A5101671649- Cellular Mechanics and Interactions
- Protein Kinase Regulation and GTPase Signaling
- Echinoderm biology and ecology
- Immunodeficiency and Autoimmune Disorders
- Immune Cell Function and Interaction
- Marine Bivalve and Aquaculture Studies
- Toxoplasma gondii Research Studies
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Cell Adhesion Molecules Research
- Aquatic life and conservation
- Bone Tissue Engineering Materials
- Bone and Dental Protein Studies
- Public Health and Nutrition
- Plant responses to water stress
- Plant nutrient uptake and metabolism
- Mechanisms of cancer metastasis
- Plant Stress Responses and Tolerance
- Autophagy in Disease and Therapy
- Seaweed-derived Bioactive Compounds
- Polyamine Metabolism and Applications
- Force Microscopy Techniques and Applications
- Phytochemicals and Antioxidant Activities
- PI3K/AKT/mTOR signaling in cancer
- Polysaccharides and Plant Cell Walls
Iowa State University
2017-2024
Institute of Crop Sciences
2020
Chinese Academy of Agricultural Sciences
2020
The Rho GTPase Rac1 activates the WAVE regulatory complex (WRC) to drive Arp2/3 complex-mediated actin polymerization, which underpins diverse cellular processes. Here we report structure of a WRC-Rac1 determined by cryo-electron microscopy. Surprisingly, is not located at binding site on Sra1 subunit WRC previously identified mutagenesis and biochemical data. Rather, it binds distinct, conserved opposite end Sra1. Biophysical data mutants confirm that both sites, with newly having higher...
An inherited disorder makes WAVEs The WAVE regulatory complex (WRC) is a multiunit that regulates actin cytoskeleton formation. Although other actin-regulatory proteins modulate human immune responses, the precise role for WRC has not yet been established. Cook et al. studied five patients from four unrelated families who harbor missense variants of gene encoding component HEM1. These presented with recurrent infections and poor antibody along enhanced allergic autoimmune disorders. HEM1 was...
The Rho-family GTPase Rac1 activates the WAVE regulatory complex (WRC) to drive Arp2/3 complex-mediated actin polymerization in many essential processes. binds WRC at two distinct sites-the A and D sites. Precisely how binding triggers activation remain unknown. Here we report structures by itself, when bound single or double molecules, ~3 Å resolutions cryogenic-electron microscopy. reveal that sites mechanisms, site, but not drives activation. Activation involves a series of unique...
High soil salinity can cause significant losses in rice productivity worldwide, mainly because salt inhibits plant growth and reduces grain yield. To cope with environmental changes, plants have evolved several adaptive mechanisms that involve the regulation of many stress-responsive genes.
Cross-talk between Rho- and Arf-family guanosine triphosphatases (GTPases) plays an important role in linking the actin cytoskeleton to membrane protrusions, organelle morphology, vesicle trafficking. The central regulator, WAVE regulatory complex (WRC), integrates Rac1 (a Rho-family GTPase) Arf signaling promote Arp2/3-mediated polymerization many processes, but how WRC senses is unknown. Here, we have reconstituted a direct interaction WRC. This greatly enhanced by binding D site of Arf1...
This study aimed to examine the combined use of bone morphogenetic protein-2 (BMP-2) and polysaccharide isolated from Stichopus japonicus on osteogenic differentiation MC3T3-E1 cells. Osteogenic was measured via histochemical staining alkaline phosphatase (ALP) assay, alizarin red mineralization Western blotting, ELISA, a qRT-PCR evaluation for expression BMP-2, runt-related transcription factor-2 (Runx-2), osteocalcin (OCN), osteopontin (OPN), collagen type I (Col I) in Immunofluorescence...
Abstract Branched actin networks polymerized by the Arp2/3 complex are critical for cell migration. The WAVE is machinery that activates in a RAC1-dependent manner at leading edge of migrating cells. Multiple complexes assembled through various combinations paralogous subunits. Here we report surprising phenotype associated with loss-of-function CYFIP2, subunit complex. In three different human mammary lines and prechordal plate cells gastrulating zebrafish embryos, CYFIP2 depletion...
The obligate intracellular parasite Toxoplasma gondii causes life-threatening toxoplasmosis to immunocompromised individuals. pathogenesis of relies on its swift dissemination the central nervous system through a 'Trojan Horse' mechanism using infected leukocytes as carriers. Previous work found TgWIP, protein secreted from Toxoplasma, played role in altering actin cytoskeleton and promoting cell migration dendritic cells (DCs). However, behind these changes was unknown. Here, we report that...
Abstract Rho-family GTPase Rac1 activates the WAVE regulatory complex (WRC) to drive Arp2/3-mediated actin polymerization in many essential processes. binds WRC at two distinct sites—the A and D sites. Precisely how binding triggers activation remain unknown. Here we report structures by itself, when bound single or double molecules, ∼3 Å resolutions cryogenic-electron microscopy. The reveal that sites mechanisms, site, but not drives activation. Activation involves a series of unique...
Autolysis is the most important restrictive factor for live sea cucumber trade and commercial transportation. Thus, it essential to investigate mechanism of autolysis activation or deactivation in cucumber. In this study, monodansylcadaverine staining Western blotting experiment methods indicated implication autophagy ultraviolet (UV) exposed cucumbers. The health condition was observed after cucumbers (Stichopus japonicus) were gastric perfusion with autophagic inhibitor (3-methyladenine)...
The obligate intracellular parasite
Abstract Immunodeficiency often coincides with immune hyperresponsiveness such as autoimmunity, lymphoproliferation, or atopy, but the molecular basis of this paradox is typically unknown. We describe four families immunodeficiency coupled cytokine overproduction, hemophagocytic lymphohistocytosis, and autoimmunity. discovered loss-of-function variants in gene NCKAP1L , encoding hematopoietic-specific Hem1 protein. Three mutations cause protein WAVE regulatory complex (WRC) loss, thereby...
This study was designed to explore the relationship between deterioration of sea cucumbers and intestinal flora's diversity change during preservation using 16S rRNA gene sequencing. We show that body wall cucumber develops white spots on day 4, epidermis dissolves 7. The distinctive OTUs remain relatively low four days storage but increase potential gut microbiota biomarkers are Psychrobacter-cibarius, Ralstonia-pickettii, Clostridiales cucumber. result mixed fresh deteriorated validates...
Summary Crosstalk between Rho- and Arf-family GTPases plays an important role in linking actin cytoskeletal remodeling to membrane protrusion, organelle structure, vesicle trafficking. The central regulator, WAVE Regulatory Complex (WRC), is a converging point of Rac1 (a Rho-family GTPase) Arf signaling many processes, but how promotes WRC activation unknown. Here we reconstituted direct interaction WRC. This can be greatly enhanced by binding the D site Arf1 binds newly identified conserved...