This study aims to suggest the limits of silver nanoparticle (AgNP) uses for medicinal purpose and was performed explore effect various doses in rats. Four different AgNP (4, 10, 20, 40 mg/kg) were injected intravenously. For safety evaluation AgNP, body weight, organ coefficient, whole blood count, biochemistry panel assay liver function enzyme (AST, ALT, ALP, GGTP), comet assay, ROS, histological parameter performed; 10-12 week old animals randomly divided into groups six individuals each...

A quantum dot-based ratiometric pH sensor that responds to the range 6–8 was developed using FITC (fluorescein isothiocyanate) conjugated CdSe/CdZnS nanocrystals.

Fluorescence live imaging has become an essential methodology in modern cell biology. However, fluorescence requires excitation light, which can sometimes cause potential problems, such as autofluorescence, phototoxicity, and photobleaching. Furthermore, combined with recent optogenetic tools, the light illumination trigger their unintended activation. Because luminescence does not require it is a good candidate alternative modality to circumvent these problems. The application of imaging,...

Glutathione-coated PbS quantum dots with tunable emission were synthesized in the aqueous phase and used for non-invasive tissue imaging second near-infrared biological window.

T. Jin, K. Ichikawa and Koyama, J. Chem. Soc., Commun., 1992, 499 DOI: 10.1039/C39920000499

Gd3+-functionalized near-infrared emitting quantum dots were synthesized as a dual modal contrast agent for in vivofluorescence imaging and magnetic resonance imaging.

Glycans are expected to be one of the potential signal molecules for controlling drug targeting/delivery or long-term circulation biopharmaceuticals. However, effect carbohydrates artificially glycosylated derivatives on in vivo dynamic distribution profiles after intravenous injection model animals remains unclear due lack standardized methodology and a suitable platform. We report herein an efficient versatile method preparation multifunctional quantum dots (QDs) displaying common...

Single molecule fluorescence polarization techniques have been used for three-dimensional (3D) orientation measurements to observe the dynamic properties of single molecules. However, only few can simultaneously measure 3D and position. Furthermore, these often require complex equipment cumbersome analysis. We developed a microscopy system synthesized highly fluorescent, rod-like shaped quantum dots (Q rods), which linear polarizations, position fluorescent probe. The optics splits from...

This paper describes the synthesis and optical properties of PbS/CdS quantum dots for <italic>in vivo</italic> fluorescence imaging.

We report a one-step synthetic strategy for the preparation of recombinant protein (EGFP-Protein G)-coated PbS quantum dots dual (visible and second-NIR) fluorescence imaging breast tumors at cellular whole-body level.

Abstract Genetically encoded biosensors based on the principle of Förster resonance energy transfer comprise two major classes: fluorescence (FRET) and those bioluminescence (BRET). The FRET visualize signaling-molecule activity in cells or tissues with high resolution. Meanwhile, due to low background signal, BRET are primarily used drug screening. Here, we report a protocol transform intramolecular BRET-FRET hybrid called hyBRET biosensors. retain all properties prototype also work as...

Extending molecular imaging into the shortwave-infrared (SWIR, 900–1400 nm) region provides deep tissue visualization of biomolecules in living system resulting from low autofluorescence and scattering. Looking at Food Drug Administration-approved clinical trial near-infrared (NIR) probes, only indocyanine green (ICG) its analogues have been approved for biomedical applications. Excitation wavelength less than 800 nm limits these probes penetration noninvasive fluorescence imaging. Herein,...

Water-soluble CdSe/ZnS (core–shell) semiconductor quantum dots surface-modified with tetrahexyl ether derivatives of p-sulfonatocalix[4]arene were synthesized for the optical detection neurotransmitter acetylcholine.

A new method for the control of optical properties quantum dots (QDs) has been developed using calix[n]arene carboxylic acids (1−3) as surface coating agents QDs. The calixarene CdSe/ZnS QDs was easily performed in tetrahydrofuran at room temperature. Deprotonation carboxyl groups derivatives surrounding resulted highly fluorescent water-soluble emission peak calixarene-coated shifted to longer wavelengths depending on oligomer size derivative used coating. Although red shift decreases with...

Fluorescent probes that emit in the near-infrared (NIR, 700-1,300 nm) region are suitable as optical contrast agents for vivo fluorescence imaging because of low scattering and absorption NIR light tissues. Recently, quantum dots (QDs) have become a new class fluorescent materials can be used imaging. Compared with traditional organic dyes, QDs several unique advantages such size- composition-tunable emission, high brightness, narrow emission bands, large Stokes shifts, resistance to...

The early detection of HER2 (human epidermal growth factor receptor 2) status in breast cancer patients is very important for the effective implementation anti-HER2 antibody therapy. Recently, detections using conjugated quantum dots (QDs) have attracted much attention. QDs are a new class fluorescent materials that superior properties such as high brightness, resistance to photo-bleaching, and multi-colored emission by single-light source excitation. In this study, we synthesized three...

Abstract: Anti-HER2 antibody conjugated with quantum dots (anti-HER2ab-QDs) is a very recent fluorescent nanoprobe for HER2+ve breast cancer imaging. In this study we investigated in-vivo toxicity of anti-HER2ab CdSe/ZnS QDs in Wistar rats. For evaluation injected sample, body weight, organ coefficient, complete blood count (CBC), biochemistry panel assay (AST, ALT, ALP, and GGTP), comet assay, reactive oxygen species, histology, apoptosis were determined. rat (8–10 weeks old) randomly...

Near-infrared (NIR) fluorescent imaging is a powerful tool for the non-invasive visualization of inner structure living organisms. Recently, NIR fluorescence at 1000–1400 nm (second optical window) has been shown to offer better spatial resolution compared with conventional 700–900 (first window). Here we report lead sulfide (PbS) quantum dots (QDs) and their use in vivo cerebral venous thrombosis septic mice. Highly PbS QDs 1100 emission peak (QD1100) were prepared from acetate...

Recently, shortwave-infrared (SWIR) fluorescence imaging for the optical diagnostics of diseases has attracted much attention as a new noninvasive modality. For this application, development SWIR molecular probes with high biocompatibility is crucial. Although many types biocompatible fluorescent based on organic dyes have been reported, there are no SWIR-emitting that can be used detection specific biomolecules in vivo. To apply to biomedical fields, we developed dye π-conjugation extended...

Leuchtkugel: Der atomar monodisperse Pt5-Nanocluster hat eine niedrige Zytotoxizität und zeigt Fluoreszenzquantenausbeute von 18 %. Er kann als Fluoreszenzsonde für die zelluläre Bildgebung eingesetzt werden (siehe Schema; graue Kugel: Pt5-Nanocluster; PAMAM (G4-OH): Polyamidoamin-Dendrimer der vierten Generation; Skalierung 20 μm).

Bioluminescence resonance energy transfer coupled near-infrared quantum dots using glutathione-s-transferase (GST) tagged luciferase were synthesized as luminescent probes for in vivo imaging.

Incorporation of ICG into calix[4]arene micelles improves its aqueous stability and fluorescence brightness.