Astilbin (ASN) is a flavonoid compound isolated from the rhizome of Smilax china L. (Smilacaceae). It has many bioactivities, such as selective immunosuppression, antioxidant, anti-hepatic injury, etc., and widely used in traditional Chinese medical treatments. The interaction ASN with bovine serum albumin (BSA) was studied physiological buffer (pH = 7.40) using multi-spectroscopic techniques combination molecular docking methods. UV-vis absorption measurements proved that ASN-BSA complex...

Abstract The advanced glycation end products (AGEs) are the compounds produced by non‐enzymatic reaction of proteins and sugars, which can induce generation free radicals expression inflammatory factors, thereby playing an important role in vascular dysfunction diabetes. To investigate effects caffeic acid (CA) on formed glucose protein, various spectroscopic techniques molecular docking methods were carried out. Furthermore, protective CA human umbilical vein endothelial cells (HUVECs)...

Scoparone is a major biological active substance derived from the traditional Chinese herbal medicine called Artemisia capillaris. It has been confirmed that scoparone anti-inflammatory, anti-tumor, hepatoprotective and antioxidant effects. However, binding interaction of with bovine serum albumin (BSA) still remains unknown. Therefore, present study was conducted to clarify BSA under simulated physiological conditions (pH = 7.4) by utilizing spectroscopic molecular docking methods. The...

Cervical cancer is one of the cancers commonly found in female reproductive system and associated with obesity. However, exact connection mechanisms remain unclear. Screening key therapeutic targets natural products good anti-tumor activity has become a crucial strategy for therapy. Cryptotanshinone anti-inflammatory anti-cancer properties. Key related low-toxicity active ingredients were identified as components treatment strategies. Therefore, network pharmacology cellular biology...

Introduction: Breast cancer is one of the most commonly diagnosed cancers in women, with a high mortality rate. Objective: In present study, we evaluated anticancer effect nobiletin, flavone glycoside, on breast cell line MCF-7. Result: Cell viability and proliferation decreased morphology changed from diamond to round after being treated nobiletin. Nobiletin induced apoptosis MCF-7 cells via regulating protein expression Bax, Bcl-2, cleaved caspase-3, p53. The Bcl-2 decreased, while Bax p53...

Human breast cancer is a malignant type of with high prevalence. In the present study, anticancer effects alantolactone, sesquiterpene lactone, on human cell line MCF‑7 were investigated in vitro. The morphology changed from diamond to round subsequent treatment and viability reduced significantly compared that control cells. Alantolactone induced apoptosis cells by regulating protein expression levels B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein, p53, caspase‑3 caspase‑12, which...

Abstract Maillard reaction occurs between the carbonyl group of reducing sugars and free amino groups protein, which eventually results in formation accumulation advanced glycation end products (AGEs) irreversibly. Excessive production AGEs is associated with many diseases, such as Alzheimer disease, neuropathy, retinopathy, nephropathy. In this study, effects eriodictyol naringenin on inhibition were studied bovine serum albumin (BSA)–methylglyoxal (MGO) model by spectroscopic techniques...

Alantolactone is a sesquiterpene lactone extracted from Inula helenium L. plants possessing many biological activities, including anti-inflammatory, antiproliferation, and antimicrobial. The inhibitory effects the underlying mechanisms of alantolactone on lung cancer cells NCI-H1299 Anip973 were investigated in this study. results showed that could decrease cell viability induce apoptosis Anip973. After treated with alantolactone, expression Bcl-2 decreased, while Bax increased, MMP-9,...

In the aftermath of COVID pandemic, we have been promoting online learning as a new tool. This study investigated design optimal structure self-assisting coursework for laboratory courses that can assist students to better prepare hands-on experiments. Undergraduate from Liaoning University, who took “Biochemistry Experiments” class, were asked evaluate self-learning segment offered on Rain Classroom, an platform was provided before each in-person lab session preparation. Survey questions...

Eriocitrin is a flavanone glycoside, which exists in lemon or lime citrus fruits. It possesses antioxidant, anticancer, and anti-allergy activities. In order to investigate the pharmacokinetics pharmacological mechanisms of eriocitrin vivo, interaction between bovine serum albumin (BSA) was studied under simulated physiological conditions by multispectroscopic molecular docking methods. The results well indicated that BSA formed new eriocitrin-BSA complex because intermolecular interactions,...

Gastric cancer is a malignant tumor with high incidence rate and mortality rate.In this study, we investigated the anti-cancer effect of alantolactone, sesquiterpene lactone, on gastric cell lines BGC-823 SGC-7901.BGC-823 SGC-7901 cells were treated different concentrations Hoechst 33258 staining, flow cytometry, wound healing assay, invasion colony forming quantative polymerase chain reaction, western blot analysis used to evaluate anticancer activity alantolactone cancer.Alantolactone...

Abstract Chronic gastritis (CG) and osteoporosis (OP) are common occult diseases in the elderly relationship of these two have been increasingly exposed. We aimed to explore clinical characteristics shared mechanisms CG patients combined with OP. In cross-sectional study, all participants were selected from BEYOND study. The included classified into groups, namely OP group non-OP group. Univariable multivariable logistic regression methods used evaluate influencing factors. Furthermore,...

Linear and two-dimensional infrared (IR) spectroscopy of site-specific probe molecules provides an opportunity to gain a molecular-level understanding the local hydrogen-bonding network, conformational dynamics, long-range electrostatic interactions in condensed-phase biological systems. A challenge computation is determine time-dependent vibrational frequencies that incorporate explicitly both nuclear quantum effects motions electronic structural representation potential energy surface. In...

Molecular dynamics simulation of human heart lactate dehydrogenase (LDH) has been carried out to determine the linear and two-dimensional Fourier transform infrared (2D-FTIR) spectra for carbonyl stretch vibration pyruvate in tetrameric enzyme, using quantum vibrational perturbation theory. The computed line-shapes individual subunits are inhomogeneously broadened span entire absorption range full indicating similar conformation heterogeneity four active sites LDH. However, each subunit...

Black cutworm (BCW), Agrotis ipsilon (Hufnagel), is an occasional pest of maize that can cause considerable economic loss and injury to corn seedlings. This research mainly assessed the susceptibility BCW neonates 11 Bt toxins (Cry1Ab, Cry1Ac, Cry1Ah, Cry1F, Cry1Ie, Cry1B, Cry2Aa, Vip3_ch1, Vip3_ch4, Vip3Ca2, Vip3Aa19) by exposing artificial diet containing evaluated efficacy three transgenic events (C008, C009, C010) expressing Vip3Aa19 toxin against BCW. The toxin-diet bioassay data...

Abstract Oxidative damage in cells induced by reactive oxygen species (ROS) is a main factor diabetes mellitus diseases progression. The composition of anthocyanins from Padus racemosa (APR) and the protective effects APR on H 2 O ‐induced rat insulinoma (INS‐1) streptozocin (STZ)‐induced mice were investigated this study. components cyanidin‐cyanidin glucosyl‐rutinoside, xylosyl‐rutinoside, cyanidin‐xylosyl‐glucoside cyanidin‐rutinoside, which determined liquid chromatography‐mass...

Objective To confirm the role of long noncoding RNA differentiation antagonizing non‐protein coding (DANCR) in chondrocyte inflammatory injury osteoarthritis (OA) vitro , as well its molecular mechanism. Methods Human primary chondrocytes were treated with lipopolysaccharide (LPS) to construct a human OA cell model. Gene expression was detected using real‐time quantitative polymerase chain reaction. Cell evaluated by Counting Kit‐8 assay, flow cytometry, and enzyme‐linked immunosorbent...