A5069086844- Pluripotent Stem Cells Research
- T-cell and B-cell Immunology
- Lymphoma Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- 3D Printing in Biomedical Research
- Metabolomics and Mass Spectrometry Studies
- Chronic Lymphocytic Leukemia Research
- Renal and related cancers
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Cancer Immunotherapy and Biomarkers
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Peptidase Inhibition and Analysis
- Barrier Structure and Function Studies
- Neurobiology and Insect Physiology Research
- Ubiquitin and proteasome pathways
- Spider Taxonomy and Behavior Studies
- Hedgehog Signaling Pathway Studies
- Renal cell carcinoma treatment
- Organ Donation and Transplantation
- Neurogenesis and neuroplasticity mechanisms
- Bat Biology and Ecology Studies
Kyoto University
2016-2025
Kyushu University
2025
University of Edinburgh
2023
Italian Aerospace Research Centre
2015-2017
Keio University
2009-2010
Japan Science and Technology Agency
2009-2010
Hokkaido University
2010
Kyoto Institute of Technology
2010
National Cerebral and Cardiovascular Center
2009
Hokkaido University of Science
2004
Abstract Prolonged expression of the CRISPR-Cas9 nuclease and gRNA from viral vectors may cause off-target mutagenesis immunogenicity. Thus, a transient delivery system is needed for therapeutic genome editing applications. Here, we develop an extracellular nanovesicle-based ribonucleoprotein named NanoMEDIC by utilizing two distinct homing mechanisms. Chemical induced dimerization recruits Cas9 protein into nanovesicles, then RNA packaging signal self-cleaving riboswitches tether release...
Rapid progress is being made in mass spectrometry (MS)-based proteomics, yielding an increasing number of larger datasets with higher quality and throughput. To integrate proteomics generated from various projects institutions, we launched a project named jPOST (Japan ProteOme STandard Repository/Database, https://jpostdb.org/) 2015. Its data repository, jPOSTrepo, began operations 2016 has accepted more than 10 TB MS-based the past two years. In addition, have developed new database jPOSTdb...
Novel APOBEC1 target 1 (Nat1) (also known as "p97," "Dap5," and "Eif4g2") is a ubiquitously expressed cytoplasmic protein that homologous to the C-terminal two thirds of eukaryotic translation initiation factor 4G (Eif4g1). We previously showed Nat1-null mouse embryonic stem cells (mES cells) are resistant differentiation. In current study, we found NAT1 eIF4G1 share many binding proteins, such factors eIF3 eIF4A ribosomal proteins. However, did not bind eIF4E or poly(A)-binding which...
Abstract Tendon self-renewal is a rare occurrence because of the poor vascularization this tissue; therefore, reconstructive surgery using autologous tendon often performed in severe injury cases. However, post-surgery re-injury rate relatively high, and collection tendons leads to muscle weakness, resulting prolonged rehabilitation. Here, we introduce an induced pluripotent stem cell (iPSC)-based technology develop therapeutic option for injury. First, derived tenocytes from human iPSCs by...
Proteome analyses of human induced pluripotent stem cells (iPSC) were carried out on a liquid chromatography-tandem mass spectrometry system using meter-scale monolithic silica-C18 capillary columns without prefractionation. Tryptic peptides from five different iPSC lysates and three fibroblast (4 μg each) directly injected onto 200 cm long, 100 μm i.d. column an 8-h gradient was applied at 500 nL/min less than 20 MPa. We identified 98,977 nonredundant tryptic 9510 proteins (corresponding to...
Recent advances have made modeling human small intestines in vitro possible, but it remains a challenge to recapitulate fully their structural and functional characteristics. We suspected interstitial flow within the intestine, powered by circulating blood plasma during embryonic organogenesis, be vital factor. aimed construct an vivo-like multilayered intestinal tissue incorporating into system and, turn, developed micro-small intestine differentiating definitive endoderm mesoderm cells...
The blood-brain barrier (BBB) is composed of four cell populations, brain endothelial cells (BECs), pericytes, neurons, and astrocytes. Its role to precisely regulate the microenvironment through selective substance crossing. Here we generated an in vitro model BBB by differentiating human induced pluripotent stem (hiPSCs) into all populations. When hiPSC-derived populations were co-cultured, (ECs) endowed with features consistent BECs, including a high expression nutrient transporters...
Cellular function and diversity are orchestrated by complex interactions of fundamental biomolecules including DNA, RNA proteins. Technological advances in genomics, epigenomics, transcriptomics proteomics have enabled massively parallel unbiased measurements. Such high-throughput technologies been extensively used to carry out broad, studies, particularly the context human diseases. Nevertheless, a unified analysis genome, epigenome, transcriptome proteome single cell type obtain coherent...
The number of patients requiring dialysis therapy continues to increase worldwide because the lack effective treatments for chronic kidney disease (CKD). Furthermore, no curative acute injury (AKI) have been established. therapeutic effects human induced pluripotent stem cell–derived nephron progenitor cells (hiPSC-NPCs) on AKI reported in mice but not clinically confirmed. There are also reports examining potential hiPSC-NPCs CKD. Although large numbers uniform required cell therapies and...
Cryptococcus neoformans ( Cn ) is a fungal pathogen responsible for cryptococcal meningitis, which accounts 15% of AIDS-related deaths. Recent studies have shown that the absence sterol β-glucosidase (EGCrP2, also known as Sgl1) in significantly attenuates its virulence mouse infection model. However, mechanisms underlying this attenuation remain unclear. In study, we observed significant increase dead cells after 3 days culture SGL1 -deficient sgl1 Δ, KO) at 37°C, compared with wild-type...
Previous studies have suggested that the loss of translation initiation factor eIF4G1 homolog NAT1 induces excessive self-renewability naive pluripotent stem cells (PSCs); yet role in self-renewal and differentiation primed PSCs is still unclear. Here, we generate a conditional knockout use for functional analyses NAT1. Our results show required neural PSCs. In contrast, deficiency pluripotency attenuates to all cell types. We also find involved efficient protein expression an RNA...
We have developed a simple and unbiased method for membrane proteome analysis using cyanocysteine-mediated cleavage in combination with trypsin digestion. In our previous study, application of the trypsin-based phase-transfer surfactants (PTS) protocol provided substantial improvement identification proteome, but task remains challenging, because often generates peptides larger than observable m/z range. Here, we predict computationally that Cys tryptic digestion would be more effective...
ABSTRACT Porphyromonas gingivalis forms communities with antecedent oral biofilm constituent streptococci. P. major fimbriae bind to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) present on the streptococcal surface, and this interaction plays an important role in colonization. This study identified binding domain of Streptococcus oralis GAPDH for fimbriae. S. recombinant (rGAPDH) was digested lysyl endopeptidase. Cleaved fragments rGAPDH were applied a reverse-phase high-pressure liquid...
The effects of transcription factors on the maintenance and differentiation human-induced or embryonic pluripotent stem cells (iPSCs/ESCs) have been well studied. However, importance posttranscriptional regulatory mechanisms, which cause quantitative dissociation mRNA protein expression, has not explored in detail. Here, by combining transcriptome proteome profiling, we identified 228 posttranscriptionally regulated genes with strict upregulation level iPSCs/ESCs. Among them, found 84 were...
Rapid progress in mass spectrometry (MS) has made comprehensive analyses of the proteome possible, but accurate quantification remains challenging. Isobaric tags for relative and absolute (iTRAQ) is widely used as a tool to quantify proteins expressed different cell types various cellular conditions. The precision iTRAQ quite high, accuracy dramatically decreases presence interference peptides that are coeluted coisolated with target peptide. Here, we developed "removal mixture MS/MS spectra...
Many animals depend on pheromone communication for successful mating. Sex in insects is usually released by females to attract males. In American cockroaches, the largest glomerulus (B-glomerulus) male antennal lobe (first-order olfactory center) processes major component of sex pheromone. Using intracellular recordings combined with fine neuroanatomical techniques, we provide evidence that female homolog B-glomerulus also acts as a pheromone–specific detector. Whereas ordinary glomeruli...
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. Here, we describe pSNAP, a method for proteome-wide NPCs by affinity enrichment puromycin- stable isotope-labeled polypeptides. pSNAP does require purification and/or chemical labeling, captures bona fide that characteristically exhibit...
Recent advancements in mass spectrometry-based proteomics have made it possible to conduct comprehensive protein analysis. In particular, the emergence of data-independent acquisition (DIA) method powered by machine learning has significantly improved identification efficiency. However, compared with conventional data-dependent (DDA) method, degree which peptides are uniquely identified DIA and DDA not been thoroughly examined. this study, we over 10,000 proteins using methods analyzed...
jPOST (https://jpostdb.org/) comprises jPOSTrepo (https://repository.jpostdb.org/) (over 2000 projects), a repository for proteome mass spectrometry data, the reanalysis of raw data based on standardised protocol using UniScore, and jPOSTdb (https://globe.jpostdb.org/) 600 datasets), database that integrates reanalysed data. The rescores MS/MS spectra new scale, to evaluate extent which spectral peaks correspond amino acid sequences identified by search engines. However, metadata registered...