A5076100549- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Carcinogens and Genotoxicity Assessment
- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
- Adipokines, Inflammation, and Metabolic Diseases
- DNA and Nucleic Acid Chemistry
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Acute Myeloid Leukemia Research
- Genetic factors in colorectal cancer
- Hedgehog Signaling Pathway Studies
- Adipose Tissue and Metabolism
- PARP inhibition in cancer therapy
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Fungal and yeast genetics research
- Acute Lymphoblastic Leukemia research
- Mitochondrial Function and Pathology
- Cardiovascular Disease and Adiposity
Universidad San Pablo CEU
2021
Universidad Autónoma de Madrid
2003-2013
Consejo Superior de Investigaciones Científicas
2005-2013
Centro de Biología Molecular Severo Ochoa
2003-2012
Institute of Molecular Biology
2012
We describe a second primase in human cells, PrimPol, which has the ability to start DNA chains with deoxynucleotides unlike regular primases, use exclusively ribonucleotides. Moreover, PrimPol is also polymerase tailored bypass most common oxidative lesions DNA, such as abasic sites and 8-oxoguanine. Subcellular fractionation immunodetection studies indicated that present both nuclear mitochondrial compartments. activity detectable lysates from mouse cells but absent mitochondria derived...
The transcription factor Sp2 is essential for early mouse development and proliferation of embryonic fibroblasts in culture. Yet its mechanisms action target genes are largely unknown. In this study, we have combined RNA interference, vitro DNA binding, chromatin immunoprecipitation sequencing global gene-expression profiling to investigate the role cellular functions, define sites identify regulated by Sp2. We show that important it binds GC-boxes occupies proximal promoters vital processes...
Polymerase micro (Polmicro) is an error-prone, DNA-directed DNA polymerase that participates in non-homologous end-joining (NHEJ) repair. In vivo, Polmicro deficiency results impaired Vkappa-Jkappa recombination and altered somatic hypermutation centroblast development. Polmicro(-/-) mice, hematopoietic development was defective several peripheral bone marrow (BM) cell populations, with about a 40% decrease BM number affected lineages. Hematopoietic progenitors were reduced both expansion...
The molecular complexes involved in the nonhomologous end-joining process that resolves recombination-activating gene (RAG)-induced double-strand breaks and results V(D)J rearrangements vary during mammalian ontogeny. In mouse, first immunoglobulin emerge midgestation periods, but their repertoires have not been analyzed detail. We decided to study postgastrulation DJ(H) joints compare them with those present later life. embryo differed from observed perinatal life by presence of short...
As predicted by the amino acid sequence, purified protein coded Schizosaccharomyces pombe SPAC2F7.06c is a DNA polymerase (SpPol4) whose biochemical properties resemble those of other X family (PolX) members. Thus, this new PolX template-dependent, polymerizes in distributive manner, lacks detectable 3'-->5' proofreading activity and its preferred substrates are small gaps with 5'-phosphate group. Similarly to Polmu, SpPol4 can incorporate ribonucleotide (rNTP) into primer DNA. However, it...
Background DNA polymerase lambda (Polλ) is a repair polymerase, which likely plays role in base excision (BER) and non-homologous end joining (NHEJ) of double-strand breaks (DSB). Principal Findings Here, we described novel natural allelic variant human Polλ (hPolλ) characterized by single nucleotide polymorphism (SNP), C/T variation the first codon 438, resulting amino acid change Arg to Trp. In vitro enzyme activity assays purified W438 revealed that it retained both polymerization...
(1) Background: Pleiotrophin preserves insulin sensitivity, regulates adipose tissue lipid turnover and plasticity, energy metabolism thermogenesis. The aim of this study was to determine the role pleiotrophin in hepatic metabolic crosstalk between liver brown white (AT) a high-fat diet-induced (HFD) obesity mice model. (2) Methods: We analyzed circulating variables, (hepatic content mRNA expression), AT thermogenesis (UCP-1 expression) periovarian browning (brown adipocyte markers...
Polµ is the only DNA polymerase equipped with template-directed and terminal transferase activities. also able to accept distortions in both primer template strands, resulting misinsertions extension of realigned mismatched terminus. In this study, we propose a model for human Polµ-mediated dinucleotide expansion as function sequence context. model, requires an initial dislocation, that must be subsequently stabilized, generate large expansions at different 5′-P-containing substrates,...
Background: DNA polymerase lambda (Poll) is a repair polymerase, which likely plays role in base excision (BER) and non-homologous end joining (NHEJ) of double-strand breaks (DSB).Principal Findings: Here, we described novel natural allelic variant human Poll (hPoll) characterized by single nucleotide polymorphism (SNP), C/T variation the first codon 438, resulting amino acid change Arg to Trp.In vitro enzyme activity assays purified W438 revealed that it retained both polymerization...