A5081820802- Mesenchymal stem cell research
- dental development and anomalies
- Electrospun Nanofibers in Biomedical Applications
- Bone and Dental Protein Studies
- Periodontal Regeneration and Treatments
- Cancer Cells and Metastasis
- Endodontics and Root Canal Treatments
- Bone Tissue Engineering Materials
- Glioma Diagnosis and Treatment
- Spinal Hematomas and Complications
- Angiogenesis and VEGF in Cancer
- Autophagy in Disease and Therapy
- Spine and Intervertebral Disc Pathology
- Bone and Joint Diseases
- Cancer Genomics and Diagnostics
- Chemokine receptors and signaling
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Neonatal Respiratory Health Research
University of Michigan
2010-2024
Kobe University
2021
Angiogenesis Foundation
2010
Studies on mechanisms underlying the differentiation of dental pulp stem cells are critical for understanding biology odontogenesis and tissue engineering. Here, we tested hypothesis that from exfoliated deciduous teeth (SHED) differentiate into functional odontoblasts endothelial cells. SHED were seeded in tooth slice/scaffolds implanted subcutaneously immunodeficient mice. differentiated generated tubular dentin, as determined by tetracycline staining confocal microscopy. These also...
The clinical translation of stem-cell-based dental pulp regeneration will require the use injectable scaffolds. Here, we tested hypothesis that stem cells from exfoliated deciduous teeth (SHED) can generate a functional when injected into full-length root canals. SHED survived and began to express putative markers odontoblastic differentiation after 7 days mixed with Puramatrix™ (peptide hydrogel), or 14 recombinant human Collagen (rhCollagen) type I, canals premolars in vitro. Roots...
It is known that stem cells from exfoliated deciduous teeth (SHED) can be induced to differentiate into odontoblasts. However, the nature of dentin-derived morphogenic signals required for dental pulp cell differentiation remains unclear. The hypothesis underlying this work Bone Morphogenetic Proteins (BMP) are necessary SHED We observed express markers odontoblastic (DSPP, DMP-1, MEPE) when seeded in human tooth slice/scaffolds and cultured vitro, or implanted subcutaneously immunodeficient...
The quest for finding a suitable scaffold system that supports cell survival and function and, ultimately, the regeneration of pulp–dentin complex remains challenging. Herein, we hypothesized dental pulp stem cells (DPSCs) encapsulated in collagen-based hydrogel with varying stiffness would regenerate functional dentin when concentrically injected into tooth slices. Collagen hydrogels concentrations 3 mg/mL (Col3) 10 (Col10) were prepared, their microstructure assessed using rheometer...
The discovery that dental pulp stem cells are capable of differentiating into endothelial raises the exciting possibility these can be a single source odontoblasts and vascular networks in tissue engineering. purpose this study was to begin define signaling pathways regulate differentiation SHED. Stem from exfoliated deciduous teeth (SHED) exposed growth medium (EGM-2MV) supplemented with factor (VEGF) differentiated VEGFR2-positive CD31-positive vitro. In vivo, VEGFR1-silenced SHED seeded...
In previous studies, fluorapatite (FA) crystal-coated surfaces have been shown to stimulate the differentiation and mineralization of human dental pulp stem cells (DPSCs) in two-dimensional cell culture. However, whether FA surface can recapitulate these properties three-dimensional culture is still unknown. This study examined differences behavior DPSCs cultured on electrospun polycaprolactone (PCL) NanoECM nanofibers with or without crystals. Under near-physiologic conditions, crystals...
Interactions with the microenvironment modulate fate of stem cells in perivascular niches tissues (e.g., bone) and organs liver). However, functional relevance molecular crosstalk between endothelial within niche dental pulps is unclear. Here, we tested hypothesis that cell–initiated signaling necessary to maintain self-renewal pulp cells. Confocal microscopy showed ALDH1 high Bmi-1 are preferentially localized close proximity blood vessels physiological human pulps. Secondary orosphere...
In the largest avascular low-nutrient intervertebral disc, resident cells would utilize autophagy, a stress-response survival mechanism by self-digestion and recycling wastes. Our goal was to elucidate involvement of autophagy in disc homeostasis through RNA interference autophagy-related gene 5 (Atg5).In vitro, small interfering RNAs (siRNAs) targeting autophagy-essential Atg5 were transfected into rat cells. Cell viability with levels including expression, apoptosis, senescence assessed...
As a major intracellular degradation and recycling machinery, autophagy plays an important role in maintaining cellular homeostasis remodeling during normal development. Our previous study showed that fluorapatite (FA) crystal-coated electrospun polycaprolactone (PCL) was capable of inducing differentiation mineralization human dental pulp stem cells. However, how changes whether vital these processes is still unknown. In this study, we seeded STEMPRO adipose-derived cells (ASCs) on both...
<h3>BACKGROUND AND PURPOSE:</h3> Increased angiogenesis in head and neck squamous cell carcinoma correlates to more aggressive tumors with increased morbidity. Because both elevated blood flow high serum CXCL8 levels are correlated angiogenesis, our objective was see if measured CT perfusion levels, thereby helping identify candidates for targeted therapies that inhibit the Bcl-2 proangiogenic pathway associated CXCL8. <h3>MATERIALS METHODS:</h3> Seven patients locally recurrent or...
Supplemental material, DS_10.1177_0022034520925417 for Endothelial-Initiated Crosstalk Regulates Dental Pulp Stem Cell Self-Renewal by M. Oh, Z. Zhang, A. Mantesso, A.E. Oklejas and J.E. Nor in Journal of Research