A5101468125- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Biochemical Analysis and Sensing Techniques
- Ion channel regulation and function
- Regulation of Appetite and Obesity
- Neuroendocrine regulation and behavior
- Nitric Oxide and Endothelin Effects
- Pharmacogenetics and Drug Metabolism
- Inflammatory mediators and NSAID effects
- Pharmacological Effects and Assays
- Neurobiology and Insect Physiology Research
- Gastrointestinal motility and disorders
- Pain Mechanisms and Treatments
- Diet and metabolism studies
- Drug Transport and Resistance Mechanisms
- Neuroscience and Neuropharmacology Research
- Zebrafish Biomedical Research Applications
- Physiological and biochemical adaptations
- Adipose Tissue and Metabolism
- Ion Channels and Receptors
- Mitochondrial Function and Pathology
- Neurotransmitter Receptor Influence on Behavior
- Muscle metabolism and nutrition
- Phenothiazines and Benzothiazines Synthesis and Activities
- Estrogen and related hormone effects
Rakuno Gakuen University
2015-2025
GTx (United States)
2006
Food & Nutrition
2005
Nutrition Sciences (Belgium)
2005
KU Leuven
2005
Kyowa Kirin (Japan)
1993-2000
Georgetown University
1997-1999
Georgetown University Medical Center
1999
University of Virginia
1989-1994
University School
1994
The Concise Guide to PHARMACOLOGY 2021/22 is the fifth in this series of biennial publications. provides concise overviews, mostly tabular format, key properties nearly 1900 human drug targets with an emphasis on selective pharmacology (where available), plus links open access knowledgebase source and their ligands ( www.guidetopharmacology.org ), which more detailed views target ligand properties. Although constitutes over 500 pages, material presented substantially reduced compared...
Abstract alpha-Adrenergic (phenylephrine) and muscarinic (carbachol) agonists inositol 1,4,5-trisphosphate caused calcium release contractions in smooth muscle strips permeabilized with Staphylococcus aureus alpha-toxin. The responses to phenylephrine carbachol required or were potentiated by added GTP could be inhibited GDP beta S. also increased the contractile response of portal vein cytoplasmic Ca2+. We conclude that while G-protein-coupled phosphatidylinositol cascade, through...
inositol 1,4,5-trisphosphate; GTPrS, guanosine 5'-3-0-(thio) The abbreviations used are: SR, sarcoplasmic reticulum; InsP3, triphosphate; PIPES, piperazine-N,N'-bis(2-ethanesulfonic acid); HEPES, N-2-hydroxyethylpiperazine-N"2-ethanesulfonic acid; FCCP, carbonyl cyanidep-trifluoromethoxyphenylhydrazone; EGTA, [ethylene his(oxyethylenenitri1o)tetraacetic MPA, main pulmonary artery; MOPS, 3-(N-morpholino
The Concise Guide to PHARMACOLOGY 2023/24 is the sixth in this series of biennial publications. provides concise overviews, mostly tabular format, key properties approximately 1800 drug targets, and about 6000 interactions with 3900 ligands. There an emphasis on selective pharmacology (where available), plus links open access knowledgebase source targets their ligands ( https://www.guidetopharmacology.org ), which more detailed views target ligand properties. Although constitutes almost 500...
The mechanism of G protein-mediated sensitization the contractile apparatus smooth muscle to Ca2+ was studied in receptor-coupled alpha-toxin-permeabilized rabbit portal vein muscle. To test hypothesis that is due inhibition myosin light-chain (MLC) phosphatase activity, we measured effect guanosine 5'-[gamma-thio]triphosphate and phenylephrine on rate MLC dephosphorylation muscles preactivated with incubated Ca(2+)- ATP-free solution containing...
The amplitude of interrupted contractions evoked by noradrenaline or caffeine in Ca2+‐free, high‐K+ solutions containing EGTA La3+ was determined small (40‐60 micron thick) bundles guinea‐pig portal anterior mesenteric vein. Interrupted were produced removing the stimulating agent as soon tension record reached its peak. distribution intracellular Ca2+ determined, with electron probe X‐ray microanalysis, cryosections preparations frozen relaxed state and at peak noradrenaline‐induced...
Mechanisms of Ca2+ sensitization both myosin light chain (MLC) phosphorylation and force development by protein kinase C (PKC) were studied in permeabilized tonic smooth muscle obtained from the rabbit femoral artery. For comparison, sensitizing effect guanosine 5'-O-(gamma-thiotriphosphate) (GTP gamma S) was examined, which had been previously shown to inhibit MLC phosphatase phasic vascular muscle. We now report that PKC activators (phorbol esters, short synthetic diacylglycerols a...
CPI‐17 has recently been identified as a novel protein in vascular smooth muscle. In vitro , its phosphorylation and thiophosphorylation by kinase C (PKC) specifically inhibits the type 1 class of phosphatases, including myosin light chain (MLC) phosphatase. Both phosphorylated states dose‐dependently potentiated submaximal contractions at constant [Ca 2+ ] β‐escin‐permeabilized Triton X‐100‐demembranated arterial muscle, but produced no effect intact less intensely permeabilized (α‐toxin)...
1. Triton X-100-demembranated smooth muscle loses Ca2+-sensitizing responsiveness to protein kinase C (PKC) activators while intact and alpha-toxin-permeabilized muscles remain responsive. We attempted reconstitute the contractile Ca2+ sensitization by PKC in demembranated preparations. 2. Western blot analyses showed that content of alpha-isoform (PKCalpha) was markedly reduced muscle-specific phosphatase-1 inhibitor CPI-17 not detectable, amount calponin actin still remained similar those...
17 beta‐Oestradiol (E2) at 0.1‐10 microM directly inhibited various tonic and phasic smooth muscle contractions. The mechanism(s) of oestrogen‐induced inhibition contraction was studied using intact permeabilized strips isolated single cells muscle. 2. In endothelium‐denuded vascular muscle, E2 attenuated high K(+)‐induced force development myosin light chain phosphorylation, produced rapid reversible relaxation. There were no significant differences in these inhibitory effects between...
<b>Background and aims:</b> The gastroprokinetic activities of ghrelin, the natural ligand growth hormone secretagogue receptor (GHS-R), prompted us to compare effect ghrelin with that synthetic peptide (growth releasing 6 (GHRP-6)) non-peptide (capromorelin) GHS-R agonists both in vivo vitro. <b>Methods:</b> In vivo, dose dependent effects (1–150 nmol/kg) GHRP-6, capromorelin on gastric emptying were measured by <sup>14</sup>C octanoic breath test which was adapted for use mice. atropine,...
Zebrafish are used widely in biomedical, toxicological, and developmental research, but information on their xenobiotic metabolism is limited. Here, we characterized the expression of 14 cytochrome P450 (CYP) subtypes whole embryos larvae zebrafish (4 to 144 h post-fertilization (hpf)) metabolic activities several representative human CYP substrates. The CYPs showed various changes patterns during development. Many transcripts abruptly increased at about 96 hpf, when hepatic outgrowth...
The potential functional roles of M<sub>3</sub> muscarinic receptors in mouse atria were examined by pharmacological and molecular biological techniques, using wild-type mice, M<sub>2</sub> or receptor single knockout (M<sub>2</sub>KO, M<sub>3</sub>KO), double mice (M<sub>2</sub>/M<sub>3</sub>KO). Real-time quantitative reverse transcriptase-polymerase chain reaction analysis showed that the mRNA was expressed predominantly but M<sub>1</sub>, M<sub>3</sub>, M<sub>4</sub>, M<sub>5</sub>...