A5051083721- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Tryptophan and brain disorders
- Neuroendocrine regulation and behavior
- Hormonal and reproductive studies
- Regulation of Appetite and Obesity
- Diet, Metabolism, and Disease
- Neuroscience and Neuropharmacology Research
- Stress Responses and Cortisol
- Neuropeptides and Animal Physiology
- Pharmacological Effects and Assays
- Peptidase Inhibition and Analysis
- Pharmacology and Obesity Treatment
- Pain Mechanisms and Treatments
- Acute Lymphoblastic Leukemia research
- Psychological Treatments and Assessments
- Diabetes Treatment and Management
- Amino Acid Enzymes and Metabolism
- Cardiovascular Disease and Adiposity
- Adenosine and Purinergic Signaling
- Schizophrenia research and treatment
- Prenatal Substance Exposure Effects
- Childhood Cancer Survivors' Quality of Life
Rudjer Boskovic Institute
2012-2024
Institute of Neurobiology
1998
The thymus is the central hub of T-cell differentiation, where epithelial cells guide process their maturation. Our goal was to identify and describe progenitor within human that can differentiate into cells. When we plated enriched thymic in 3D culture conditions, rare individual capable self-renewal differentiation formed spheroids. Both neonatal adult thymuses produced similar numbers spheroids, suggesting potential remains consistent across age groups. Some spheres express genes typical...
Reduced peripheral serotonin (5HT) in mice lacking tryptophan hydroxylase (TPH1), the rate limiting enzyme for 5HT synthesis, was reported to be anabolic skeleton. However, other studies TPH1 deletion either had no bone effect or an age dependent inhibition of osteoclastic resorption. The role therefore remains poorly understood. To address this issue, we used selective breeding create rat sublines with constitutively high (high-5HT) and low (low-5HT) platelet level (PSL) uptake (PSU)....
We examined the relationship between density of serotonergic (5-hydroxytryptamine [5-HT]) uptake sites and extracellular 5-HT concentration in rat brain using microdialysis with two different models, lesions 5,7-dihydroxytryptamine (50 microg dorsal raphe nucleus (DRN) 15 days before) sublines rats genetically selected displaying extreme values platelet uptake. Compared to controls, lesioned had a reduced cortical 5-hydroxyindoles (45%), unchanged basal DRN ventral hippocampus (VHPC),...
Central and peripheral pools of biogenic monoamine serotonin (5-hydroxytryptamine, 5HT) exert opposite effects on the body weight regulation: increasing brain 5HT activity is expected to decrease weight, while will increase adiposity. In a genetic model rats with constitutionally high or low homeostasis (hyper/hyposerotonergic rats), we have studied how individual differences in endogenous tone modulates net energy balance organism. The high-5HT low-5HT sublines were developed by selective...
<i>Objective:</i> The effect of serotonin (5-hydroxytryptamine; 5HT) on the in vitro proliferation mitogen-stimulated lymphocytes was studied primary cultures rat spleen cells. <i>Methods:</i> 5HT added to 1 h prior mitogen, at final concentrations from 10<sup>–13</sup> up 10<sup>–2</sup><i>M</i>. T and B cell mitogens (concanavalin A, pokeweed mitogen lipopolysaccharide) were used suboptimal optimal concentrations. measured 24–72...
Serotonin (5-HT) plays an extensive role during pregnancy in regulating both the placental physiology and embryonic/fetal development. The uptake of 5-HT into cells is central to control local concentrations near its molecular targets. Here, we investigated mechanisms human primary cord blood platelets, all isolated immediately after birth. Trophoblasts platelets showed with similar Michaelis constant (Km) values (~0.6 μM), typical high-affinity serotonin transporter (SERT). trophoblasts was...
Chronic lymphocytic leukemia (CLL) is a lymphoproliferative disorder characterized by specific expansion of mature B-cell clones. We hypothesized that the disease has heterogeneous clinical outcome depends on genes and signaling pathways active in malignant clone individual patient. It was found several are CLL, namely, NOTCH1, Ikaros family genes, BCL2, NF-κB, all which contribute to cell survival proliferation leukemic clone. Therefore, we analyzed primary CLL cells for gene protein...
A role of the serotonin (5HT) transporter, a key regulator serotonergic transmission, in physiology, pharmacology and genetics pain responses has been proposed recently. The present study aimed to explore impact constitutive differences activity 5HT homeostasis general, on modulation sensitivity analgesic drugs that utilize mechanisms.A novel genetic animal model, Wistar-Zagreb rats, obtained by selective breeding animals for extreme platelet transporter was used. As consequence breeding,...