Zinc selenide nanoparticles (ZnSe NPs) were synthesized in the cavity of cage-shaped protein apoferritin by designing a slow chemical reaction system, which employs tetraaminezinc ion and selenourea. The synthesis ZnSe NPs was realized spatially selective manner from an aqueous solution, cores formed almost all cavities with little bulk precipitation. Three factors are found to be important for NP cavity: (1) threefold channel, selectively introduces zinc into cavity, (2) internal potential,...

Abstract Tendon self-renewal is a rare occurrence because of the poor vascularization this tissue; therefore, reconstructive surgery using autologous tendon often performed in severe injury cases. However, post-surgery re-injury rate relatively high, and collection tendons leads to muscle weakness, resulting prolonged rehabilitation. Here, we introduce an induced pluripotent stem cell (iPSC)-based technology develop therapeutic option for injury. First, derived tenocytes from human iPSCs by...

The early detection of HER2 (human epidermal growth factor receptor 2) status in breast cancer patients is very important for the effective implementation anti-HER2 antibody therapy. Recently, detections using conjugated quantum dots (QDs) have attracted much attention. QDs are a new class fluorescent materials that superior properties such as high brightness, resistance to photo-bleaching, and multi-colored emission by single-light source excitation. In this study, we synthesized three...

Abstract Fluorescent proteins have been widely used in biology because of their compatibility and varied applications living specimens. are often undesirably sensitive to intracellular conditions such as pH ion concentration, generating considerable issues at times. However, harnessing these intrinsic sensitivities can help develop functional probes. In this study, we found that the fluorescence yellow fluorescent protein (YFP) depends on concentration solution dependence be enhanced by...

Surface modification of flow-diverting stents has been explored to reduce thrombus-related complications that may arise under clinical use. This study investigated the thromboresistant properties flow redirection endoluminal device (FRED) X, a diverter treated with copolymer poly(2-methoxyethyl acrylate) (PMEA; X Technology).The performance FRED, FRED and Pipeline Flex Shield Technology (sPED) was evaluated in an vitro blood loop model. Blood activation level assessed by concentration...

To use quantum dots (QDs) as fluorescent probes for receptor imaging, QD surface should be modified with biomolecules such antibodies, peptides, carbohydrates, and small-molecule ligands receptors. Among these QDs, antibody conjugated QDs are the most promising probes. There many kinds of coupling reactions that can used preparing QDs. Most reactions, however, non-selective time-consuming. In this paper, we report a facile method imaging. We ProteinA an adaptor protein binding to By using...

Abstract Au2S nanoparticles (NPs) were prepared in the cavity of cage-shaped protein, apoferritin. Apoferritin has a cavity, 7 nm diameter, and diameter fabricated NPs is about same size with dispersion was small. Although generally insoluble water, our can be dissolved over 100 mg/mL water due to water-soluble protein shell. This solubility will make it possible place construct nanostructures made NPs.

This paper describes a path tracking method for power wheeled steering mobile robots (PWS). In the traditional moving robot along desired path, state variables of (position and orientation) are controlled. But we think that only position must be controlled in because orientation comes close to correct value motion path. We propose new based on this idea show performance algorithm by simulation experimental results.

The denaturation of recombinant horse L-chain apoferritin (rLF), which is composed 24 subunits, in acidic solution was studied. Using two rLF mutants, lacking four (Fer4) or eight (Fer8) N-terminal amino acid residues, the effect residues on protein's stability investigated. Of mutants and wild-type rLF, tertiary secondary structures Fer8 were found to be most sensitive an environment. protein dissociated easily into subunit dimers at below pH 2.0. Comparing crystal mutant proteins, deletion...

Fluorescent protein-based indicators for intracellular environment conditions such as pH and ion concentrations are commonly used to study the status dynamics of living cells. Despite being an important factor in many biological processes, development indicator physicochemical state water, pressure, viscosity temperature, however, has been neglected. We here found a novel mutation that dramatically enhances pressure dependency yellow fluorescent protein (YFP) by inserting several glycines...

Deuterium solid-state NMR was used to study the dynamics and molecular structure of serine (Ser) side chains in silk fibroin from Bombyx mori Samia cynthia ricini. Samples were selectively labeled with [3,3-2H2]Ser, 2H powder spectra analyzed by line shape simulation. Two types motion could be characterized quantitatively: one component undergoing a rapid three-site jump (25%) second representing slow exchange between sites unequal occupancies small amplitude libration (75%). From these...

Various biological events including muscle contraction and vesicle transport can be described as a mechanical process. Many of the corresponding proteins are thus required to generate or sense force. Here we describe strain-sensitive yellow fluorescent protein (YFP) recombinant that detect intramolecular strains these experience.

Solid-state deuterium NMR (2H NMR) was used to study the dynamics of tyrosine (Tyr) residue in silk fibroin from Bombyx mori (B. mori) and Samia cynthia ricini (S. c. ricini). Specifically deuterated cocoon obtained by feeding worms with Tyr, labeled either at Cβ carbon ([3,3-2H2]Tyr) or aromatic ring ([3',5'-2H2]Tyr). The 2H spectra [3,3-2H2]Tyr-labeled fibroins showed typical rigid powder patterns, indicating that there is essentially no motion about Cα−Cβ bond axis, both B. S. ricini. In...

Biological effects of sex steroids (estradiol‐17β, E2; progesterone, P; medroxyprogesterone acetate, MPA; Danazol, DZ) and growth factors (epidermal factor, EGF; transforming TGF‐α, β) on migration invasion endometrial adenocarcinoma SNG‐M cells were investigated by haptotactic haptoinvasion assay. The enzymatic degradation the extracellular matrix hy tumor was also examined. Tumor cell along a gradient substratum‐bound fibronectin inhibited 0.1–10 μM MPA DZ, but promoted nM EGF TGF‐a in...

We investigated the effects of epidermal growth factor (EGF) and transforming (TGF)-alpha on migration, invasion matrix metalloproteinase (MMP) expression uterine cervical-carcinoma SKG-IIIb cells, whether these factors affect pyrimidine-nucleoside-phosphorylase (PyNPase) activity 5'-deoxy-5-fluorouridine (5'-dFUrd) sensitivity tumor cells. Tumor-cell migration along a gradient substratum-bound fibronectin into reconstituted basement membrane were stimulated by 0.1 to 100 ng/ml EGF TGF-alpha...

Uterine cervical adenocarcinoma typically is an aggressive neoplasm with a propensity for early invasion and dissemination; however, the regulatory mechanism of invasive activity cells has not been fully understood. In this study, biological effects epidermal growth factor (EGF) transforming (TGF)-α on proteinase expression human OMC-4 were investigated. Tumor cell migration along gradient substratum-bound fibronectin into reconstituted basement membrane stimulated by 0.1–10...

We investigated the effects of epidermal growth factor (EGF) and transforming factor-alpha (TGF-α) on migration, invasion proteinase expression gynecological cultured cancer cells (SKG-IIIb cervical squamous cell carcinoma, OMC-4 adenocarcinoma, SNG-M endometrial adenocarcinoma OMC-3 ovarian adenocarcinoma), whether these factors affect thymidine phosphorylase/platelet-derived endothelial tumor cells. Tumor migration along a gradient substratum-bound fibronectin into reconstituted basement...

Abstract In this study, we developed a mycelial dispersion strain by disrupting the pkac2 gene in white-rot fungus Pleurotus ostreatus. is catalytic subunit of protein kinase A, which regulates several transcription factors related to cell wall synthesis. Liquid cultures Δpkac2 strains showed very high dispersibility and were visibly different from wild-type (WT). Although growth on agar medium was slower than that WT, grew faster liquid culture had approximately twice dry weight WT after 5...

Effects of sex steroids (estradiol-17 beta, E2; progesterone, Prog) and growth factors (epidermal factor, EGF; transforming factor-alpha, TGF-alpha) on invasive activity 5'-deoxy-5-fluorouridine (5'-dFUrd) sensitivity ovarian adenocarcinoma OMC-3 cells were investigated. Tumor cell migration along a gradient substratum-bound fibronectin invasion into reconstituted basement membrane inhibited by 10 microM Prog, but stimulated 0.1-10 nM EGF TGF-alpha in concentration-dependent manner. E2 did...