Silver nanoparticles induced alterations in multiple cellular targets, which are critical for drug susceptibilities and pathogenicity fungal pathogen (Candida albicans) Venkatraman Srinivasan Radhakrishnan,1,2 Mohana Krishna Reddy Mudiam,3 Manish Kumar,1,2 Surya Prakash Dwivedi,4 Surinder Pal Singh,5 Tulika Prasad1,2 1Advanced Instrumentation Research Facility (AIRF), Jawaharlal Nehru University (JNU), New Delhi, India; 2Special Centre Nano Sciences (SCNS), 3Analytical Chemistry Lab, Council...

Abstract: Silver nanoparticles (AgNps) have attracted maximal attention among all metal nanoparticles, and the study of their biological properties has gained impetus for further medical adoption. This evaluated cellular molecular mechanisms associated with action AgNps against an opportunistic pathogen, Candida albicans . Spherical, stable AgNp (average size 21.6 nm) prepared by a chemical reduction method showed minimum inhibitory concentration (required to inhibit growth 90% organisms) at...

In the field of nanoparticle research, green synthesis approach is emerging as a promising avenue, particularly for medical applications. Achyranthes aspera, plant rich in bioactive compounds, has shown significant potential reduction silver and iron nanoparticles. The validation this process achieved through thorough examination using spectroscopy, scanning electron microscopy, FTIR, EDAX techniques. resulting nanoparticles exhibit distinctive properties that influence their stability...

The prevalence and mortality due to multifactorial polygenic diseases; hypertension, coronary artery disease (CAD), diabetes cancer vary depending upon genetic susceptibility environmental precursors because they have identifiable mendelian subsets.Rapid changes in diet lifestyle, may influence heritability of the variant phenotypes that are dependent on nutraceutical or functional food supplementation for their expression.It is possible recognize interaction specific nutraceuticals, with...

A new, simple, sensitive, selective, rapid, and high-throughput liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed validated for simultaneous quantification of Olmesartan hydrochlorothiazide in human plasma. Simple liquid-liquid extraction procedure was applied plasma sample pretreatment using a mixture diethyl ether dichloromethane, as an solution. Analytes were separated on UNISOL C18 150*4.6 mm, 5 µm column methanol, 2 mM ammonium acetate pH 5.5 (80:20,...

Citrus tristeza virus (CTV), a member of genus Closterovirus, is an important pathogen which has destroyed more than one million citrus trees in India till date. Survey was conducted commercial sp. covering all the growing geographical zones India. For detection CTV and estimation disease incidence, direct antigen coated- enzyme linked immuno-sorbent assay (DAC-ELISA), reverse transcription- polymerase chain reaction nucleotide sequence analyses were employed. The overall incidence 26.3%...

In this review, immune response and possible causes of depletion CD4 + T-cell counts in patients with human immunodeficiency (HIV)-1 infection, have been documented.HIV has recognized as a global problem; however, the developing countries are most affected by epidemic diseases.Countries sub-Saharan Africa seem to bear bulk HIV burden among about 24.7 million ( 63%) all people living globally 2006.The major factor obstructing progress towards an effective vaccine prevent or modulate -1...

Banners are present in several forms and a person might be inspired by one or more of these. However, designing banners is non-trivial task, especially for novices. Starting from blank canvas can often overwhelming, exploring alternatives time-consuming. In this paper, we propose an automatic approach to transfer novice user's content into example banner. Our algorithm begins with extracting the template banner via semantic segmentation approach. This followed energy-based optimization...

To identify the cases of trichomoniasis in symptomatic and asymptomatic Trichomonas vaginalis (T. vaginalis) infected patients by PCR amplification hypervariable 18S rRNA gene to assess sensitivity restriction fragment length polymorphism (RFLP) technique for their diagnosis. We enrolled 498 women child bearing age groups, with pre-informed consent, attending OPD routine checkups STI related problems. Trichomoniasis was diagnosed on basis wet mount preparations a primer set targeting...

Acyl CoA diacylglycerol acyltransferase (DGAT, EC 2.3.120) is recognized as a key player of cellular metabolism. It catalyzes the terminal, yet committed step in triacylglycerol synthesis using and fatty acyl substrates. The protein sequence acyltransferse (DGAT) Type 2B Moretierella ramanniana var. angulispora (Protein_ID = AAK84180.1) was retrieved from GenBank. However, structure not available for this sequence. 3D DGAT modeled template (PDB ID: 1K30) obtained Protein databank (PDB)...

Liver Alcohol dehydrogenase (LADH) catalyzes reversible oxidation of primary and secondary alcohols to aldehyde using NADH + as a coenzyme.The aim the present study was validate preexisting biophysical characterization erythritol simulation studies protein-erythritol-water interaction in solution at molecular level.Two dynamics simulations protein ADH room temperature were carried out, one presence (about 0.77 M) another absence erythritol.The molecules observed cluster move toward protein,...

Cryptosporidium parvum is the common enteric protozoan pathogen causing cryptosporidiosis in human. Available drugs to treat are ineffective and there yet no vaccine against C. parvum. Therefore, it of interest design an improved effective drug Here, we docked benzoxazole derivatives (collected from literature) with inosine 5`- monophosphate dehydrogenase (IMPDH) using program AutoDock 4.2. The protein - inhibitor complex structure was optimized molecular dynamics simulation for 5 ps...

The remarkable antibiotic properties of Achyranthes aspera leaf extracts have been harnessed in this study to produce iron nanoparticles (FeNPs) with potent antibacterial activity against various pathogens. Serving as both a reducing and capping agent, the phytochemical protein-rich composition A. leaves facilitated eco-friendly synthesis FeNPs. This delves into an innovative, environmentally conscious strategy for production by utilizing extract dual-function agent. resulting FeNPs, endowed...

Type II Diabetes Mellitus or Non-Insulin dependent mellitus (NIDDM) is one of the common diseases worldwide and often recognized as life style disease.The major symptom this disease an increase in blood glucose level resulting polydypsia, polyuria polyphagia.The cause abnormality either lack insulin production insensitivity to produced inside body.Glucagon like peptide (GLP) by glucagons gene responsible for secretion.Thus proper functioning GLP can be a remedy mellitus.DiPeptidyl Peptidase...

The availability of completely sequenced genomes allow the use computational techniques to investigate cis-acting sequences controlling transcription regulation associated with groups functionally related genes.Theoretical analysis was performed assign functions regulatory systems.The identification such sites is relevant for locating a promoter at 5' boundary gene.They also prediction specific gene-expression pattern and response disturbances in known signaling pathway.Here, we describe...

To develop an in-house PCR based diagnostic assay for identification of strains isolated from symptomatic and asymptomatic subjects India, targeting the β-tubulin gene using specific primers. In present study a primer set is designed to target well-conserved region in beta-tubulin Trichomonas vaginalis (T. vaginalis). All T. were tested successfully detected by yielding single predicted product 198 bp gel electrophoresis, while there was negative response with DNA Giardia lamblia, Toxoplasma...

Background and Objectives: Prevention of food spoilage is usually achieved by use chemical preservatives which have negative impacts human health.Due to such concerns it necessary find some natural preservatives.Within this text, the present study was designed investigate in vitro anti-microbial activities, MIC, MBC, phytochemical analysis anti-oxidant activity acetone extract bark Tamarindus indica against pathogenic micro-organisms.Materials Methods: The anti-bacterial anti-fungal...

Sugarcane grassy shoot diseases (SCGS) caused by phytoplasma is one of the major sugarcane which responsible for qualitative and quantitative losses. An extensive survey 13 districts central, western eastern Uttar Pradesh were done during 2013-2017 crops session. The disease incidence varied from 1-63% maximum was recorded in U P followed central UP. Total twenty four varieties observed most prominent symptoms white leaf, stunting. present study indicate wide occurrence SCGS almost all...