A5056065136- Cellular Mechanics and Interactions
- Electrospun Nanofibers in Biomedical Applications
- Connective tissue disorders research
- 3D Printing in Biomedical Research
- Tendon Structure and Treatment
- Biomedical Ethics and Regulation
- Pluripotent Stem Cells Research
- Theatre and Performance Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Bone and Dental Protein Studies
- Cinema and Media Studies
- Cardiovascular Function and Risk Factors
- Cardiac Valve Diseases and Treatments
- Aortic aneurysm repair treatments
- Dental Trauma and Treatments
- Tissue Engineering and Regenerative Medicine
- Anatomy and Medical Technology
- Advanced biosensing and bioanalysis techniques
- Cancer Research and Treatments
- Hip disorders and treatments
- Hydrogels: synthesis, properties, applications
- Cell Image Analysis Techniques
- Orthopaedic implants and arthroplasty
- Additive Manufacturing and 3D Printing Technologies
- Artistic and Creative Research
Stanford University
2022-2025
Pontificia Universidad Católica de Chile
2022
Navarro College
2022
Centro de Investigação em Artes e Comunicação
2021
Universidade de São Paulo
2021
University of Michigan
2021
Three-dimensional bioprinting has emerged as a promising tool for spatially patterning cells to fabricate models of human tissue. Here, we present an engineered bioink material designed have viscoelastic mechanical behavior, similar that living This is cross-linked through dynamic covalent bonds, reversible bond type allows cellular remodeling over time. Viscoelastic materials are challenging use inks, one must tune the kinetics cross-links allow both extrudability and long-term stability....
Human-induced pluripotent stem cells (hiPSCs) have emerged as a promising in vitro model system for studying neurodevelopment. However, current models remain limited their ability to incorporate tunable biomechanical signaling cues imparted by the extracellular matrix (ECM). The native brain ECM is viscoelastic and stress-relaxing, exhibiting time-dependent response an applied force. To recapitulate remodelability of neural ECM, we developed family protein-engineered hydrogels that exhibit...
Abstract Mechanical cues from the extracellular matrix (ECM) regulate vascular endothelial cell (EC) morphology and function. Since naturally derived ECMs are viscoelastic, cells respond to viscoelastic matrices that exhibit stress relaxation, in which a cell‐applied force results remodeling. To decouple effects of relaxation rate substrate stiffness on EC behavior, we engineered elastin‐like protein (ELP) hydrogels dynamic covalent chemistry (DCC) was used crosslink hydrazine‐modified ELP...
Dynamic covalent chemistry (DCC) crosslinks can form hydrogels with tunable mechanical properties permissive to injectability and self-healing. However, not all transient are easily extrudable. For this reason, two additional design parameters must be considered when formulating DCC-crosslinked hydrogels: 1) degree of functionalization (DoF) 2) polymer molecular weight (MW). To investigate these parameters, comprised recombinant biopolymers: a hyaluronic acid (HA) modified benzaldehyde an...
Mechanically tunable hydrogels are attractive platforms for 3D cell culture, as hydrogel stiffness plays an important role in behavior. Traditionally, has been controlled through altering either the polymer concentration or stoichiometry between crosslinker reactive groups. Here, alternative strategy based upon tuning hydrophilicity of elastin-like protein (ELP) is presented. ELPs undergo a phase transition that leads to aggregation at increasing temperatures. It hypothesized this...
Hydrogels with encapsulated cells have widespread biomedical applications, both as tissue-mimetic 3D cultures in vitro and tissue-engineered therapies vivo. Within these hydrogels, the presentation of cell-instructive extracellular matrix (ECM)-derived ligands stiffness are critical factors known to influence numerous cell behaviors. While individual ECM biopolymers can be blended together alter ligands, this typically results hydrogels a range mechanical properties. Synthetic systems that...
Blending sacrificial, cell-laden microgels with structural, UV-crosslinkable produces a family of modular bioinks tunable void fractions that influence cellular morphology while maintaining depth-independent cell distribution.
Abstract Three‐dimensional cell encapsulation has rendered itself a staple in the tissue engineering field. Using recombinantly engineered, biopolymer‐based hydrogels to encapsulate cells is especially promising due enhanced control and tunability it affords. Here, we describe detail synthesis of our hyaluronan (i.e., hyaluronic acid) elastin‐like protein (HELP) hydrogel system. In addition validating efficacy synthetic process, also demonstrate modularity HELP Finally, show that can be...
Myocardial infarction ( MI ) is a leading cause of cardiovascular disease in the U.S. and abroad. Transient delivery stromal cell-derived factor 1 SDF-1 α either through protein-encoding genes or protein itself has demonstrated improved cardiac function preclinical models. However, therapy suffers from two limitations: (1) poor retention when injected into contracting myocardium, (2) rapid degradation protein, which requires prolonged activity to be therapeutically effective. To mitigate...
The successful treatment of hip dysplasia consists achieving a concentric reduction and avoiding residual dysplasia. One the essential factors is early diagnosis treatment.Evaluate relationship between age at initiation presence one year age.Prognostic retrospective study. Patients with treated Pavlik harness in tertiary healthcare center were selected. Residual dyspla sia was defined as an acetabular index greater than 28 degrees age. An association initiation, bilaterality, more 36...
Introduction: Endothelial cells (ECs) can improve blood perfusion in diseased vessels; however, direct injection of significantly decreases their survival and functionality for angiogenesis. To address these limitations, we study a family engineered extracellular matrices with tunable biochemical biomechanical cues enhanced improved angiogenic behavior primary human ECs. Hypothesis: We hypothesize that the properties ELP-PEG hydrogels will modulate EC function vitro. Methods: Engineered...