A5049621302- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Fungal and yeast genetics research
- Microbial Metabolism and Applications
- Enzyme-mediated dye degradation
- Fungal Biology and Applications
- Studies on Chitinases and Chitosanases
- Microbial Natural Products and Biosynthesis
- Mycotoxins in Agriculture and Food
- CRISPR and Genetic Engineering
- Plant Pathogens and Fungal Diseases
- Microbial Metabolites in Food Biotechnology
- Plant-Microbe Interactions and Immunity
- Enzyme Catalysis and Immobilization
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Microbial Metabolic Engineering and Bioproduction
- Endoplasmic Reticulum Stress and Disease
- Polysaccharides and Plant Cell Walls
- Nuclear Structure and Function
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- Biochemical and biochemical processes
- Steroid Chemistry and Biochemistry
- melanin and skin pigmentation
Russian Academy of Sciences
2009-2024
Federal Center Research Fundamentals of Biotechnology
2016-2024
A N Bach Institute of Biochemistry
2009-2023
Biotechnology Research Center
2023
Genetika
2002-2008
Laccase is one of the oldest known and intensively studied fungal enzymes capable oxidizing recalcitrant lignin-resembling phenolic compounds. It currently well established that genomes almost always contain several non-allelic copies laccase genes (laccase multigene families); nevertheless, many aspects multigenicity, for example, their precise biological functions or evolutionary relationships, are mostly unknown. Here, we present a detailed analysis sensu stricto (CAZy - AA1_1) from fungi...
Recently, the study of chitinases has become an important target numerous research projects due to their potential for applications, such as biocontrol pest agents. Plant from carnivorous plants genus Drosera are most aggressive against a wide range phytopathogens. However, low solubility or insolubility protein hampered application biofungicides. To obtain plant chitinase in soluble form E.coli expression strains, three different approaches including dialysis, rapid dilution, and refolding...
Мицелиальный гриб Penicillium verruculosum (ВКМ F-3972D) является высокопродуктивным продуцентом комплекса ферментов, участвующих в биодеградации полимеров растительной клеточной стенки. Секреторная способность P. достигает 60 г/л внеклеточного белка, что позволяет использовать его качестве базового продуцента ферментов и ферментных комплексов, востребованных промышленными биотехнологиями. На базе гриба P.verruculosum была разработана система экспрессии, основанная на использовании сильного...
Мицелиальный гриб P. verruculosum является сапрофитным грибом-целлюлолитиком. Ранее с помощью многостадийного неупорядоченного мутагенеза был получен промышленный штамм-продуцент целлюлазных препаратов B1-221-151, обладающий продуктивностью до 60 г секретируемого белка на литр культуральной жидкости. Основными ферментами его секретирумого комплекса являются целлобиогидролаза 1 и 2 (до 50%), эндоглюканазы 12%), β-глюкозидаза 4%), ксиланазы. Также ранее из штамма рецепиентный штамм В1-537...
A new nag1 gene encoding N-acetyl-D-glucosaminidase from Streptomyces kursanovii was cloned. The soluble form of obtained after improving its expression in E. coli ArcticExpressTM (DE3) cells. homogeneous the enzyme isolated by affinity chromatography, and bacteriolytic ability against cells Gram-positive bacterium Micrococcus lysodeikticus studied. It demonstrated that enzyme's capacity surpasses chicken lysozyme.
The filamentous fungus Penicillium verruculosum (anamorph Talaromyces verruculosus) has been shown to be an efficient producer of secreted cellulases, used in biorefinery processes. Understanding the mechanisms regulation cellulase gene expression P. is a current task industrial biotechnology, since it allows for targeted changes composition complex by fungus. Expression genes fungi regulated mainly at level transcription via pathway-specific factors (TF), majority which belong Zn(II)2Cys6...