A5038214218- Antibiotic Resistance in Bacteria
- Respiratory Support and Mechanisms
- Cardiac, Anesthesia and Surgical Outcomes
- Hemodynamic Monitoring and Therapy
- Pulmonary Hypertension Research and Treatments
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Central Venous Catheters and Hemodialysis
- Inhalation and Respiratory Drug Delivery
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Vascular Procedures and Complications
- Anesthesia and Pain Management
- Venous Thromboembolism Diagnosis and Management
- Airway Management and Intubation Techniques
- Bacterial Infections and Vaccines
- Mechanical Circulatory Support Devices
- Immune Response and Inflammation
- Antibiotic Use and Resistance
- Phosphorus and nutrient management
- Clostridium difficile and Clostridium perfringens research
- Anesthesia and Sedative Agents
- Bacteriophages and microbial interactions
- Non-Invasive Vital Sign Monitoring
- Streptococcal Infections and Treatments
- Adsorption and biosorption for pollutant removal
Kyorin University
2016-2025
Kyorin University Hospital
2025
Weatherford College
2023
Clinical Research Management
2019
Society of Interventional Radiology
2019
Keio University
2004-2010
University of California, San Francisco
2003-2008
Ono Pharmaceutical (Japan)
2006
Kyoto Prefectural University of Medicine
2006
Tokyo Women's Medical University
2006
Proinflammatory cytokines play an important role in ventilator-induced lung injury (VILI). High-mobility group box-1 (HMGB1) is a macrophage-derived proinflammatory cytokine that can cause injury.This study tested the hypothesis HMGB1 released intact lungs ventilated with large Vt. A second objective was to identify source of HMGB1. third examine effects blocking on subsequent development VILI.Bronchoalveolar lavage fluid (BALF) and tissues were obtained from rabbits mechanically for 4 h...
We have implemented several preventive measures to reduce central line-associated bloodstream infection (CLABSI) in the general intensive care unit (ICU) of a university hospital Japan. Here, we analyzed factors associated with CLABSI patients venous catheter (CVC) insertions and evaluated effects our measures. From July 2013 June 2018, data was collected from medical records 1472 1635 CVC insertions, including age, sex, Acute Physiology Chronic Health Evaluation II (APACHE II) score,...
The relative contributions of the cytotoxic phenotype P. aeruginosa expressing type III secretory toxins and an immunocompromised condition lacking normal Toll-like receptor 4 (TLR4) signaling in pathogenesis acute lung injury sepsis were evaluated a mouse model for Pseudomonas pneumonia. By using lipopolysaccharide-resistant C3H/HeJ mice missing TLR4 due to mutation on tlr4 gene, we how modulates pneumonia caused by toxins.We infected or C3H/FeJ with three different doses either strain...
Objective: Acute lung injury in Pseudomonas aeruginosa pneumonia depends primarily on ExoU toxin being delivered directly into the eukaryotic cell cytosol through type III secretion system. The amino-acid sequence of has a potato patatin-like phospholipase domain, similar to mammalian Ca2+-independent A2. We examined whether acute caused by cytotoxic P. was dependent domain ExoU. Design: Laboratory investigation using an established mouse model for with quantitative measurements and...
Background In the partial CO(2) rebreathing method, monitored changes in elimination and end-tidal response to a brief period are used estimate cardiac output. However, dynamic production during ischemia reperfusion may affect accuracy of these estimates. This study was designed compare measurements output as produced by (NICO), bolus (BCO), continuous thermodilution (CCO) methods monitoring Methods Cardiac continuously using both NICO CCO 28 patients undergoing aortic reconstruction. BCO...
Purpose Chronic pain after cesarean section (CS) is a serious concern, as it can result in functional disability. We evaluated the prevalence of chronic CS prospectively at single institution Japan. also analyzed perioperative risk factors associated with using logistic regression analyses backward-stepwise procedure. Materials and Methods Patients who underwent elective or emergency between May 2012 2014 were recruited. Maternal demographics well details surgery anesthesia recorded. An...
Ventilation with a small tidal volume (V(t)) is associated better clinical outcomes than large V(t), particularly in critical settings, including acute lung injury. To determine whether V(t) influences the lipopolysaccaharide (LPS) recognition pathway, we studied CD14 expression rabbit lungs and release of TNF-alpha by cultured alveolar macrophages after 240 min ventilation (20 ml/kg) vs. (5 V(t). We also applied or to instilled 50 microg/kg LPS. The collected revealed 20-fold increase...
ABSTRACT Virulent P. aeruginosa strains express PcrV, one of the translocational components type III secretion system. PcrV has been reported to be a protective antigen against lethal infection. The region, which contributes immunity infection, was investigated by using genetically engineered, truncated proteins and affinity‐purified anti‐PcrV antibodies proteins. efficacy active passive immunization tested in mice with cyclophosphamide‐induced immunosuppression intraabdominal challenge ....
An effective vaccine against Pseudomonas aeruginosa would be hugely beneficial to people who are susceptible the serious infections it can cause. Vaccination PcrV of P. type III secretion system is a potential prophylactic strategy for improving incidence and prognosis pneumonia. Here, effect nasal adjuvanted with CpG oligodeoxynucleotide (CpG) was compared PcrV/aluminum hydroxide gel (alum) vaccine. Seven groups mice were vaccinated intranasally one following: 1, PcrV-CpG; 2, PcrV-alum; 3,...
ABSTRACT Vaccination against the type III secretion system of P. aeruginosa is a potential prophylactic strategy for reducing incidence and improving poor prognosis pneumonia. In this study, efficacies three different adjuvants, Freund's adjuvant (FA), aluminum hydroxide (alum) CpG oligodeoxynucleotide (ODN), were examined from viewpoint inducing PcrV‐specific immunity virulent . Mice that had been immunized intraperitoneally with recombinant PcrV formulated one above adjuvants challenged...
Objective Acute lung injury induced by overstretch is associated with neutrophil influx, but the pathogenic role of neutrophils in overstretch-induced remains unclear. Design To assess contribution neutrophils, we compared effects noninjurious large tidal volume (Vt) ventilation on lungs normal and neutrophil-depleted animals. Setting Research animal laboratory. Subjects Twenty-six male Japanese white rabbits. Interventions Animals were mechanically ventilated for 4 hrs one three following...
Purpose Pulse oximetry is routinely used to continuously and non-invasively monitor arterial oxygen saturation (SaO2). When by pulse oximeter (SpO2) overestimates SaO2, hypoxemia may be overlooked. We compared the SpO2 - SaO2 differences among three oximeters in patients with chronic thromboembolic pulmonary hypertension (CTEPH) who spent their daily lives a poor state. Material Method This prospective observational study recruited 32 CTEPH undergoing elective cardiac catheterization. As we...
The PcrV cap structure of the type III secretory apparatus Pseudomonas aeruginosa is a vaccine target. Human immunoglobulin G (IgG) molecules extracted from sera containing high or low anti-PcrV titers were tested for their effects against P. pneumonia in mouse model. Among 198 volunteers, we selected top 10 titer and bottom IgG fraction each serum sample. First, examined virulent aeruginosa. A lethal dose premixed with saline, human IgG, rabbit-derived polyclonal was intratracheally...