INI1/hSNF5 is a component of the ATP-dependent chromatin remodeling hSWI/SNF complex and tumor suppressor gene aggressive pediatric atypical teratoid malignant rhabdoid tumors (AT/RT). To understand molecular mechanisms underlying its function, we studied effect reintroduction into AT/RT-derived cell lines such as MON that carry biallelic deletions locus. We demonstrate expression causes G(0)-G(1) arrest flat formation in these cells. In addition, repressed transcription cyclin D1 MON,...

Eleven patients with moderate to severe erectile dysfunction (ED) were given a single-dose corpus cavernosum injection of hMaxi-K, "naked" DNA plasmid carrying the human cDNA encoding hSlo (for slowpoke), gene for α, or pore-forming, subunit smooth muscle Maxi-K channel. Three each 500, 1000, and 5000 µg, two 7500 hMaxi-K monitored 24 weeks. The primary objectives this phase I study safety tolerability escalating doses as assessed by clinical evaluations laboratory tests. Secondary efficacy...

Staphylococcus aureus is frequently isolated in the setting of infections indwelling medical devices, which are mediated by microbe's ability to form biofilms on a variety surfaces. Biofilm-embedded bacteria more resistant antimicrobial agents than their planktonic counterparts and often cause chronic sepsis, particularly patients with prolonged hospitalizations. In this study, we demonstrate that sustained nitric oxide-releasing nanoparticles (NO-np) interfere S. adhesion prevent biofilm...

The ability of gene transfer with the pore-forming subunit human maxi-K channel (hSlo) to ameliorate decline in erectile capacity commensurate 12-24 wk streptozotocin (STZ)-diabetes was examined 181 Fischer-344 rats. A 2-mo period STZ-diabetes induced before transfer, and evaluated by measuring intracavernous pressure response (ICP) cavernous nerve (CN) stimulation (ranging from 0.5 10 mA). In first series experiments, ANOVA revealed increased CN-stimulated ICP responses at 1 2 mo...

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Jul 2003The Successful Long-Term Treatment Age Related Erectile Dysfunction with hSlo cDNA in Rats In Vivo A. MELMAN, W. ZHAO, K.P. DAVIES, R. BAKAL, and G.J. CHRIST MELMANA. MELMAN , ZHAOW. ZHAO DAVIESK.P. DAVIES BAKALR. BAKAL CHRISTG.J. View All Author Informationhttps://doi.org/10.1097/01.ju.0000063375.12512.6eAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail...

HIV-1 integrase (IN) is a virally encoded protein required for integration of viral cDNA into host chromosomes. INI1/hSNF5 component the SWI/SNF complex that interacts with IN, selectively incorporated (but not other retroviral) virions, and modulates multiple steps, including particle production infectivity. To gain further insight role INI1 in replication, we screened INI1-interacting proteins using yeast two-hybrid system. We found SAP18 (Sin3a associated 18 kD), Sin3a-HDAC1 complex,...

ABSTRACT Candida albicans is a leading nosocomial pathogen. Today, candidal biofilms are significant cause of catheter infections, and such infections becoming increasingly responsible for the failure medical-implanted devices. C. forms in which fungal cells encased an autoproduced extracellular polysaccharide matrix. Consequently, enclosed fungi protected from antimicrobial agents host cells, providing unique niche conducive to robust microbial growth harbor recurring infections. Here we...

To determine if ProL1, a member of the opiorphin family genes, can modulate erectile physiology, as it encodes peptide which acts neutral endopeptidase inhibitor, other examples (Vcsa1, hSMR3A) physiology.We cloned members genes into same mammalian expression backbone (pVAX); 100 microg these plasmids (pVAX-Vcsa1, -hSMR3A, -hSMR3B and -ProL1) were injected intracorporally retired breeder rats affect on physiology assessed visually, by histology measuring intracavernous pressure (ICP) blood...

Intracorporal injection of plasmids encoding opiorphins into retired breeder rats can result in animals developing a priapic-like condition. Microarray analysis demonstrated that following intracorporal gene transfer expressing the most significantly upregulated corporal tissue was ornithine decarboxylase (ODC). Quantitative RT-PCR confirmed upregulation ODC, as well other genes involved polyamine synthesis, such arginase-I and -II, oxidase, spermidine synthase, acetyltransferase (SAT),...

Abstract Aims Two phase 1 trials were performed in healthy women with the overactive bladder (OAB) syndrome and urodynamically demonstrated detrusor overactivity (DO), aim to demonstrate safety potential efficacy of URO‐902, which comprises a gene therapy plasmid vector expressing human big potassium channel α subunit. Methods ION‐02 (intravesical instillation) ION‐03 (direct injection) double‐blind, placebo‐controlled, multicenter studies without overlap enrollment between studies. Active...

The paradigm of antigenic variation in parasites is the variant surface glycoprotein (VSG) African trypanosomes. Only one VSG expressed at any time, except for short periods during switching. reasons this pattern expression and consequences expressing more than are unknown. Trypanosoma brucei was genetically manipulated to generate cell lines that two VSGs simultaneously. These were produced equal amounts homogeneously distributed on trypanosome surface. double-expressor cells had similar...

To determine if the mature peptide product of Vcsa1 gene, sialorphin, could restore erectile function in ageing rats, and whether these effects are mediated through relaxation corporal smooth muscle tissue, as we recently reported that is one most down-regulated genes corpora rats three distinct models dysfunction, gene transfer plasmids expressing into restored function.Sialorphin was injected intracorporeally retired breeder effect on physiology tissue analysed by intracorporal/blood...

Protein expression profiles in rat bladder smooth muscle were compared between animal models of streptozotocin-induced diabetes mellitus (STZ-DM) and age-matched controls at 1 week 2 months after induction hyperglycemia with STZ treatment. At each time point, protein samples from four STZ-DM control tissues prepared independently analyzed together across multiple DIGE gels using a pooled internal standard sample to quantify changes statistical confidence. A total 100 spots determined be...

Protein expression profiles in rat corporal smooth muscle tissue were compared between animal models of streptozotocin-induced diabetes mellitus (STZ-DM) and age-matched controls (AMCs) at 1 week 2 months after induction hyperglycemia with STZ treatment. At each time point, protein samples from four STZ-DM AMC corpora tissues prepared independently analyzed together across multiple quantitative two-dimensional gels using a pooled internal standard sample to quantify changes statistical...