A5016997178- Nerve injury and regeneration
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Neurogenesis and neuroplasticity mechanisms
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Nerve Injury and Rehabilitation
- Hydrogels: synthesis, properties, applications
- Graphene and Nanomaterials Applications
- Infective Endocarditis Diagnosis and Management
- Spinal Cord Injury Research
- Streptococcal Infections and Treatments
- Liver physiology and pathology
- Antimicrobial Resistance in Staphylococcus
- Surgical Sutures and Adhesives
- Virus-based gene therapy research
- Cellular Mechanics and Interactions
- Ultrasound Imaging and Elastography
- Silk-based biomaterials and applications
- biodegradable polymer synthesis and properties
- Facial Rejuvenation and Surgery Techniques
- Advanced MRI Techniques and Applications
- Neonatal and Maternal Infections
University Hospital of Zurich
2024
University of Zurich
2023-2024
Stanford University
2016-2023
Klinikum Magdeburg
2019
University Hospital Magdeburg
2019
Bioengineering Center
2017
National Center for Genetic Engineering and Biotechnology
2017
John Wiley & Sons (United States)
2017
Hudson Institute
2017
Washington University in St. Louis
2011-2016
Transplantation of patient-derived Schwann cells is a promising regenerative medicine therapy for spinal cord injuries; however, therapeutic efficacy compromised by inefficient cell delivery. We present materials-based strategy that addresses three common causes transplanted death: (i) membrane damage during injection, (ii) leakage from the injection site, and (iii) apoptosis due to loss endogenous matrix. Using protein engineering peptide-based assembly, we designed injectable hydrogels...
The use of growth factors, such as glial cell line-derived neurotrophic factor (GDNF), for the treatment peripheral nerve injury has been useful in promoting axon survival and regeneration. Unfortunately, finding a method that delivers appropriate spatial temporal release profile to promote functional recovery proven difficult. Some methods result burst profiles too short remain effective over regeneration period; however, prolonged exposure GDNF can axonal entrapment at site release. Thus,...
Bioactive glasses have recently been shown to promote regeneration of soft tissues by positively influencing tissue remodeling during wound healing. We were interested determine whether bioactive the potential for use in treatment peripheral nerve injury. In these experiments, degradable borate glass was fabricated into rods and microfibers. To study compatibility with neurons, embryonic chick dorsal root ganglia (DRG) cultured different forms glass. Cell viability measured no media exchange...
Abstract Over the past decade, it has been increasingly recognized that both chemical and mechanical properties of scaffolds influence neural cell behavior, ranging from growth to differentiation migration. However, are difficult control for in design nerve regeneration, as change over time most biologically derived scaffolds. The focus this project was examine how a nondegradable scaffold, poly(ethylene glycol) (PEG) gels, influenced behavior. Low concentration PEG 3, 4, or 5% PEG, with...
In the last decade, numerous growth factors and biomaterials have been explored for treatment of myocardial infarction (MI). While pre-clinical studies demonstrated promising results, clinical trials disappointing inconsistent, likely due to poor translatability. present study, we investigate a potential regenerative therapy consisting protein-engineered dimeric fragment hepatocyte factor (HGFdf) encapsulated in shear-thinning, self-healing, bioengineered hydrogel (SHIELD). We hypothesized...
Abstract Within a 3D environment, the chemical and mechanical properties of scaffold can significantly influence nerve behavior. How these with cells is important for optimizing neurite extension within scaffold. The purpose this study was to investigate effect low concentration poly(ethylene glycol) (PEG) added laminin on growth dissociated dorsal root ganglia. Because its high affinity adhesion ability promote extension, conjugated PEG chain, as well mixed in gel, at various concentrations...
Group A streptococcal (GAS; aka Streptococcus pyogenes) meningitis is a fulminant disease associated with high morbidity and mortality. To elucidate the mechanisms underlying invasiveness of GAS in meningitis, we compared isolates derived from five cases to otitis colonizing isolates. We did not observe differences adherence invasion human brain microvascular endothelial cells, virulence factors activity, or barrier disruption. Whole genome sequencing reveal particular traits. Most patients...
Synthetic nerve guidance conduits (NGCs) offer an alternative to harvested grafts for treating peripheral injury (PNI). NGCs have been made from both naturally derived and synthesized materials. While materials typically increased capacity bioactivity, better material control, including tunability reproducibility. Protein engineering is strategy that can bridge the benefits of these two classes by designing cell-responsive are also systematically tunable consistent. Here, we tested a...
Damage to the recurrent laryngeal nerve (RLN) is highly detrimental voice, swallow, and cough. The optimal method for reconstitution of a gap after injury unknown.To evaluate multiple methods RLN reconstruction.This study used an established canine model examine purpose-bred, conditioned, female, 20-kg mongrel hounds at Washington University. A total 32 dogs were examined, with 63 experiments performed.Surgical transection or excision reconstruction by methods.Six months repair, adductor...
Glial cell-line-derived neurotrophic factor (GDNF) is a potent known to enhance motor nerve regeneration following its delivery. However, recent studies have determined that extended GDNF delivery regenerating axons can entrap at the site of This entrapment leads reduced available reinnervate muscle. To address this issue, we designed cell-based expression system temporally regulate protein using an inducible gene excision mechanism prevent expression. design for regulation expression,...
Abstract The study of exosomes has attracted worldwide research due to its ability modulate cellular activities, especially in the progression cancer. Exosomes are membrane vesicles that can deliver lipids, proteins, and nucleic acids from one cell another through fusion, providing a unique form cell-to-cell communication. It been shown KRAS G12V oncoprotein detected inside various pancreatic cancer derived exosomes. Our goal for this was examine effect oncoprotein, isolated THP-1 line...
Schwann cells (SCs) are known to provide chemical and physical cues enhance regeneration following peripheral nerve injury, yet it remains be shown the effect that specific SC phenotypes (motor or sensory) have on axonal functional recovery. In particular, difference between motor sensory may encourage guidance in cell transplantation therapies. Thus, goal of this study was determine whether phenotypically matched combinations neurons SCs stimulate greater neurite extension over mismatched...
AMERICAN SOCIETY OF PLASTIC SURGEONS & RECONSTRUCTIVE SURGERY PRS GLOBAL OPEN ASPS EDUCATION NETWORK
Abstract Group A streptococcal (GAS, aka Streptococcus pyogenes ) meningitis is a fulminant disease associated with high morbidity and mortality. To elucidate the mechanisms underlying invasiveness of GAS in meningitis, we compared isolates derived from five cases to otitis colonizing isolates. We did not observe differences adherence invasion human brain microvascular endothelial cells, virulence factors activity or barrier disruption. Whole genome sequencing reveal particular traits. Most...
Abstract Cervical damage is the most prevalent type of spinal cord injury clinically, although few preclinical research studies focus on this anatomical region injury. Here we present a combinatorial therapy composed custom-engineered, injectable hydrogel and human induced pluripotent stem cell (iPSC)-derived deep cortical neurons. The biomimetic has modular design that includes protein-engineered component to allow customization cell-adhesive peptide sequence synthetic polymer gel...