A5085607917- Photoreceptor and optogenetics research
- Micro and Nano Robotics
- Microtubule and mitosis dynamics
- Acoustic Wave Resonator Technologies
- Mechanical and Optical Resonators
- Protist diversity and phylogeny
- Force Microscopy Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Protein Structure and Dynamics
- Lipid Membrane Structure and Behavior
- ATP Synthase and ATPases Research
- Advanced Fluorescence Microscopy Techniques
- Microbial Inactivation Methods
- Cellular Mechanics and Interactions
- Ultrasonics and Acoustic Wave Propagation
- Advanced MEMS and NEMS Technologies
- Hemoglobin structure and function
- Semiconductor materials and devices
- Copper Interconnects and Reliability
- Bacteriophages and microbial interactions
- Electrohydrodynamics and Fluid Dynamics
- Analytical Chemistry and Sensors
- Magnetic properties of thin films
- Cardiac electrophysiology and arrhythmias
- Microfluidic and Bio-sensing Technologies
Kindai University
2019-2024
Kyoto University
2012-2023
Kyoto University Institute for Chemical Research
2020-2023
Kyoto Bunkyo University
2020
Material Sciences (United States)
2013-2018
Osaka University
2004-2013
Advanced Research Institute
2013
Japan Science and Technology Agency
2009-2012
Hosei University
2012
Disaster Reduction and Human Renovation Institution
2010-2011
We develop a highly sensitive quartz crystal microbalance (QCM) biosensor with fundamental resonance frequency of 170 MHz. A naked AT-cut plate 9.7 μm thick is set in sensor cell. Its shear vibration excited by the line wire, and signals are detected other achieving noncontacting measurement frequency. The mass sensitivity MHz QCM 15 pg/(cm2 Hz), which better than that conventional 5 3 orders magnitude. high confirmed detecting human immunoglobulin G (hIgG) via Staphylococcus protein...
Liposomes encapsulating cytoskeletons have drawn much recent attention to develop an artificial cell-like chemical-machinery; however, as far we know, there has been no report showing isothermally reversible morphological changes of liposomes containing because the sets various regulatory factors, that is, their interacting proteins, are required control state every reaction system cytoskeletons. Here focused on hydrostatic pressure polymerization microtubules (MTs) within cell-sized giant...
The energy difference between the oxide and substrate Si 2p peaks for silicon oxide/Si structures increases with thickness. dependence of shift on thickness almost disappears deposition a thin palladium overlayer, because avoidance surface charging effect due to photoemission nearly constant resulting from extra atomic relaxation. true chemical layers thicker than 2 nm is determined be ∼3.8 eV. For energy, relaxation are dominant thinner ∼2 ∼4 nm, respectively. In intermediate region, both...
In this paper, we developed a 55-MHz wireless−electrodeless quartz crystal microbalance (QCM) and systematically studied the effects of flow rate on sensitivity to detection proteins affinity between biomolecules evaluated by injection system. Brownian motion in liquid suggests low probability meeting, convection effect plays an important role cell The wireless was isolated QCM cell, rates 50 1000 μL/min were used for monitoring binding reactions human immunoglobulin G Staphylococcus aureus...
We develop the wireless-electrodeless multichannel quartz-crystal microbalance (QCM) biosensor using quartz plates of slightly different thicknesses. Their shear vibrations are simultaneously excited and detected by a pair antenna wires to perform noncontacting measurement. fundamental resonance frequencies between 43 55 MHz, at up 10 channels measured in liquids. Owing high affinity naked surfaces for proteins, we immobilized various receptor proteins on nonspecifically antigen−antibody...
ABSTRACT The bacterial flagellar motor is a reversible rotary machine that rotates left-handed helical filament, allowing bacteria to swim toward more favorable environment. direction of rotation reverses from counterclockwise (CCW) clockwise (CW), and vice versa, in response input the chemotaxis signaling circuit. CW normally caused by binding phosphorylated regulator CheY (CheY-P), strains lacking are typically locked CCW rotation. detailed mechanism switching remains unresolved because it...
Hydrostatic pressure is one of the physical stimuli that characterize environment living matter. Many microorganisms thrive under high and may even physically or geochemically require this extreme environmental condition. In contrast, application detrimental to most life on Earth; especially organisms ambient conditions. To study mechanism how things adapt high-pressure conditions, it necessary monitor directly organism interest various Here, we report a miniature chamber for microscopy. The...
Abstract Aquifex aeolicus is a hyperthermophilic, hydrogen-oxidizing and carbon-fixing bacterium that can grow at temperatures up to 95 °C. A. has an almost complete set of flagellar genes are conserved in bacteria. Here we observed polar flagellum swim with speed 90 μm s −1 85 We expressed the mot ( motA motB ), which encode torque generating stator proteins motor, corresponding nonmotile mutant Escherichia coli . Its motility was slightly recovered by expression MotA chimeric MotB whose...
Summary We describe the construction and characterization of a laser‐line‐scanning microscope capable detection broad fluorescence spectra with resolution 1 nm. A near‐infrared femtosecond pulse train at 800 nm was illuminated on line (one lateral axis, denoted as X axis) in specimen by resonant scanning mirror oscillating 7.9 kHz, total multi‐photon–induced from linear region focused slit an imaging polychromator. An electron‐multiplying CCD camera used to resolve different colours...
Fluorescent protein-based indicators for intracellular environment conditions such as pH and ion concentrations are commonly used to study the status dynamics of living cells. Despite being an important factor in many biological processes, development indicator physicochemical state water, pressure, viscosity temperature, however, has been neglected. We here found a novel mutation that dramatically enhances pressure dependency yellow fluorescent protein (YFP) by inserting several glycines...
High pressure below 100 MPa interferes inter-molecular interactions without causing denaturation of proteins. In Escherichia coli, the binding chemotaxis signaling protein CheY to flagellar motor FliM induces reversal rotation. Using molecular dynamics (MD) simulations and parallel cascade selection MD (PaCS-MD), we show that high increases water density in first hydration shell considerably penetration into CheY-FliM interface. PaCS-MD enabled us observe pressure-induced dissociation...