Laccases are copper-containing oxidases that catalyze a one-electron abstraction from various phenolic and non-phenolic compounds with concomitant reduction of molecular oxygen to water. It is well-known laccases sources have different substrate specificities, but it not completely clear what exactly provides these differences. The purpose this work was study the features specificity four basidiomycete fungi Trametes hirsuta, Coriolopsis caperata, Antrodiella faginea, Steccherinum...

Bioactive peptides derived from milk proteins are an active research area. Exhibiting numerous positive physiological effects on digestive, cardiovascular, immune and nervous systems, these thought to be one of the most promising ingredients for functional food. Generally, inactive within parent can liberated during fermentation by specific proteolytic systems various Lactobacillus spp. Here we present study helveticus NK1, rhamnosus F reuteri LR1 strains. It was demonstrated that...

Ligninolytic heme peroxidases comprise an extensive family of enzymes, which production is characteristic for white-rot Basidiomycota. The majority fungal are encoded by multigene families that differentially express closely related proteins. Currently, there were very few attempts to characterize the complete in a single fungus. Here we focusing on identification and characterization peroxidase genes, transcribed secreted basidiomycete Trametes hirsuta 072, efficient lignin degrader. T....

Lacticaseibacillus paracasei (formerly Lactobacillus paracasei) is a nomadic lactic acid bacterium (LAB) that inhabits wide variety of ecological niches, from fermented foodstuffs to host-associated microenvironments. Many the isolated L. strains have been used as single-strain probiotics or part symbiotic consortium within formulations. The present study contributes exploration different derived non-conventional isolation sources-the South African traditional drink mahewu (strains MA2 and...

Recent consumer demand for non-dairy alternatives has forced many manufacturers to turn their attention cereal-based non-alcoholic fermented products. In contrast dairy products, there is no defined and standardized starter culture manufacturing Since spontaneous fermentation rarely suitable large-scale commercial production, it not surprising that have started adopt centuries-known starters based on lactic acid bacteria (LABs) the of cereals. However, little known about processes cereals...

Laccase is one of the oldest known and intensively studied fungal enzymes capable oxidizing recalcitrant lignin-resembling phenolic compounds. It currently well established that genomes almost always contain several non-allelic copies laccase genes (laccase multigene families); nevertheless, many aspects multigenicity, for example, their precise biological functions or evolutionary relationships, are mostly unknown. Here, we present a detailed analysis sensu stricto (CAZy - AA1_1) from fungi...

In this study, four commercialized indigenous fermented beverages most highly consumed in Russia (kefir and ryazhenka) South Africa (amasi mahewu) were analyzed for their potential health-promoting properties flavor-forming volatile organic compounds (VOC). The analysis of antioxidant capacity demonstrated superiority dairy-based (kefir, ryazhenka amasi) over the corn-based mahewu; however, mahewu outperformed terms its antihypertensive effect (i.e., ability to inhibit angiotensin I...

White-rot basidiomycete fungi are a unique group of organisms that evolved an unprecedented arsenal extracellular enzymes for efficient degradation all components wood such as cellulose, hemicelluloses and lignin. The exoproteomes white-rot represent natural enzymatic toolbox white biotechnology. Currently, only narrow taxonomic fungi—fungi belonging to the Polyporales order—are extensively studied. In this article, two fungi, Peniophora lycii LE-BIN 2142 from Russulales order Trametes...

White-rot basidiomycetes from the poorly studied residual polyporoid clade of Polyporales order Junghuhnia nitida (Pers.) Ryvarden and Steccherinum bourdotii Saliba & A. David grow as secondary xylotrohps on well decomposed woody materials. The main objective current study was to compare oxidative potential, growth, production enzymes laccase properties J. S. with that typical primary Trametes hirsuta (Wulfen) Lloyd Coriolopsis caperata (Berk.) Murrill, belonging core clade. For first time...

Steccherinum ochraceum is a white rot basidiomycete with wide ecological amplitude. It occurs in different regions of Russia and throughout the world, occupying climatic zones. S. colonizes stumps, trunks, branches various deciduous (seldom coniferous) trees. As secondary colonizing fungus, mainly observed at late decay stages. Here, we present de novo assembly annotation genome ochraceum, LE-BIN 3174. This 8th published fungus from residual polyporoid clade first Steccherinaceae family. The...

The expansion of multiple drug resistant (MDR) strains Klebsiella pneumoniae presents an immense threat for public health. Annually, this microorganism causes thousands lethal nosocomial infections worldwide. Currently, it has been shown that certain lactic acid bacteria (LAB) can efficiently inhibit growth K. and the formation its biofilms; however, active principle such action remains unknown. In current article, inhibition MDR by two LAB-Limosilactobacillus reuteri LR1 Lacticaseibacillus...

Currently, functional dairy products pave a promising way for the prophylaxis of essential hypertension, and search new strains capable producing such is constant challenge scientists around world. In this study, antihypertensive properties milk fermented with several traditional yogurt starters (Lactobacillus delbrueckii Lb100 Lb200; Lactococcus lactis dlA, AM1 MA1; Streptococcus thermophilus 159 16t) one strain non-conventional probiotic starter (Lacticaseibacillus paracasei ABK) were...

A standard draft genome sequence of the white rot saprotrophic fungus Trametes hirsuta 072 (Basidiomycota, Polyporales) is presented. The contains about 33.6 Mb assembled in 141 scaffolds with a G+C content ~57.6%. annotation predicts 14,598 putative protein-coding open reading frames (ORFs).

Here we present the results of exploration laccase multigene families (MGFs) in basidiomycetous fungi from different taxonomic groups using a next generation sequencing (NGS) technology. In our study, multiple genes were identified all investigated (13 species) Polyporaceae, Phanerochaetaceae, Meruliaceae, Pleurotaceae, Physalacriaceae, and Peniophoraceae families. It was shown that phylogenetic positioning newly sequences exhibit patterns clusterization with respect to enzyme properties....

Although, currently, more than 100 laccases have been purified from basidiomycete fungi, the majority of these were obtained fungi Polyporales order, and only scarce data are available about other fungi. In this article, laccase production by white-rot fungus Peniophora lycii, belonging to Russulales was investigated. It shown that, under copper induction, secreted three different isozymes. Two isozymes—Lac5 LacA—were their corresponding nucleotide sequences determined. Both relatively...

Laccases are blue multi-copper oxidases with an extensive number of actual and potential industrial applications. It is known that laccases from different fungal strains may vary in properties; however, the reason this remains unclear. In current study we have isolated characterized seven Steccherinum ochraceum obtained regions central Russia. Although all had same primary sequences, there was a little variation their molecular weights thermostabilities. Moreover, statistically significant...

Incorporation of probiotic Lacticaseibacillus paracasei into a standard yogurt starter culture can drastically improve its health promoting properties. However, besides being an advantage in itself, the incorporation new strain significantly affect overall composition fermented milk. In this article, effect L. strains (KF1 and MA3) several cultures (consisting following strains: Streptococcus thermophilus 16t either Lactobacillus delbrueckii Lb100 or Lb200) was investigated. Such parameters...