A5028831267- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Nerve injury and regeneration
- Electrospun Nanofibers in Biomedical Applications
- Memory and Neural Mechanisms
- Mesenchymal stem cell research
- Neuroscience and Neuropharmacology Research
- Elasticity and Material Modeling
- Neonatal and fetal brain pathology
- Nerve Injury and Rehabilitation
- Shoulder Injury and Treatment
- Biochemical effects in animals
- Olfactory and Sensory Function Studies
- Vitamin C and Antioxidants Research
- Cellular Mechanics and Interactions
- Sleep and Wakefulness Research
- Biomedical Ethics and Regulation
- Orthopedic Surgery and Rehabilitation
- Tendon Structure and Treatment
- Health, Environment, Cognitive Aging
- Traumatic Brain Injury Research
- Tryptophan and brain disorders
- Adipose Tissue and Metabolism
- Birth, Development, and Health
- Spinal Cord Injury Research
Stanford University
2016-2025
University of Massachusetts Lowell
2012-2015
Czech Academy of Sciences, Institute of Physiology
2009-2012
Czech Academy of Sciences
2012
Transplantation of patient-derived Schwann cells is a promising regenerative medicine therapy for spinal cord injuries; however, therapeutic efficacy compromised by inefficient cell delivery. We present materials-based strategy that addresses three common causes transplanted death: (i) membrane damage during injection, (ii) leakage from the injection site, and (iii) apoptosis due to loss endogenous matrix. Using protein engineering peptide-based assembly, we designed injectable hydrogels...
Of the 1.5 million emergency room visits each year in United States due to flexor tendon injuries hand, over 30-40% result peritendinous adhesions which can limit range of motion (ROM) and severely impact an individual's quality life. Adhesions are fibrous scar-like tissues form between adjacent body response injury, inflammation, or during normal healing following surgery. Currently, there is no widespread solution for adhesion prevention delicate space digit while allowing a patient full...
Environmental stimulation and increased social interactions stimulate cognitive performance, while decrease in these parameters can exacerbate decline as a function of illness, injury, or age. We examined the impact environmental on performance healthy adult C57B1/6J mice. Mice were housed for 1 month individually groups three (to prevent allow interaction) either standard environment (SE) an enlarged cage containing nesting material items classically utilized to exploration activity...
Heart disease is the leading cause of death globally, and delivery therapeutic cargo a promising treatment. However, retention very low. Injectable gels may help improve cargo.
Synthetic nerve guidance conduits (NGCs) offer an alternative to harvested grafts for treating peripheral injury (PNI). NGCs have been made from both naturally derived and synthesized materials. While materials typically increased capacity bioactivity, better material control, including tunability reproducibility. Protein engineering is strategy that can bridge the benefits of these two classes by designing cell-responsive are also systematically tunable consistent. Here, we tested a...
Recovery following Traumatic Brain Injury (TBI) can vary tremendously among individuals. Lifestyle injury, including differential social interactions, may modulate the extent of secondary injury TBI. To examine this possibility under controlled conditions, closed head (CHI) was induced in C57Bl6 mice using a standardized weight drop device after which were either housed isolation or with their original cagemates ("socially-housed") for 4 weeks. CHI transiently impaired novel object...
Alzheimer's disease encompasses multiple risk factors; convergence may be necessary for clinical manifestation. Mice received a complete diet or one lacking folate and vitamin E containing iron as pro-oxidant, in standard environment (SE) large cage objects to stimulate expl oration/activity (enriched environment; EE). declined maze navigation on the deficient versus SE but not EE. demonstrated superior performance EE SE. The reduced brain lipid protein oxidation. These findings suggest that...
Abstract Cervical spinal cord injuries (SCI) sever and permanently disrupt sensorimotor neural circuitry. Restoring connectivity within the damaged circuitry is critical to improving function. Herein we report robust regeneration of severed in a rat SCI model following transplantation human induced pluripotent cells differentiated towards deep cortical neuron lineage (iPSC-DCNs). In vivo , iPSC-DCNs: (1) integrated extended axons caudal targets, (2) reversed pathophysiology, (3) promoted...
Abstract Cervical damage is the most prevalent type of spinal cord injury clinically, although few preclinical research studies focus on this anatomical region injury. Here we present a combinatorial therapy composed custom-engineered, injectable hydrogel and human induced pluripotent stem cell (iPSC)-derived deep cortical neurons. The biomimetic has modular design that includes protein-engineered component to allow customization cell-adhesive peptide sequence synthetic polymer gel...
Nutrition, environment, and social interactions have been shown to influence cognitive decline in preclinical clinical studies. Oxidative stress often leads decreased cognition cortical atrophy by overwhelming endogenous antioxidant systems. In rodents, impaired was linked diets deficient antioxidants but high pro-oxidants while performance improved following maintenance on antioxidant-supplemented diets. Longitudinal studies demonstrated that strong networks, exercise, mentally-stimulating...
Abstract Heart disease is the leading cause of death globally, and delivery therapeutic cargo ( e.g. cells, proteins, drugs) through direct injection into myocardium a promising clinical intervention. However, retention deliverables to contracting low, with as much 60 - 90% payload being lost within 24 hours. Commercially-available injectable hydrogels, including Matrigel, have been hypothesized increase retention, but not yielded significant improvements in quantified analyses. Here, we...