A5006721537- COVID-19 epidemiological studies
- Infection Control and Ventilation
- Viral Infections and Outbreaks Research
- COVID-19 impact on air quality
- SARS-CoV-2 and COVID-19 Research
- Disaster Response and Management
- Viral gastroenteritis research and epidemiology
- Chemical Looping and Thermochemical Processes
- COVID-19 and healthcare impacts
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Viral Infections and Vectors
- Photoacoustic and Ultrasonic Imaging
- Indoor Air Quality and Microbial Exposure
- Bacteriophages and microbial interactions
- Plant and Fungal Interactions Research
- Influenza Virus Research Studies
- Burkholderia infections and melioidosis
- Respiratory viral infections research
- Coagulation and Flocculation Studies
- Calibration and Measurement Techniques
United States Department of Homeland Security
2019-2022
Battelle
2020-2022
George Mason University
2021
Defence Science and Technology Laboratory
2017
Gojo (United States)
2017
CollegeAmerica
2017
Scientific Consulting Group
2017
Aerosols represent a potential transmission route of COVID-19. This study examined effect simulated sunlight, relative humidity, and suspension matrix on stability SARS-CoV-2 in aerosols. Simulated sunlight significantly affected decay rate the virus. Relative humidity alone did not affect rate; however, minor interactions between other factors were observed. Mean rates (± SD) saliva, under levels representative late winter/early fall summer 0.121 ± 0.017 min-1 (90% loss, 19 minutes) 0.306...
Recent evidence suggests that respiratory aerosols may play a role in the spread of SARS-CoV-2 during ongoing COVID-19 pandemic. Our laboratory has previously demonstrated simulated sunlight inactivated and on surfaces. In present study, we extend these findings to include persistence across range temperature, humidity, levels using an environmentally controlled rotating drum aerosol chamber. The results demonstrate sunlight, humidity are all significant factors influencing infectious...
Abstract Background During a period of rapid growth in our understanding the microbiology built environment recent years, majority research has focused on bacteria and fungi. Viruses, while probably as numerous, have received less attention. In response, Alfred P. Sloan Foundation supported workshop entitled “Viruses Built Environment (VIBE),” at which experts environmental engineering, microbiology, epidemiology, infection prevention, fluid dynamics, occupational health, metagenomics,...
Environmental parameters, including sunlight levels, are known to affect the survival of many microorganisms in aerosols. However, impact on influenza virus aerosols has not been previously quantified.The present study examined influence simulated at both 20% and 70% relative humidity using an environmentally controlled rotating drum aerosol chamber.Measured decay rates were dependent level sunlight, but they significantly different between 2 levels tested. In darkness, average constant was...
Numerous studies have demonstrated that SARS-CoV-2 can be inactivated by ultraviolet (UV) radiation. However, there are few data available on the relative efficacy of different wavelengths UV radiation and visible light, which complicates assessments decontamination interventions. The present study evaluated effects monochromatic at 16 from 222 nm through 488 in liquid aliquots dried droplets water simulated saliva. were used to generate a set action spectra quantify susceptibility genome...
Abstract Background Our laboratory previously examined the influence of environmental conditions on stability an early isolate SARS-CoV-2 (hCoV-19/USA/WA-1/2020) in aerosols generated from culture medium or simulated saliva. However, genetic differences have emerged among lineages, and it is possible that these may affect potential for aerosol transmission. Methods The temperature, relative humidity, sunlight decay 4 isolates aerosols, including 1 belonging to recently B.1.1.7 lineage, were...
In support of the response to 2013–2016 Ebola virus disease (EVD) outbreak in Western Africa, we investigated persistence virus/H.sapiens-tc/GIN/2014/Makona-C05 (EBOV/Mak-C05) on non-porous surfaces that are representative hospitals, airplanes, and personal protective equipment. We performed studies three clinically-relevant human fluid matrices (blood, simulated vomit, feces), at environments in-flight airline passenger cabins, environmentally-controlled hospital rooms, open-air treatment...
Ebola virus (EBOV) in body fluids poses risk for transmission. However, there are limited experimental data such matrices on the disinfectant efficacy against EBOV. We evaluated effectiveness of disinfectants EBOV blood surfaces. Only 5% peracetic acid consistently reduced titers dried to assay limit quantification.
Previous laboratory studies of Ebola virus aerosols have used a variety different sampling devices to collect viral material for analysis. However, vary in their ability aerosol particles and preserve the infectivity collected viruses. If not accounted for, this variability has potential bias study outcomes confound comparisons between which samplers are used. In present study, five laboratory-scale were assessed at low high relative humidities. Relative humidity did affect physical...
Previous field and laboratory studies investigating airborne Burkholderia pseudomallei have used a variety of different aerosol samplers to detect quantify concentrations the bacteria in aerosols. However, performance can vary their ability preserve viability collected microorganisms, depending on resistance organisms impaction, desiccation, or other stresses associated with sampling process. Consequently, sampler selection is critical maximizing probability detecting viable microorganisms...
A variety of methods have been developed for quantification infectious Ebola virus in clinical or laboratory samples, but existing often require extensive operator involvement, manual assay scoring, the use custom reagents. In this study, we utilize a recently Ebola-specific reporter cell line that expresses ZsGreen response to infection, conjunction with semi-automated processing and techniques, develop an unbiased, high-throughput microtitration vitro. This was found equivalent sensitivity...
As the COVID-19 pandemic was overtaking world in spring of 2020, National Institute Standards and Technology (NIST) began collaborating with Biodefense Analysis Countermeasures Center to study inactivation SARS-CoV-2 after exposure different ultraviolet (UV) blue light wavelengths. This paper describes a 1 kHz pulsed laser projection system used doses required inactive over wavelength range 222 488 nm. builds on NIST's previous work for water pathogen using UV irradiation. The design its...