A5070275930- Spinal Cord Injury Research
- Bone Metabolism and Diseases
- Cerebral Palsy and Movement Disorders
- Bone health and treatments
- Bone and Joint Diseases
- Pain Mechanisms and Treatments
- Musculoskeletal pain and rehabilitation
- Stroke Rehabilitation and Recovery
- Bone fractures and treatments
- Bone health and osteoporosis research
- Oral microbiology and periodontitis research
- Hip and Femur Fractures
- Acupuncture Treatment Research Studies
- Nerve injury and regeneration
- Osteoarthritis Treatment and Mechanisms
- Endodontics and Root Canal Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Trace Elements in Health
- Mesenchymal stem cell research
- Traumatic Brain Injury Research
- Nerve Injury and Rehabilitation
- Diabetic Foot Ulcer Assessment and Management
- Spine and Intervertebral Disc Pathology
- Dental materials and restorations
- S100 Proteins and Annexins
University of Minnesota
2019-2024
University of Miami
2024
University of Minnesota System
2021-2024
University of Minnesota Medical Center
2019-2023
University of Colorado Denver
2016-2019
Institute of Cardiology
2018
University of Colorado Anschutz Medical Campus
2017-2018
University of Colorado Boulder
2017
University of Colorado System
2017
Harvard University
2008-2015
5-HTT mediates antidepressant-sensitive clearance of 5-HT after its release into neural synapses. We found increased expression in RANKL-induced osteoclast-like cells. Fluoxetine, an inhibitor 5-HTT, reduced osteoclast differentiation but not activation. Reserpine, intracellular transport, potentiated differentiation. These results indicate a role for function and suggest that commonly used antidepressive agents may affect bone mass.Interactions between the serotonergic skeletal systems are...
Abstract Spinal cord injury (SCI) results in profound bone loss due to muscle paralysis and the inability ambulate. Sclerostin, a Wnt signaling pathway antagonist produced by osteocytes, is potent inhibitor of formation. Short-term studies rodent models have shown increased sclerostin response mechanical unloading that reversed with reloading. These suggest complete spinal injury, condition resulting paralyzed lower extremities, will be associated high levels. We assessed relationship...
Abstract The role of the receptor activator nuclear factor κB (NF-κB) ligand (RANKL)—a tumor necrosis (TNF)-related cytokine—in osteoclast formation has been established clearly. However, downstream signaling pathways activated by this cytokine remain largely unknown. To identify genes that play a in osteoclastogenesis, we used RAW 264.7 mouse monocytes as model system for differentiation multinucleated osteoclasts from mononucleated precursors. cells were induced with RANKL to form giant...
Abstract Chemokines play an important role in immune and inflammatory responses by inducing migration adhesion of leukocytes, have also been reported to modulate osteoclast differentiation from hemopoietic precursor cells the monocyte-macrophage lineage. In this study, we examined effect MIP-1γ, a C-C chemokine family member, on receptor activator NF-κB ligand (RANKL)-stimulated differentiation, survival, activation. RANKL induced osteoclasts dramatically increase production MIP-1γ express...
Inhibition of dendritic cell maturation and activation, together with abnormal functioning cell-mediated immunity, has been reported in chronic spinal cord injury (SCI). The development immune-based therapies could: 1) prevent or slow down limit further tissue damage SCI, 2) promote regeneration. To identify novel candidate molecular pathways mediating SCI-induced immune changes, we performed whole-genome microarray pathway analyses. Subjects motor complete SCI (> 2 years post-injury)...
Abstract Mounting evidence exists for the operation of a functional serotonin (5‐HT) system in osteoclasts and osteoblasts, which involves both receptor activation 5‐HT reuptake. In previous work we showed that transporter (5‐HTT) is expressed its activity required by osteoclast differentiation vitro. The purpose current study was to determine effect treatment with fluoxetine, specific reuptake inhibitor, on bone metabolism vivo. Systemic administration fluoxetine Swiss–Webster mice 6 weeks...
RANKL-stimulation of osteoclast precursors results in up-regulation genes involved the process differentiation and activation. In this report we describe expression functional characterization Sorting Nexin 10 (snx10). Snx10 belongs to sorting nexin (SNX) family, a diverse group proteins with common feature: PX domain, which is membrane trafficking cargo endosomes. strongly up-regulated during RANKL-induced vitro expressed osteoclasts vivo. qPCR analysis confirmed significant increase snx10...
Cachexia is defined as an excessive, involuntary loss of fat and lean tissue. We tested the validity Lewis lung carcinoma (LLC) a model cancer cachexia examined its effect on two major tissue components, skeletal muscle bone. LLC cells (0.75 × 106) were injected into left thigh C57BL/6 mice. Control mice received equal volume injection growth media. Tumors observed in all LLC-injected animals 21 25 days post inoculation. showed significant reductions mass despite having same average daily...
Mutations in sorting nexin 10 (Snx10) have recently been found to account for roughly 4% of all human malignant osteopetrosis, some them fatal. To study the disease pathogenesis, we investigated expression Snx10 and created mouse models which was knocked down globally or out osteoclasts. Endocytosis is severely defective Snx10-deficent osteoclasts, as extracellular acidification, ruffled border formation, bone resorption. We also discovered that highly expressed stomach epithelium, with...
In the current concept of bacterial infections, pathogen-associated molecular patterns (PAMPs) derived from pathogens and damage-associated (DAMPs) released damaged/necrotic host cells are crucial factors in induction innate immune responses. However, implication DAMPs apical marginal periodontitis is unknown. Serum amyloid A (SAA) a DAMP that involved development various chronic inflammatory diseases, such as rheumatoid arthritis. present study, we tested whether SAA pathogenesis periapical...