A5069780757- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Environmental Impact and Sustainability
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Land Use and Ecosystem Services
- Epigenetics and DNA Methylation
- Gene expression and cancer classification
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- RNA Research and Splicing
- Energy, Environment, Economic Growth
- Genomic variations and chromosomal abnormalities
- Natural product bioactivities and synthesis
- Plant Disease Resistance and Genetics
- Urban Green Space and Health
- Soybean genetics and cultivation
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Multi-Criteria Decision Making
- Computational Drug Discovery Methods
- Protein Kinase Regulation and GTPase Signaling
- RNA modifications and cancer
- MicroRNA in disease regulation
Temasek Life Sciences Laboratory
2023-2025
National University of Singapore
2023-2025
Yunnan University
2020-2024
Center for Life Sciences
2020-2024
Minzu University of China
2024
Southwest University of Science and Technology
2007-2024
Institute of Zoology
2024
Guangdong Academy of Sciences
2024
Guangzhou Quality Supervision, Inspection and Research Institute
2024
Tsinghua University
2024
A current question in the high-order organization of chromatin is whether topologically associating domains (TADs) are distinct from other hierarchical domains. However, due to unclear TAD definition tradition, structural and functional uniqueness not well studied. In this work, we refined by further constraining TADs optimal separation on global intra-chromosomal interactions. Inspired constraint, developed a novel method, called HiTAD, detect Hi-C HiTAD performs domain sensitivity,...
The 3D chromatin structure modeling by interactions derived from Hi-C experiments is significantly challenged the intrinsic sequencing biases in these experiments. Conventional methods only focus on bias among different regions within same experiment but neglect arising experimental depth. We now show that regional interaction tightly coupled with depth, and we further identify a parameter as inherent characteristics of data for regions. Then present an approach prediction capable relaxing...
Summary Floral organ identity in plants is controlled by floral homeotic A/B/C/D/E‐class genes. In Arabidopsis thaliana, several epigenetic repressors that regulate these genes have been characterized. However, the roles of factors rice development not explored detail. Here, we report identification and functional characterization a repressor, DEFORMED FLORAL ORGAN 1 ( DFO ) gene, which causes abnormal morphology when mutated. We isolated dfo1 mapping, confirmed its function rescue...
Marker segregation distortion is a natural phenomenon. Severely distorted markers are usually excluded in the construction of linkage maps. We investigated effect marker on map and quantitative trait locus (QTL) mapping. A total 519 recombinant inbred lines soybean from orthogonal reciprocal crosses between LSZZH NN493-1 were genotyped by specific length amplified fragment seed linoleic acid content was measured three environments. As result, twenty groups constructed with 11,846 markers,...
The intensification of land use and the accelerated integration three industries (agricultural production, agricultural product processing industry, market service industry) in China have resulted continuous expansion animal husbandry its industrial chain. This phenomenon has led to a rise greenhouse gas (GHG) emissions from livestock farming, intensifying climate change placing strain on worldwide environmental conservation efforts. Life cycle assessment (LCA) was utilized evaluate carbon...
Urban green space can bring various ecosystem benefits to diverse social groups. Among those benefits, intangible values are often neglected but highly relevant human welfare. Existing research on the of urban focusses perspective inhabitants rather than tourists, even though tourists also major beneficiaries. By combining different data sources into a comprehensive source about green-space values, we investigated disparity between inhabitants' and tourists' perceptions space-associated...
Focal adhesion kinase (FAK) is one kind of tyrosine kinases that modulates integrin and growth factor signaling pathways, which a promising therapeutic target because involving in cancer cell migration, proliferation, survival. To investigate the mechanism between FAK triazinic inhibitors design high activity inhibitors, molecular modeling integrated with 3D-QSAR, docking, dynamics simulations, binding free energy calculations was performed. The optimum CoMFA CoMSIA models showed good...
In order to rationalize material selection in green design, this study presents an attempt combine the methods of generalized Data Envelopment Analysis (DEA) and Technique for Order Preference by Similarity Ideal Solution (TOPSIS). By establishing a index system, G-CCR model DEA was first used select effective materials from candidate samples, TOPSIS then sort suppliers. The combined DEA/TOPSIS helps rank quality, integrate both merits ofG-CCR key characteristics TOPSIS. results showed that...
Abstract Simple sequence repeats (SSRs) derived from expressed tags (ESTs) are valuable markers because they represent transcribed regions and often have putative functions. We mined characterized microsatellites in melon ESTs. Three hundred eighty‐three SSR loci were identified 309 of 3188 unigenes assembled by 5747 EST mRNA sequences GenBank with occurring frequency 1/4.7 kb. Twenty‐two polymorphic EST‐SSR developed the mean allele number 2.9 per locus expected heterozygosity 0.442....
Recent chromosome conformation capture (3C) derived techniques have revealed that topologically associating domain (TAD) is a pervasive element in chromatin three-dimensional (3D) organization. However, there currently no parameter to quantitatively measure the structural characteristics of TADs, thus obscuring our understanding on and functional differences among TADs. Based finding exist intrinsic interaction patterns we define theoretical parameter, called aggregation preference (AP),...
Abstract The recent discovery of inhibitory compounds binding to distinct pockets on GDP‐bound Ras has renewed the view druggability this crucial cancer driver. However, origin these pockets, which are not readily formed in crystal structure absence compounds, is yet unclear. Herein, we explored intrinsic flexibility Ras⋅GDP microsecond millisecond timescales using relaxation‐based NMR experiments, and identified substantial slow dynamics with τ ex 34 μs at 5 °C, maps regions showing a high...
The construction industry is the lifeblood of national economy; thereby, to some extent, green transformation representative industrialization levels modern construction, especially in China. Based on panel data A-share listed companies China’s from 2014 2019, this work studies influence mechanism political connection corporate innovation by establishing a multiple regression model, analyzes realization path social responsibility as an mediating variable impact connections innovation, and...
Abstract High-throughput methods for single cell copy number sequencing have enabled the profiling of thousands cells in parallel, yet there remains a significant bottleneck data analysis. Here we present CopyKit, comprehensive set computational pre-processing and analysis to resolve clonal substructure reconstruct genetic lineages tumors. We performed DNA 2977 from multiple spatial regions two liver metastasis 7365 three primary tumors with matched metastatic tissues. In metastases, CopyKit...
To understand how brain regions form and work, it is important to explore the spatially variable genes (SVGs) enriched in specific during development. Spatial transcriptomics techniques provide opportunity select SVGs high-throughput way. However, previous methods neglected ranking order combinatorial effect of SVGs, making them difficult automatically high-priority from spatial data. Here, we proposed a novel computational pipeline, called SVGbit, rank individual for marker selection...
Abstract Background Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy with five-year survival rate of approximately 10%. Genetic mutations are pivotal drivers in PDAC pathogenesis, but recent investigations also revealed the involvement non-genetic alterations disease development. In this study, we undertook multi-omics approach, encompassing ATAC-seq, RNA-seq, ChIP-seq, and Hi-C methodologies, to dissect gene expression arising from changes chromosome accessibility chromatin...