A5062716929- Toxoplasma gondii Research Studies
- Parasitic Infections and Diagnostics
- Crop Yield and Soil Fertility
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Microbial Metabolic Engineering and Bioproduction
- Amoebic Infections and Treatments
- Research on Leishmaniasis Studies
- Gene Regulatory Network Analysis
- Soybean genetics and cultivation
- Diagnosis and treatment of tuberculosis
- Trypanosoma species research and implications
- Genomics and Phylogenetic Studies
- Insect symbiosis and bacterial influences
- Environmental Toxicology and Ecotoxicology
- Bioinformatics and Genomic Networks
- Bacteriophages and microbial interactions
- Parasites and Host Interactions
- Marine Biology and Ecology Research
- Ruminant Nutrition and Digestive Physiology
- Malaria Research and Control
- Pancreatic function and diabetes
- Atmospheric and Environmental Gas Dynamics
- Weed Control and Herbicide Applications
- Agricultural Economics and Policy
University of Nebraska at Omaha
2016-2025
Virginia Cooperative Extension
2008-2020
Virginia Tech
2000-2020
University of Nebraska Medical Center
2012-2018
University of Pennsylvania
2008-2010
Washington University in St. Louis
2003-2009
Wellcome Sanger Institute
2005
Lawrence Livermore National Laboratory
1979-1982
Los Angeles City College
1969
Let Me Out Apicomplexan parasites like Plasmodium falciparum , which causes malaria, and Toxoplasma gondii cause toxoplasmosis, replicate inside animal host cells. In order for infections to spread successfully within the from cell cell, daughter after replication need be able escape their incubator cell. course of studies intended elucidate functions proteases during parasite infection, Chandramohanadas et al. (p. 794 published online 2 April) noted that calpain is only protease present at...
Toxoplasma gondii is a prevalent obligate intracellular parasite which chronically infects more than third of the world's population. Key to prevalence its ability form chronic and nonimmunogenic bradyzoite cysts, typically in brain muscle cells infected mammals, including humans. While acute clinical infection involves neurological and/or ocular damage, has been recently linked behavioral changes. Establishment maintenance balance between host immunity evasion immune response. Here, we...
Summary Entamoeba histolytica is a protozoan intestinal parasite that causes amoebic colitis and liver abscess. To identify virulence factors of E. , we first defined the phenotypes two strains, HM‐1:IMSS, prototype virulent strain, Rahman, strain was reportedly less than HM‐1:IMSS. We found compared with Rahman has defect in erythrophagocytosis ability to cause human colonic xenografts. used differential in‐gel 2D electrophoresis compare proteome identified six proteins were differentially...
Background: There are numerous botanical extracts and fractions that have been shown to a diverse range of bioactivities. Many nutraceutical products food supplements contain phytochemicals - often multiple numbers them as constituents single product. is paucity data on the net bioactivities these multi-component products. This paper reports effects four such expression six genes critical maintenance good health. Objectives: We investigated several number derived from plant species well...
The rise of bacterial infections is a global health issue that calls for the development and availability additional antimicrobial agents. Known its in vitro effects on Gram-positive organisms, drug-like small molecule marinopyrrole A was re-examined potential broader efficacy against wider array microbes. We uncovered selective an important subset Gram-negative bacteria from three genera: Neisseria, Moraxella, Campylobacter. This susceptibility correlated with absence canonical LPS these...
Abstract Background Microarrays are invaluable tools for genome interrogation, SNP detection, and expression analysis, among other applications. Such broad capabilities would be of value to many pathogen research communities, although the development use genome-scale microarrays is often a costly undertaking. Therefore, effective methods reducing unnecessary probes while maintaining or expanding functionality relevant investigators. Results Taking advantage available sequences annotation...
The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is a vector for the causative agents of Huanglongbing, which threatens production worldwide. This study reports and discusses first D. transcriptomes, encompassing three main life stages citri, egg, nymph adult. transcriptomes were annotated using Gene Ontology (GO) insecticide-related genes within each stage identified to aid development future insecticides. Transcriptome assemblies other sequence data are available...
Due to its role in maintaining the proper functioning of cell, pentose phosphate pathway (PPP) is one most important metabolic pathways. It responsible for regulating concentration simple sugars and provides precursors synthesis amino acids nucleotides. In addition, it plays a critical an adequate level NADPH, which necessary cell fight oxidative stress. These reasons prompted authors develop computational model, based on queueing theory, capable simulating changes PPP metabolites'...
The availability of two genetically very similar cell lines (A and B) derived from the laboratory isolate Entamoeba histolytica HM-1:IMSS, which differ in their virulence properties, provides a powerful tool for identifying pathogenicity factors causative agent human amoebiasis. Cell line A is incapable inducing liver abscesses gerbils, whereas interaction with B leads to considerable abscess formation. Phenotypic characterization both revealed that trophozoites pathogenic have larger size,...
The protozoan intestinal parasite Entamoeba histolytica infects millions of people worldwide and is capable causing amebic dysentery liver abscess. closely related species dispar colonizes many more individuals, but this organism does not induce disease. To identify molecular differences between these two organisms that may account for their differential ability to cause disease in humans, we used two-dimensional gel-based (DIGE) proteomic analysis compare whole cell lysates E. dispar. We...
As an intracellular protozoan parasite, Toxoplasma gondii is likely to exploit proteases for host cell invasion, acquisition of nutrients, avoidance protective responses, escape from the parasitophorous vacuole, differentiation, and other activities. T. serine protease inhibitor 1 (TgPI1) most abundantly expressed in parasite tachyzoites. We show here that alternative splicing produces two TgPI1 isoforms, both which are secreted via dense granules into vacuole shortly after become...
The objectives of the present study were to determine 1) if temporal variability influenced toxicity Elkhorn River, Nebraska, USA, water, and 2) toxic effect was consistent between 2 sentinel organisms, fathead minnow (Pimephales promelas) northern leopard frog (Rana pipiens). During spring 2012, atrazine indicator strips used document occurrence agrichemical pulses in River. Polar organic chemical integrative samplers (POCIS) deployed for 14 d during both a pulse postpulse period as...
Maintaining winter wheat (Triticum aestivum L.) productivity with more efficient nitrogen (N) management will enable growers to increase profitability and reduce the negative environmental impacts associated loss. Wheat breeders would therefore benefit greatly from identification application of genetic markers use efficiency (NUE). To investigate genetics underlying N response, two bi-parental mapping populations were developed grown in four site-seasons under low high rates. The derived a...
Semisynthetic artemisinins and other bioactive peroxides are best known for their powerful antimalarial activities, they also show substantial activity against schistosomes—another hemoglobin-degrading pathogen. Building on this discovery, we now describe the initial structure–activity relationship (SAR) of antischistosomal ozonide carboxylic acids OZ418 (2) OZ165 (3). Irrespective lipophilicity, these weak have relatively low aqueous solubilities high protein binding values. Ozonides with...