching mechanism to near-infrared (NIR) dye, 2-(7-diethylamino-2-oxo-2H-1-benzopyran-3-yl)-4-(2-carboxyphenyl)-7-diethylamino-1-benzopyrylium (CB), and construct ratiometric probe 1 cysteine (Cys)/homocysteine (Hcy). The sensing toward Cys/Hcy is realized by utilizing tandem native chemical ligation/spirocyclization reaction interrupt the large...

Selenocysteine (Sec) carries out the majority of functions various Se-containing species in vivo. Thus, it is great importance to develop sensitive and selective assays detect Sec. Herein, a carbon-dot-based fluorescent turn-on probe for highly detection selenol living cells presented. The photoluminescent carbon dots that emit yellow-green fluorescence (Y-G-CDs; λmax = 520 nm water) were prepared by using m-aminophenol as precursor through facile solvothermal method. surface Y-G-CDs was...

A fluorescent chemodosimeter for cysteine detection was developed based on a tandem conjugate addition and intramolecular cyclization reaction. The method exhibited an excellent selectivity over other biothiols such as homocysteine glutathione.

A laser induced breakdown spectroscopy (LIBS) technique coupled with SVM and PLS was proposed to perform quantitative classification analysis of 20 slag samples.

LIBS combined with random forest regression (RFR) was proposed for the quantitative analysis of multiple elements in fourteen steel samples.

Hydrogen sulfide (H2S) is a vital endogenous signal molecule that exerts critical physiological functions such as biological regulation and cytoprotection. Despite significant progress in developing H2S donors, site-specific delivery controllable release of systems remain key challenge. Herein, we develop new Cys-triggered fluorescent donor Pro-S composed dicyanoisophorone-based near-infrared (NIR) dye thiocarbamate moiety. The releases under the attack Cys, accompanied by reporter, which...

Early diagnosis of rheumatoid arthritis (RA) is crucial to prevent deterioration and improve the prognosis disease outcome. However, current clinical diagnostic methods are unable achieve accurate early detection RA. In this work, we designed an activatable organic nanoprobe (ONP-CySe) capable specific real-time imaging ClO– in ONP-CySe comprises a near-infrared fluorescent selenomorpholine-caged cyanine dye as sensing component amphiphilic triblock copolymer triphenyl phosphine derivative...

Cysteine S-sulfinylation is an important oxidative post-translational modification on proteins that regulates a variety of cell and protein functions. Aberrant contributes to range human pathologies, such as cancer cardiovascular disease. However, efforts elucidate the roles in physiology disease have been hampered due lack methodology for selective direct monitoring this living cells. In work, we developed fluorogenic probe PAK sulfinic acids by using 1,2-allenic ketone recognition moiety....

A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) homocysteine (Hcy). The proposed is precise with relative standard deviation (R.S.D) lower than 6% (n = 3) has been successfully applied to determine in human plasma...

Abstract A hybrid coumarin–thiazole compound was developed as a novel ratiometric and colorimetric sensor for bisulfite anions. Structure identification of the confirmed by 1 H NMR, 13 C H, COSY, heteronuclear single quantum coherence (HSQC), IR, HRMS spectroscopy. The detection anions performed through Michael addition anion toward sensor. reaction between caused fluorescence intensity to decrease at 600 nm increase 450 simultaneously yielded visible color change from purplish red colorless...

Acrylate has been widely used as the recognition unit for Cys fluorescent probes. Despite this widespread use, a potential drawback of probe type is that ester linkage between fluorophore and acryloyl liable to be hydrolyzed by abundant esterase in cytosol, thus affording high background signal. To solve problem, we herein put forward new strategy construct selective cysteine (Cys)/homocysteine (Hcy) with propynamide moiety. The free CPA displays weakly emission aqueous media because...

The hydroxyl radical (·OH), one of the reactive oxygen species (ROS) in biosystems, is found to be involved many physiological and pathological processes. However, specifically detecting endogenous ·OH remains an outstanding challenge owing high reactivity short lifetime this radical. Herein, inspired by scavenging mechanism a neuroprotective drug edaravone toward ·OH, we developed new ·OH-specific fluorescent probe RH-EDA. RH-EDA hybrid rhodamine exploits 3-methyl-pyrazolone moiety control...

Ähnlich, doch nicht gleich: Ein Benzothiazolderivat kann Cystein (Cys) und Homocystein (Hcy) parallel in neutralen Medien nachweisen. Bei dieser Methode wird zunächst ein Thioether gebildet, bevor eine Lactamcyclisierung unter Freisetzung von 2-(2′-Hydroxy-3′-methoxyphenyl)benzothiazol (HMBT) erfolgt. Der unterschiedlich schnelle Ringschluss ermöglicht die Identifizierung Cys Hcy auf spektralem oder kinetischem Weg. Detailed facts of importance to specialist readers are published as...