A5036692197- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Nerve injury and regeneration
- Cellular Mechanics and Interactions
- Connective tissue disorders research
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Tendon Structure and Treatment
- Elasticity and Material Modeling
- Nerve Injury and Rehabilitation
- Extracellular vesicles in disease
- Peripheral Artery Disease Management
- Spinal Cord Injury Research
- Angiogenesis and VEGF in Cancer
- Cardiovascular Function and Risk Factors
- Cardiac Valve Diseases and Treatments
- RNA Interference and Gene Delivery
- Reconstructive Surgery and Microvascular Techniques
- Digestive system and related health
Stanford University
2019-2023
Palo Alto University
2021
Transplantation of patient-derived Schwann cells is a promising regenerative medicine therapy for spinal cord injuries; however, therapeutic efficacy compromised by inefficient cell delivery. We present materials-based strategy that addresses three common causes transplanted death: (i) membrane damage during injection, (ii) leakage from the injection site, and (iii) apoptosis due to loss endogenous matrix. Using protein engineering peptide-based assembly, we designed injectable hydrogels...
Human intestinal organoids from primary human tissues have the potential to revolutionize personalized medicine and preclinical gastrointestinal disease models. A tunable, fully defined, designer matrix, termed hyaluronan elastin-like protein (HELP) is reported, which enables formation, differentiation, passaging of adult tissue-derived, epithelial-only organoids. HELP encapsulation dissociated patient-derived cells, then undergo proliferation formation enteroids, spherical structures with...
Abstract Mechanical cues from the extracellular matrix (ECM) regulate vascular endothelial cell (EC) morphology and function. Since naturally derived ECMs are viscoelastic, cells respond to viscoelastic matrices that exhibit stress relaxation, in which a cell‐applied force results remodeling. To decouple effects of relaxation rate substrate stiffness on EC behavior, we engineered elastin‐like protein (ELP) hydrogels dynamic covalent chemistry (DCC) was used crosslink hydrazine‐modified ELP...
Heart disease is the leading cause of death globally, and delivery therapeutic cargo a promising treatment. However, retention very low. Injectable gels may help improve cargo.
Hydrogels with encapsulated cells have widespread biomedical applications, both as tissue-mimetic 3D cultures in vitro and tissue-engineered therapies vivo. Within these hydrogels, the presentation of cell-instructive extracellular matrix (ECM)-derived ligands stiffness are critical factors known to influence numerous cell behaviors. While individual ECM biopolymers can be blended together alter ligands, this typically results hydrogels a range mechanical properties. Synthetic systems that...
Synthetic nerve guidance conduits (NGCs) offer an alternative to harvested grafts for treating peripheral injury (PNI). NGCs have been made from both naturally derived and synthesized materials. While materials typically increased capacity bioactivity, better material control, including tunability reproducibility. Protein engineering is strategy that can bridge the benefits of these two classes by designing cell-responsive are also systematically tunable consistent. Here, we tested a...
Myocardial infarction ( MI ) is a leading cause of cardiovascular disease in the U.S. and abroad. Transient delivery stromal cell-derived factor 1 SDF-1 α either through protein-encoding genes or protein itself has demonstrated improved cardiac function preclinical models. However, therapy suffers from two limitations: (1) poor retention when injected into contracting myocardium, (2) rapid degradation protein, which requires prolonged activity to be therapeutically effective. To mitigate...
Abstract Cervical damage is the most prevalent type of spinal cord injury clinically, although few preclinical research studies focus on this anatomical region injury. Here we present a combinatorial therapy composed custom-engineered, injectable hydrogel and human induced pluripotent stem cell (iPSC)-derived deep cortical neurons. The biomimetic has modular design that includes protein-engineered component to allow customization cell-adhesive peptide sequence synthetic polymer gel...
Abstract Heart disease is the leading cause of death globally, and delivery therapeutic cargo ( e.g. cells, proteins, drugs) through direct injection into myocardium a promising clinical intervention. However, retention deliverables to contracting low, with as much 60 - 90% payload being lost within 24 hours. Commercially-available injectable hydrogels, including Matrigel, have been hypothesized increase retention, but not yielded significant improvements in quantified analyses. Here, we...
Introduction: Endothelial cells can improve blood perfusion in diseased vessels; however, direct injection of significantly decreases their survival and functionality for angiogenesis. To address these limitations, we study a family engineered extracellular matrices with tunable biochemical biomechanical cues enhanced improved angiogenic behavior ECs. Materials & Methods: Engineered hydrogels, termed ELP-PEG, consists two components hydrazine-modified elastin-like protein (ELP-HYD) an...