A5054847913- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Nuclear Structure and Function
- Aortic Disease and Treatment Approaches
- DNA Repair Mechanisms
- Conservation Techniques and Studies
- Chromosomal and Genetic Variations
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Biosensors and Analytical Detection
- Cardiac Valve Diseases and Treatments
- Connective tissue disorders research
- Genomics and Chromatin Dynamics
- Adipose Tissue and Metabolism
- Genetics, Aging, and Longevity in Model Organisms
- Aortic aneurysm repair treatments
- Autophagy in Disease and Therapy
- Cellular Mechanics and Interactions
- Phagocytosis and Immune Regulation
- Adipokines, Inflammation, and Metabolic Diseases
- Gut microbiota and health
- Sirtuins and Resveratrol in Medicine
- Cardiac Structural Anomalies and Repair
- Cell Image Analysis Techniques
- Cultural Heritage Materials Analysis
Fifth Affiliated Hospital of Sun Yat-sen University
2025
University of California, Berkeley
2020-2024
Lawrence Berkeley National Laboratory
2024
QB3
2020-2024
The First Affiliated Hospital, Sun Yat-sen University
2018-2024
Sun Yat-sen University
2018-2024
Columbia University
2015-2024
University of California, Los Angeles
2023-2024
Liaoning University
2023-2024
Howard Hughes Medical Institute
2021-2023
Abstract Abnormal glucose and lipid metabolism in COVID-19 patients were recently reported with unclear mechanism. In this study, we retrospectively investigated a cohort of without pre-existing metabolic-related diseases, found new-onset insulin resistance, hyperglycemia, decreased HDL-C these patients. Mechanistically, SARS-CoV-2 infection increased the expression RE1-silencing transcription factor (REST), which modulated secreted metabolic factors including myeloperoxidase, apelin,...
Background Although glycoursodeoxycholic acid (GUDCA) has been associated with the improvement of metabolic disorders, its effect on atherosclerosis remains elusive. This study aimed to investigate role GUDCA in development and potential mechanisms. Methods Results Human THP‐1 macrophages were used oxidized low‐density lipoprotein–induced foam cell formation vitro. We found that downregulated scavenger receptor A1 mRNA expression, reduced lipoprotein uptake, inhibited macrophage formation....
Meiotic chromosome segregation relies on synapsis and crossover (CO) recombination between homologous chromosomes. These processes require multiple steps that are coordinated by the meiotic cell cycle monitored surveillance mechanisms. In diverse species, failures in can trigger a delay and/or lead to apoptosis. How this key step ‘homolog engagement’ is sensed transduced cells unknown. Here we report C. elegans , recruitment of Polo-like kinase PLK-2 synaptonemal complex triggers...
Interactions between chromosomes and LINC (linker of nucleoskeleton cytoskeleton) complexes in the nuclear envelope (NE) promote homolog pairing synapsis during meiosis. By tethering to cytoskeletal motors, these connections lead processive chromosome movements along NE. This activity is usually mediated by telomeres, but nematode Caenorhabditis elegans, special regions called "pairing centers" (PCs) have acquired this meiotic function. Here, we identify a previously uncharacterized...
Oogenesis involves transduction of mechanical forces from the cytoskeleton to nuclear envelope (NE). In
Centromeres of higher eukaryotes are epigenetically defined by centromere protein A (CENP-A), a centromere-specific histone H3 variant. The incorporation new CENP-A into centromeres to maintain the epigenetic marker after genome replication in S phase occurs G1 phase; however, how is loaded and stabilized remains poorly understood. Here, we identify formin mDia2 as essential for stable replenishment at centromeres. Quantitative imaging, pulse-chase analysis, high-resolution ratiometric...
Centromeres are specialized chromosomal regions epigenetically defined by the histone H3 variant centromere protein A (CENP-A). CENP-A needs to be replenished in every cell cycle, but how new is stably incorporated into centromeric chromatin remains unclear. We have discovered that a cytoskeletal protein, diaphanous formin mDia2, essential for stable incorporation of proteins nucleosomes. Here we report mDia2-mediated formation dynamic and short nuclear actin filaments G1 nucleus required...
In the aftermath of COVID pandemic, we have been promoting online learning as a new tool. This study investigated design optimal structure self-assisting coursework for laboratory courses that can assist students to better prepare hands-on experiments. Undergraduate from Liaoning University, who took “Biochemistry Experiments” class, were asked evaluate self-learning segment offered on Rain Classroom, an platform was provided before each in-person lab session preparation. Survey questions...
Sexual reproduction relies on robust quality control during meiosis. Assembly of the synaptonemal complex between homologous chromosomes (synapsis) regulates meiotic recombination and is crucial for accurate chromosome segregation in most eukaryotes. Synapsis defects can trigger cell cycle delays and, some cases, apoptosis. We developed deployed a chemically induced proximity system to identify key elements this pathway Caenorhabditis elegans . Persistence polo-like kinase PLK-2 at pairing...
Dynein light chains are accessory subunits of the cytoplasmic dynein complex, a minus-end directed microtubule motor. Here, we demonstrate that chain Tctex-1 associates with unattached kinetochores and is essential for accurate chromosome segregation. knockdown in cells does not affect localization function at kinetochore, but produces prolonged mitotic arrest few misaligned chromosomes, which subsequently missegregated during anaphase. This independent Tctex-1's association dynein. The...
Obesity is one of the most prevalent chronic metabolic diseases, and induction apoptosis in preadipocytes adipocytes a potential strategy to treat obesity. Celastrol represents robust anti-obesity phytochemicals so far, yet its direct binding target remains elusive. Here, we determined that celastrol could induce via mitochondrial mediated pathway. Further study clarified inhibited fusion autophagosome lysosome prohibit autophagy, leading cell apoptosis. By conducting virtual screening...
Head and neck squamous cell carcinomas are the sixth most common malignant tumors worldwide. Tongue carcinoma is a tumor of this type, it associated with poor prognosis, high rate recurrence low survival rate. Plumbagin derived from Plumbago zeylanica L, several studies report that plumbagin could inhibit cell, metastasis, induce apoptosis in various cancer cells. Patient-derived xenograft (PDX) model can maintain heterogeneity microenvironment human tumors, powerful research tool for...
Abstract Efficient removal of mold stains becomes an important research topic for paper conservation. In this study, a cleaning scheme based on the combination bioenzymes and biosurfactants was explored. Morphological molecular biology identifications were first jointly applied to identify dominant strains sampled from five ancient books that are stored in same environment. Cellulolytic experiments then conducted evaluate cellulose degradation ability according cellulolytic digestive index....
During mitosis, Aurora B kinase is required for forming proper bi-oriented kinetochore-microtubule attachments. Current models suggest that tension exerted between a pair of sister-kinetochores (inter-kinetochore stretch) produces spatial separation from kinetochore-associated microtubule binding substrates, such as the Knl1-Mis12-Ndc80 (KMN) network, resulting in decrease phosphorylation and, thus, an increase affinity microtubules. Using Single-Molecule High-Resolution Colocalization...
Critical limb ischemia (CLI) is a severe form of peripheral artery diseases (PAD) and seriously endangers the health people. Therapeutic angiogenesis represents an important treatment strategy for CLI; various methods have been applied to enhance collateral circulation. However, current development drug therapy promote limited. Resveratrol (RSV), polyphenol compound extracted from plants, has properties such as anti-oxidative, anti-inflammatory anti-cancer effects. Whether RSV exerts...
Previous studies examining whether autophagy has a protective or deleterious role during ischemia/reperfusion (I/R) injury have reported varying in different organs and remains matter of debate. The aim the current study was to explore hindlimb I/R murine model. An increase apoptosis observed vitro, C2C12 myoblast cells, following hypoxia/reoxygenation (H/R), while downregulation autophagic flux induced by chloroquine as compared with vehicle group under hypoxia H/R conditions. In vivo, an...
Aortic intramural hematoma (IMH) is a subset of acute aortic syndrome, and its prognosis may differ between races. This study aimed to the Chinese type B IMH patients find out risk factors. A total 71 with or without penetrating atherosclerosis ulcer (PAU) administrated in our center September 2013 October 2017 were retrospectively studied. Both clinical imaging data collected analyzed. The primary end point was aorta-related death, secondary progression, which defined as enlargement aorta,...