A5076516879- Glycosylation and Glycoproteins Research
- Sphingolipid Metabolism and Signaling
- Robotic Locomotion and Control
- Adaptive Control of Nonlinear Systems
- Balance, Gait, and Falls Prevention
- Iterative Learning Control Systems
- Robot Manipulation and Learning
- Teleoperation and Haptic Systems
- Muscle activation and electromyography studies
- Virus-based gene therapy research
- Caveolin-1 and cellular processes
- Guidance and Control Systems
- Robotic Mechanisms and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Prosthetics and Rehabilitation Robotics
- Cell Adhesion Molecules Research
- Galectins and Cancer Biology
- Lysosomal Storage Disorders Research
- CRISPR and Genetic Engineering
- Hydraulic and Pneumatic Systems
- Carbohydrate Chemistry and Synthesis
- Fuzzy Logic and Control Systems
- Erythrocyte Function and Pathophysiology
- Renal and related cancers
- Gait Recognition and Analysis
Azabu University
2016-2025
Chuo University
2025
East Japan Railway (Japan)
2020
Matrix Research (United States)
2019
Saiseikai Kumamoto Hospital
2018
Kyushu University
2018
Kyushu University Hospital
2018
Kyungpook National University
2011-2014
Hokkaido University
1995-2014
University of California, San Diego
2013
Sphingolipid signaling pathways have been implicated in many critical cellular events. Sphingosine-1-phosphate (SPP), a sphingolipid metabolite found high concentrations platelets and blood, stimulates members of the endothelial differentiation gene (Edg) family G protein-coupled receptors triggers diverse effects, including cell growth, survival, migration, morphogenesis. To determine vivo functions SPP/Edg pathway, we disrupted Edg1 mice. Edg1(-/-) mice exhibited embryonic hemorrhage...
NKT cells represent a distinct lineage of T that coexpress conserved alphabeta cell receptor (TCR) and natural killer (NK) receptors. Although the TCR is characteristically autoreactive to CD1d, lipid-presenting molecule, endogenous ligands for these have not been identified. We show lysosomal glycosphingolipid previously unknown function, isoglobotrihexosylceramide (iGb3), recognized both by mouse human cells. Impaired generation iGb3 in mice lacking beta-hexosaminidase b results severe...
Sphingosine-1-phosphate (S1P), an important sphingolipid metabolite, regulates diverse cellular processes, including cell survival, growth, and differentiation. Here we show that S1P signaling is critical for neural vascular development. Sphingosine kinase-null mice exhibited a deficiency of which severely disturbed neurogenesis, tube closure, angiogenesis caused embryonic lethality. A dramatic increase in apoptosis decrease mitosis were seen the developing nervous system. S1P(1)...
Gangliosides are sialic acid-containing glycosphingolipids that present on all mammalian plasma membranes where they participate in recognition and signaling activities. We have established mutant mice lack GM3 synthase (CMP-NeuAc:lactosylceramide α2,3-sialyltransferase; EC 2.4.99.-). These were unable to synthesize ganglioside, a simple widely distributed glycosphingolipid. The viable appeared without major abnormalities but showed heightened sensitivity insulin. A basis for the increased...
Glycosphingolipids (GSLs) are believed to be integral for the dynamics of many cell membrane events, including cellular interactions, signaling, and trafficking. We have investigated their roles in development differentiation by eliminating major synthesis pathway GSLs through targeted disruption Ugcg gene encoding glucosylceramide synthase. In absence GSL synthesis, embryogenesis proceeded well into gastrulation with primitive germ layers patterning embryo but was abruptly halted a...
Sphingosine-1-phosphate (S1P) elicits diverse cellular responses through a family of G-protein-coupled receptors. We have shown previously that genetic disruption the S1P1 receptor, most widely expressed family, results in embryonic lethality because its key role within endothelial cells regulating coverage blood vessels by vascular smooth muscle cells. To understand physiologic functions two other S1P receptors, we generated S1P2 and S1P3 null mice. Neither mice nor exhibited significant or...
Gangliosides, which are sialylated glycosphingolipids, the major class of glycoconjugates on neurons and carry majority sialic acid within central nervous system (CNS). To determine role ganglioside synthesis CNS, mice carrying null mutations in two critical ganglioside-specific glycosyltransferase genes, Siat9 (encoding GM3 synthase) Galgt1 GM2 synthase), were generated. These double-null unable to synthesize gangliosides ganglio-series CNS. Soon after weaning, viable developed a severe...
Aggregation of the receptor with high affinity for IgE (Fc epsilon RI) on surface mast cells and basophils stimulates phosphorylation protein tyrosines, a process in which p53/56lyn kinase has been implicated. We measured association between Fc RI kinase, using chemical crosslinking to stabilize their interaction. In rat basophilic leukemia cell line, 3-4%, at most 20%, appear be associated prior aggregation, even though there is an excess total lyn kinase. Aggregating causes three four...
Initial events of T-cell activation involve movement the receptor into lipid rafts. Gangliosides are major components While investigating in ganglioside-deficient mice, we observed that CD4 + and CD8 T cells required different ganglioside subsets for activation. Activation from GM3 synthase-null deficient GM3-derived gangliosides, is severely compromised, whereas normal. Conversely, GM2/GD2 expressing only GD3, normal, deficient. Supplementing with corresponding missing gangliosides restores...
The cytoplasmic peptide:N-glycanase (Ngly1 in mammals) is a de-N-glycosylating enzyme that highly conserved among eukaryotes. It was recently reported subjects harboring mutations the NGLY1 gene exhibited severe systemic symptoms (NGLY1-deficiency). While obviously has critical role mammals, its precise function remains unclear. In this study, we analyzed Ngly1-deficient mice and found they are embryonic lethal C57BL/6 background. Surprisingly, additional deletion of encoding...
Gaucher disease, the most common lysosomal storage is caused by a deficiency of glucocerebrosidase resulting in impairment glucosylceramide degradation. The hallmark disease presence cell, macrophage containing much stored found tissues, which believed to cause many clinical manifestations disease. We have developed adult mice carrying L444P point mutation (Gba) gene and exhibiting partial enzyme deficiency. mutant demonstrate multisystem inflammation, including evidence B cell...
Discusses force control for the tendon-sheath driving system typically used to actuate robotic finger joints. For a simple model, transmission characteristics were first formulated with apparent tendon-stiffness and equivalent backlash. An interesting aspect is that changes when tendon pulled or loosened, while itself keeps constant under absence of friction. This unexpected behavior confirmed by simulations as well experiments. The authors show direction-dependent eventually brings about...
Glycans are expected to be one of the potential signal molecules for controlling drug targeting/delivery or long-term circulation biopharmaceuticals. However, effect carbohydrates artificially glycosylated derivatives on in vivo dynamic distribution profiles after intravenous injection model animals remains unclear due lack standardized methodology and a suitable platform. We report herein an efficient versatile method preparation multifunctional quantum dots (QDs) displaying common...
The sodium-independent cystine-glutamate antiporter plays an important role in extracellular cystine uptake. It comprises the transmembrane protein, xCT and its chaperone, CD98. Because glutathione is only weakly cell membrane permeable, cellular uptake of precursor, cystine, known to be a key step synthesis. Moreover, it has been reported that expression affects progression tumors their resistance therapy. Sulfasalazine inhibitor increase oxidative stress, giving anti-tumor potential. Here,...
Polyphenols are abundant in vegetables and fruit. They have been shown to various antitumor, antioxidant, anti-inflammatory effects. Here, we extracted the lipid-soluble fraction of polyphenols from fermented sweet potato (Ipomoea batatas). These mainly contained caffeic acid derivatives with strong antioxidant ability, which hypothesized affect diseases for oxidative stress is a factor, such as cancer. We therefore investigated antitumor chemo-sensitizing effects on E0771 murine breast...
Gaucher disease, the most common lysosomal storage is caused by a deficiency of glucocerebrosidase resulting in impairment glucosylceramide degradation. The hallmark disease presence cell, macrophage containing much stored found tissues, which believed to cause many clinical manifestations disease. We have developed adult mice carrying L444P point mutation (Gba) gene and exhibiting partial enzyme deficiency. mutant demonstrate multisystem inflammation, including evidence B cell...
Mice require testicular glycosphingolipids (GSLs) for proper spermatogenesis. Mutant mice strains deficient in specific genes encoding biosynthetic enzymes of the GSL pathway including Galgt1 (encoding GM2 synthase) and Siat9 GM3 have been established lacking various overlapping subsets GSLs. Although male Galgt1-/- are infertile, Siat9-/- fertile. Interestingly, GSLs thought to be essential spermatogenesis not synthesized either these strains. Hence, cannot account different phenotypes. A...
Milk provides nutritional, immunological and developmental components for newborns. Whereas identification of such has been performed by targeting proteins free oligosaccharides, structural functional analyses the N-glycome milk glycoproteins are scarce. In this study, we investigated, first time, alterations bovine during early lactation (1 day, 1, 2, 3 4 weeks postpartum), characterizing more than 80 N-glycans. The glycomic profile colostrum on day 1 after calving differed substantially...
Varieties of transforming growth factor‐β (TGF‐β) antagonists have been developed to intervene with excessive TGF‐β signalling activity in cancer. Activin receptor‐like kinase5 (ALK5) inhibitors antagonize by blocking receptor‐activated Smad (R‐Smad) phosphorylation. Here we report the novel mechanisms how ALK5 exert a therapeutic effect on mouse B16 melanoma model. Oral treatment inhibitor, EW‐7197 (2.5 mg/kg daily) or representative LY‐2157299 (75 bid) suppressed progression enhanced...
Targeting ferroptosis, cell death caused by the iron-dependent accumulation of lipid peroxides, and disruption redox balance are promising strategies in cancer therapy owing to physiological characteristics cells. However, detection ferroptosis using vivo imaging remains challenging. We previously reported that maps showing reduction power per unit time implanted tumor tissues via non-invasive a novel, compact, portable electron paramagnetic resonance (EPRI) device could be compared with...
Although various glycoforms appear to participate independently in multiple molecular interactions cellular adhesion that contribute embryogenesis and organogenesis, a full portrait of the glycome diversity effect structural variations on individual cell stages proliferation differentiation remain unclear. Here we describe novel concept for characterization dynamic glycoform alteration during by means "glycoblotting-based glycomics," only method allowing rapid quantitative glycan analysis....