The physicochemical characteristics and functional properties of pumpkin (Cucurbita maxima D. var. Cabello de Ángel) pectin obtained by cavitation facilitated extraction from pulp have been evaluated compared with commercial citrus apple pectins. C. had an Mw value 90 kDa a high degree (72%) esterification. cytoprotective antioxidant effects citrus, samples different concentrations were studied in vitro cell lines HT-29 (human colon adenocarcinoma) MDCK1 (canine kidney epithelium). All...

Antioxidant capacity (AOC) against peroxyl radical and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) cation was measured for a series of p-hydroxybenzoic (HB) p-hydroxycinnamic (HC) acids at different pH. Quantum-chemical computation performed using Gaussian 3.0 software package to calculate the geometry energy parameters same compounds. Significant correlations were revealed between AOC number calculated parameters. The most significant descriptors studied...

Laccases are copper-containing oxidases that catalyze a one-electron abstraction from various phenolic and non-phenolic compounds with concomitant reduction of molecular oxygen to water. It is well-known laccases sources have different substrate specificities, but it not completely clear what exactly provides these differences. The purpose this work was study the features specificity four basidiomycete fungi Trametes hirsuta, Coriolopsis caperata, Antrodiella faginea, Steccherinum...

Bioactive peptides derived from milk proteins are an active research area. Exhibiting numerous positive physiological effects on digestive, cardiovascular, immune and nervous systems, these thought to be one of the most promising ingredients for functional food. Generally, inactive within parent can liberated during fermentation by specific proteolytic systems various Lactobacillus spp. Here we present study helveticus NK1, rhamnosus F reuteri LR1 strains. It was demonstrated that...

Recent consumer demand for non-dairy alternatives has forced many manufacturers to turn their attention cereal-based non-alcoholic fermented products. In contrast dairy products, there is no defined and standardized starter culture manufacturing Since spontaneous fermentation rarely suitable large-scale commercial production, it not surprising that have started adopt centuries-known starters based on lactic acid bacteria (LABs) the of cereals. However, little known about processes cereals...

This paper describes the structural analysis of native form laccase from Trametes hirsuta at 1.8 Å resolution. structure provides a basis for elucidation mechanism catalytic action these ubiquitous proteins. The resolution provided good level detail compared with many previously reported structures. A brief comparison active sites other laccases is given.

AbstractSome RNases selectively attack malignant cells, triggering an apoptotic response, and therefore are considered as alternative chemotherapeutic drugs. Here we studied the effects of Bacillus intermedius RNase (binase) on murine myeloid progenitor cells FDC-P1; transduced FDC-P1 ectopically expressing mutated human KIT N822K oncogene and/or AML1-ETO oncogene; leukemia Kasumi-1 both these oncogenes. Expression oncogenes makes sensitive to toxic binase. were most responsive actions...

Quantum chemical methods allow screening and prediction of peptide antioxidant activity on the basis known experimental data. It can be used to design selective proteolysis protein sources in order obtain products with activity. Molecular geometry electronic descriptors redox-active amino acids, as well tyrosine methionine-containing dipeptides, were studied by Density Functional Theory method. The calculated data was reveal several responsible for capacities model compounds based their...

Ligninolytic heme peroxidases comprise an extensive family of enzymes, which production is characteristic for white-rot Basidiomycota. The majority fungal are encoded by multigene families that differentially express closely related proteins. Currently, there were very few attempts to characterize the complete in a single fungus. Here we focusing on identification and characterization peroxidase genes, transcribed secreted basidiomycete Trametes hirsuta 072, efficient lignin degrader. T....

Lacticaseibacillus paracasei (formerly Lactobacillus paracasei) is a nomadic lactic acid bacterium (LAB) that inhabits wide variety of ecological niches, from fermented foodstuffs to host-associated microenvironments. Many the isolated L. strains have been used as single-strain probiotics or part symbiotic consortium within formulations. The present study contributes exploration different derived non-conventional isolation sources-the South African traditional drink mahewu (strains MA2 and...

The laccase from Steccherinum murashkinskyi is a member of the large family multicopper oxidases that catalyze oxidation wide range organic and inorganic substrates, accompanied by reduction dioxygen to water. reducing properties X-ray radiation high quality crystals allow study catalytic water directly in crystal. A series diffraction data sets with increasing absorbed dose were collected single crystal at 1.35 Å resolution. Changes active-site structure associated molecular oxygen on...

Laccase is one of the oldest known and intensively studied fungal enzymes capable oxidizing recalcitrant lignin-resembling phenolic compounds. It currently well established that genomes almost always contain several non-allelic copies laccase genes (laccase multigene families); nevertheless, many aspects multigenicity, for example, their precise biological functions or evolutionary relationships, are mostly unknown. Here, we present a detailed analysis sensu stricto (CAZy - AA1_1) from fungi...

Systematical consumption of functional products has a significant positive effect on health and can reduce the risk diseases. The aim this study was to investigate possibility using whey protein hydrolysate (WPH) pumpkin pectin as ingredients in mousse, evaluate mousse’s antioxidant hypotensive activities vitro, long-term intake mousse samples progression hypertension spontaneously hypertensive rats (SHRs) microbiome status Wistar with antibiotic-induced dysbiosis. experimental vitro...

In this study, four commercialized indigenous fermented beverages most highly consumed in Russia (kefir and ryazhenka) South Africa (amasi mahewu) were analyzed for their potential health-promoting properties flavor-forming volatile organic compounds (VOC). The analysis of antioxidant capacity demonstrated superiority dairy-based (kefir, ryazhenka amasi) over the corn-based mahewu; however, mahewu outperformed terms its antihypertensive effect (i.e., ability to inhibit angiotensin I...

White-rot basidiomycete fungi are a unique group of organisms that evolved an unprecedented arsenal extracellular enzymes for efficient degradation all components wood such as cellulose, hemicelluloses and lignin. The exoproteomes white-rot represent natural enzymatic toolbox white biotechnology. Currently, only narrow taxonomic fungi—fungi belonging to the Polyporales order—are extensively studied. In this article, two fungi, Peniophora lycii LE-BIN 2142 from Russulales order Trametes...

White-rot basidiomycetes from the poorly studied residual polyporoid clade of Polyporales order Junghuhnia nitida (Pers.) Ryvarden and Steccherinum bourdotii Saliba & A. David grow as secondary xylotrohps on well decomposed woody materials. The main objective current study was to compare oxidative potential, growth, production enzymes laccase properties J. S. with that typical primary Trametes hirsuta (Wulfen) Lloyd Coriolopsis caperata (Berk.) Murrill, belonging core clade. For first time...