A5022893522- Circadian rhythm and melatonin
- Regulation of Appetite and Obesity
- Biochemical Analysis and Sensing Techniques
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- Photoreceptor and optogenetics research
- Adipose Tissue and Metabolism
- Physiological and biochemical adaptations
- Cell death mechanisms and regulation
- Stress Responses and Cortisol
- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Growth Hormone and Insulin-like Growth Factors
- Aquaculture Nutrition and Growth
- Mass Spectrometry Techniques and Applications
- Neuropeptides and Animal Physiology
- Sleep and Wakefulness Research
- Birth, Development, and Health
- Hypothalamic control of reproductive hormones
- Receptor Mechanisms and Signaling
- Kruppel-like factors research
- Genetics, Bioinformatics, and Biomedical Research
- Hedgehog Signaling Pathway Studies
- Medicinal Plants and Bioactive Compounds
- Zebrafish Biomedical Research Applications
Universidad Complutense de Madrid
2000-2023
Centro de Investigaciones Biológicas Margarita Salas
2002-2009
Consejo Superior de Investigaciones Científicas
2002-2007
Anna Needs Neuroblastoma Answers
2005
Uppsala University
2002-2003
Emory University
1999-2000
Abstract Programmed cell death is an essential, highly regulated process in neural development. Although the role of insulin‐like growth factor I supporting survival cells has been well characterized, studies on proinsulin/insulin are scarce. Here, we characterize effects embryonic day 15.5 mouse retina. Both proinsulin mRNA and immunoreactivity were found developing Organotypic retinas cultured under deprivation showed increase that was reversed by proinsulin, insulin I, with similar median...
Ghrelin is a gut-brain peptide hormone, which binds to the growth hormone secretagogue receptor (GHS-R) regulate wide variety of biological processes in fish. Despite these prominent physiological roles, no studies have reported anatomical distribution preproghrelin transcripts using situ hybridization non-mammalian vertebrate, and its mapping within different encephalic areas remains unknown. Similarly, information available on possible 24-h variations expression any vertebrate species. The...
Abstract Glucose homeostasis is an important biological process that involves a variety of regulatory mechanisms. This study aimed to determine whether ghrelin, multifunctional gut-brain hormone, modulates intestinal glucose transport in goldfish ( Carassius auratus ). Three transporters, the facilitative transporter 2 (GLUT2), and sodium/glucose co-transporters 1 (SGLT1) (SGLT2), were studied. Immunostaining sections found colocalization ghrelin GLUT2 SGLT2 mucosal cells. Some cells...
The anoretic effect of corticotropin-releasing factor (CRF) was not dependent on adrenal activation in goldfish (Carassius auratus). Moreover, an interaction between CRF and the hypothalamic catecholaminergic system central regulation food intake observed. intracerebroventricular (icv) administration increased cortisol levels reduced norepinephrine dopamine content at 2 hr postinjection, with these effects reversed by alpha-helical CRF[9-41] pretreatment. independent circulating increase,...
Abstract Programmed cell death occurs during both early and late neural development. The mechanisms for the regulation execution of as well its developmental role are still not fully understood. In this work we have studied programmed in retinal neuroepithelium. Apoptotic cells were selectively located around optic nerve head neuroepithelium 2‐ to 6‐day‐old chick embryos. TUNEL‐positive which immunostained activated caspase‐3 showed overlapping distributions suggesting that is involved...
Abstract Programmed cell death is a genuine developmental process of the nervous system, affecting not only projecting neurons but also proliferative neuroepithelial cells and young neuroblasts. The embryonic chick retina has been employed to correlate in vivo vitro studies on regulation. We characterize here role two major signaling pathways, PI3K‐Akt MEK‐ERK, controlled retinal organotypic cultures from day 5 (E5) E9, when preferentially affects proliferating ganglion neurons,...
Abstract Neuronal cell death is a genuine developmental process, with precise regulation and defined roles. In striking contrast, characterization of that occurs at early stages neural development very limited. We previously showed embryonic proinsulin increases the level chaperone heat shock cognate 70 (Hsc70) reduces incidence apoptosis in neurulating chick embryo [de la Rosa, et al. (1998), Proc. Natl. Acad. Sci. USA , 95 9950]. now demonstrate Hsc70 directly involved survival during...
Tyrosine hydroxylase (TH) is the first and rate-limiting enzyme in catecholamine biosynthesis. Whereas neuroendocrine roles of cathecolamines postnatally are well known, presence function TH organogenesis unclear. The aim this study was to define expression during cardiac development unravel role it may play heart formation. We studied chick embryos by whole mount situ hybridization quantitative reverse transcription-polymerase chain reaction analysed activity high-performance liquid...
Abstract Transforming growth factor (TGF)‐β and insulin display opposite effects in regulating programmed cell death during vertebrate retina development; the former induces apoptosis while latter prevents it. In present study we investigated coordinated actions of TGF‐β an organotypic culture system early postnatal mouse retina. Addition exogenous resulted a significant increase whereas attenuated was capable blocking TGF‐β‐induced apoptosis. This effect appeared to be modulated via...
Ghrelin is the only known hormone posttranslationally modified with an acylation. This modification crucial for most of ghrelin’s physiological effects and catalyzed by polytopic enzyme ghrelin O-acyltransferase (GOAT). The aim this study was to characterize GOAT in a teleost model, goldfish (Carassius auratus). First, full-length cDNA sequence obtained RT-PCR rapid amplification ends methods. Two highly homologous cDNAs 1491 1413 bp, respectively, named goat-V1 goat-V2 were identified....
Ghrelin O-acyltransferase (GOAT) is the enzyme responsible for acylation of ghrelin, a gut-brain hormone with important roles in many physiological functions vertebrates. Many aspects GOAT remain to be elucidated, especially fish, and particularly its anatomical distribution within different brain areas has never been reported date. The present study aimed characterize mapping using RT-qPCR immunohistochemistry teleost, goldfish (Carassius auratus). Results show that goat transcripts are...
Proliferation, cell death and differentiation occur simultaneously in developing retina are precisely orchestrated. We have studied the effects of biotin (vitamin H) on early retinal development. In vivo administration to embryonic chick eyes at moderately elevated levels induced malformations, affecting lens structures. The were strictly age dependent only found embryos treated between Hamburger Hamilton stage 14–17. Biocytin, a analogue, mimicked effects, while avidin could block effects....