A5030405398- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Horticultural and Viticultural Research
- Plant-Microbe Interactions and Immunity
- Plant biochemistry and biosynthesis
- Phytoplasmas and Hemiptera pathogens
- Ubiquitin and proteasome pathways
- Plant Molecular Biology Research
- Cocoa and Sweet Potato Agronomy
- Plant tissue culture and regeneration
- Plant responses to water stress
- Plant Pathogens and Fungal Diseases
- Polysaccharides and Plant Cell Walls
- Plant and Fungal Interactions Research
- Powdery Mildew Fungal Diseases
- Biochemical and biochemical processes
- Ion Channels and Receptors
- Magnetic and Electromagnetic Effects
- Seed Germination and Physiology
- Light effects on plants
- Ecology and Conservation Studies
- Postharvest Quality and Shelf Life Management
- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
Ningxia University
2020-2025
Ningxia Academy of Agriculture and Forestry Sciences
2020-2023
Northwest A&F University
2012-2020
Ubiquitin-mediated regulation responds rapidly to specific stimuli; this rapidity is particularly important for defense responses pathogen attack. Here, we investigated the role of E3 ubiquitin ligase Erysiphe necator-induced RING finger protein 1 (EIRP1) in response Chinese wild grapevine Vitis pseudoreticulata. The regulatory function EIRP1 was using molecular, genetic and biochemical approaches. encodes a C3HC4-type Really Interesting New Gene (RING) that harbors activity. This activity...
Abstract Cold stress severely impacts the quality and yield of grapevine (Vitis L.). In this study, we extend our previous work to elucidate role regulatory mechanisms Vitis amurensis MYB transcription factor 4a (VaMYB4a) in grapevine's response cold stress. Our results identified VaMYB4a as a key positive regulator We demonstrated that undergoes phosphorylation by V. CBL-interacting protein kinase 18 (VaCIPK18) under stress, process activates transcriptional activity. Using ChIP-seq,...
Abstract Accumulation of stilbene phytoalexins stimulates resistance mechanisms against the grapevine fungus Uncinula necator . However, defensive triggered by synthase ( STS ) genes, remain largely unknown. Here, we report function and molecular mechanism gene VpSTS29/STS2 from Vitis pseudoreticulata in regulation plant responses to powdery mildew. Stilbene synthesis occurred mainly root tips mesophyll cells transgenic grapevines via transport through vascular bundles. Overexpression...
In order to compare and analyze the chloroplast (cp) genomes of tomato germplasms understand their phylogenetic relationships, cp 29 were sequenced analyzed in this study. The results showed highly conserved characteristics structure, number gene intron, inverted repeat regions, sequences among genomes. Moreover, single-nucleotide polymorphism (SNP) loci with high located at 17 fragments selected as candidate SNP markers for future studies. tree, tomatoes clustered into two major clades,...
This project aimed at breeding new seedless grape cultivars by embryo rescue through three hybridization methods: 1) using cross-breeding between Vitis vinifera and wild Chinese spp; 2) crossing with two cultivars, 3) grapes of different ploidy.Genotype, sampling times, media were confirmed to play important roles in this system.Among the genotypes, productions hybrid plants significantly different, ranging from 23.0% (Ruby Seedless x Black Olympia) only 1.1% (Pink Beichun), except for...
The ubiquitination system plays important roles in the degradation and modification of substrate proteins. In this study, we characterize a putative U-box type E3 ubiquitin ligase gene, VpPUB24 (plant protein 24), from Chinese wild grapevine, Vitis pseudoreticulata accession Baihe-35-1. We show that is induced by number stresses, especially cold treatment. Real-time PCR analysis indicated PUB24 transcripts were increased after stress different grapevine species, although relative expression...
Grapevine is one of the world's most important fruit crops. European cultivated grape species have best quality but show almost no resistance to powdery mildew (PM). PM caused by Uncinula necator a harmful disease that has significant impact on economic value crop. In this study, we examined RING-H2-type ubiquitin ligase gene VpRH2 associated with PM-resistance Chinese wild-growing Vitis pseudoreticulata accession Baihe-35-1. The expression was clearly induced U. inoculation compared its...
GATA transcription factors are type IV zinc-finger proteins that play key roles in plant growth and responses to environmental stimuli. Although these have been studied model plants, the related studies of gene family under abiotic stresses rarely reported grapevine (Vitis vinifera L.).In current study, a total 23 VviGATA genes were identified classified into four groups (I, II, III, IV), based on phylogenetic analysis. The same group exhibited similar exon-intron structures conserved motifs...
Abstract Background Wolfberry is well-known for its high nutritional value and medicinal benefits. Due to the continuous ripening nature of Goji berries fact that they can be commercially harvested within a few weeks, their phytochemical composition may change during harvesting process at different periods. Result The involved molecular mechanisms difference in fruit quality ripe Lycium barbarum L. four periods were investigated by transcriptomic metabolomics analyses first time. According...
In order to screen out the most stable reference genes in tomatoes under powdery mildew (PM) stress and study expression of related interaction between tomato PM more accurately, this will provide a calibration basis for functional genes. study, stabilities eight candidate EF1α, L33, Act, Ubi, GAPDH, UK, CAC TIP41 susceptible resistant were ranked using four different computation programs, including geNorm, Normfinder, BestKeeper comparative ∆CT method. Then RefFinder was used analyze...
The abnormal seedlings, a common physiological anomalies, emerged during embryo rescue severely restricted grape breeding. To enhance the efficiency of seedless breeding by reducing production seedlings in course rescue, we investigated effects genotype, media type, style, pre-chilling on deformity rate rescue. were firstly classified into seven categories based their morphology. Our results indicated that emergence was highly dependent female parent genotype. Polyembryony advantageous to...
Abstract Background RING is one of the largest E3 ubiquitin ligase families and C3H2C3 type subfamily RING, which plays an important role in plant growth development, responses to biotic abiotic stresses. Results A total 143 C3H2C3-type genes ( RCHCs ) were discovered from grapevine genome separated into groups (I-XI) according their phylogenetic analysis, these named positions on chromosomes. Gene replication analysis showed that tandem duplications play a predominant expansion VvRCHCs...
Powdery mildew is a serious problem in tomato production; therefore, the PM-resistant inbred line, '63187', and susceptible variety, 'Moneymaker (MM)', were used as experimental materials for combined analysis of transcriptome widely targeted metabolome on leaves at 0 h post inoculation (hpi), 12 hpi, 48 hpi. The results indicated that 276 genes expressed all treatments, K-means cluster showed these divided into eight classes '63187' ten 'MM'. KEGG enrichment amino acid metabolism, signal...