A5101606443- Traditional Chinese Medicine Analysis
- Phytochemistry and Biological Activities
- Metabolomics and Mass Spectrometry Studies
- Pharmacological Effects of Natural Compounds
- Natural product bioactivities and synthesis
- Tryptophan and brain disorders
- Bioactive Compounds and Antitumor Agents
- Phytochemical Studies and Bioactivities
- Phytochemistry and Bioactive Compounds
- Gut microbiota and health
- Flavonoids in Medical Research
- Chromatography in Natural Products
- Plant-based Medicinal Research
- Sphingolipid Metabolism and Signaling
- Alzheimer's disease research and treatments
- Medicinal Plants and Bioactive Compounds
- Ginseng Biological Effects and Applications
- Autophagy in Disease and Therapy
- Phytochemical and Pharmacological Studies
- Liver Disease Diagnosis and Treatment
- Ion Transport and Channel Regulation
- Nanoplatforms for cancer theranostics
- Cholesterol and Lipid Metabolism
- Inflammatory mediators and NSAID effects
- Medicinal Plants and Neuroprotection
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2025
Guangxi University
2023
China Academy of Chinese Medical Sciences
2006-2022
Institute of Chinese Materia Medica
2017-2020
Tokyo University of Pharmacy and Life Sciences
2018
Second Affiliated Hospital of Harbin Medical University
2016
Harbin Medical University
2016
Abstract Background In recent years, excellent results have suggested an association between the “brain-gut” axis and Alzheimer’s disease (AD) progression, yet role of in AD pathogenesis still remains obscure. Herein, we provided a potential link central peripheral neuroinflammatory disorders progression. Methods The Morris water maze (MWM) test, immunohistochemistry, ELISA, ProcartaPlex Multiplex immunoassay, multiple LC-MS/MS methods, V3-V4 regions 16S rRNA genes were applied to explore...
Arenobufagin (ArBu) is a natural anticancer drug with good anti-tumor effects, but its clinical applications and development potential are limited due to toxicity. The purpose of this study reduce the toxic side effects ArBu improve efficacy tumor treatment by incorporating it into poly(ethylene glycol)-b-poly (lactide) co-polymer (PEG-PLA). ArBu@PEG-PLA micelles were prepared thin film hydration method. optimized characterized size, stability, loading, encapsulation rate, release....
Abstract Lepidium meyenii (Maca), originated from Peru, has been cultivated widely in China as a popular health care food. However, the chemical and effective studies of Maca were less in-depth, which restricted its application seriously. To ensure quality Maca, feasible accurate strategy was established. One hundred sixty compounds including 30 reference standards identified 6 fractions methanol extract by UHPLC-ESI-Orbitrap MS. Among them, 15 representative active simultaneously determined...
Bioenergetic therapy based on tumor glucose metabolism is emerging as a promising therapeutic modality. To overcome the poor bioavailability and toxicity of arenobufagin (ArBu), MOF-derived intelligent nanosystem, ZIAMH, was designed to facilitate energy deprivation by simultaneous interventions glycolysis, OXPHOS TCA cycle. Herein, zeolitic imidazolate framework-8 loaded with ArBu indocyanine green, encapsulated within metal-phenolic networks for chemodynamic hyaluronic acid modification...
This study aims to comprehensively analyze the material basis of toad visceral oil(hereafter referred as oil), and explore pharmacological effect oil on atopic dermatitis(AD). Ultra-high performance liquid chromatography-linear ion trap/orbitrap high-resolution mass spectrometry(UHPLC-LTQ-Orbitrap-MS) gas chromatography-mass spectrometry(GC-MS) were employed identify chemical components in oil. The animal model AD was prepared by hapten stimulation method. modeled animals respectively...
An approach was established to analyze the chemical profiling of Xiao-Cheng-Qi Decoction (XCQD) using liquid chromatography coupled with electrospray ionization tandem mass spectrometry. XCQD consisted three herbal medicines (Rhubarb, Fructus Aurantii Immaturus and Cortex Magnoliae Officinalis). The traditional water extractive method applied in sample preparation, which identical clinical use. characteristic fragmentation pathways 17 reference compounds were comprehensively studied,...
Cinobufacin injection, also known as huachansu, is a preparation form of Cinobufacini made from extract liquid. Despite that injection shown to shrink liver and gastric tumors, improving patient survival life quality, the effective components in remain elusive. In this study, we aim screen antitumor elucidate most for treatment cancers.High performance liquid chromatography (HPLC) LC-MS/MS analysis were used separate determine injection. Inhibition rates various on cancer cells determined...
Toad skin and toad venom, as two kinds of Chinese medicine, are prepared from Bufo bufo gargarizans Cantor melanostictus Schneider. However, they display distinct properties in traditional application, the hydrophilic ingredients possible distinguishing chemicals between them. In this work, 36 22 components were characterized respectively, by UHPLC-HR-MS/MS, including amino acids, nucleosides, polypeptides, indolealkylamines (IAAs). Among them, 15 compounds unambiguously confirmed comparison...
Cinobufacini injection, prepared from the skin of B ufo bufo gargarizans C antor, has presented its significant effects on treatment hepatitis and various cancers in clinic. However, as an unclear complex chemical system, optimization quality control markers been a long‐term challenge. In present study, feasible strategy integrated screening, determination, statistical analysis was efficiently proposed, especially for undefined hinese medicine injections. First, rapid separation LC...
Glioblastoma is the most common and lethal intracranial tumor type, characterized by high angiogenic infiltrative capacities. To provide a novel insight into therapeutic strategies against glioblastoma, cytotoxicity of arenobufagin hellebrigenin was investigated in human glioblastoma cell line, U-87. Similar dose-dependent observed cells, whereas no detectable toxicity confirmed mouse primary astrocytes. Treatment with each drug downregulated expression levels Cdc25C, Cyclin B1 survivin,...
Rationale Limonoids, characterized by a triterpenoid skeleton with furan ring, are unique secondary metabolites widely distributed in the families of Rutaceae , particularly Citrus species and Meliaceae. Studies on health benefits have demonstrated that limonoids range biological activities. Dietary intake citrus may provide protective effect against onset various cancers other xenobiotic related diseases. However, few studies about metabolic profiles been carried out. Methods The objectives...
RATIONALE Limonin and obacunone are two major limonoids distributed in the Rutaceae Meliaceae families. Their defined anti‐tumor activity is closely connected with furan ring multi‐carbonyls their structures. In vivo vitro biotransformations may influence structures further change effects. The metabolic profiles of limonin have not been studied previously. order to clarify metabolism, a comparative investigation pathways five different species liver microsomes zebrafish was carried out....
1. Huang–Lian–Jie–Du Decoction (HLJDD) is widely used for the treatment of hypertension, diabetes, inflammation and neural system diseases in clinic. In present study, comprehensive metabolic profile HLJDD was demonstrated reliably rapidly followed by pathway analysis six typical pure compounds (four alkaloids, one flavonoid iridoid) using LC–IT-MS combined with high resolution LC–FT-ICR-MS.2. Totally, 85 compounds, including 32 prototype components 53 biotransformed metabolites were...
Huang-Lian-Jie-Du Decoction (HLJDD) is a classical Traditional Chinese Medicine (TCM) formula with heat-dissipating and detoxifying effects. It used to treat inflammation-associated diseases. However, no systematic pharmacokinetic (PK) pharmacodynamic (PD) data concerning the activity of HLJDD under inflammatory conditions available date. In present study, concentration-time profiles hepatic clearance rates (HCR) 41 major components in rat plasma response oral administration clinical dose...
Nonalcoholic fatty liver disease (NAFLD) is the most common chronic hepatic characterized by excessive fat accumulation, and major risk for cardiovascular metabolic disorders. We explored therapeutic effect of gypenosides (Gyps) in combination with capsaicinoids (Caps) against high-fat-diet (HFD)-induced rat hyperlipidemia oxidized low-density lipoprotein (ox-LDL)-treated Raw264.7 cells. Network pharmacology analysis predicted that potential molecular mechanism Gyps/Caps was mainly...
Dihuang Baoyuan Granules is a prescription endorsed by HU Tianbao, renowned and elderly Chinese medicine practitioner from Beijing, has demonstrated definite clinical efficacy. The composition of this intricate as it includes 7 distinct herbal medicines. This study aims to analyze the chemical Granules, evaluate its efficacy in treatment diabetes distribution drug components plasma, liver, kidney after administration. findings will serve reference for future research on pharmacodynamic...
Objective: This study aims to elucidate and quantify the composition of Jiangtang Qingre formula (JQF), delineate absorbed components in bloodstream, predict major biologically active components, identify potential targets for treatment diabetes mellitus (DM). Materials Methods: The chemical metabolites JQF were elucidated using ultra-high-performance liquid chromatography (UHPLC)-linear ion trap quadrupole-orbitrap high-resolution mass spectrometry (MS). various concurrently determined...
A rapid and validated method was established for the simultaneous determination of eight active toxic bufadienolides in cinobufacini injection using high performance liquid chromatography coupled with triple quadrupole mass spectrometry. These compounds were separated within 3 min on a C 18 analytical column gradient elution. Eleven batches analyzed good linear regression relationship ( r , 0.9979–0.9999), precisions RSD 1.92–4.79%), repeatability 3.12–4.96%), stability 2.84–4.45%), recovery...
Alzheimer's disease (AD) is a progressive neurodegenerative caused by the complex interaction of multiple mechanisms. Recent studies examining effect high-fat diet (HFD) on AD phenotype have demonstrated significant influence both inflammation and cognition. However, different pathology reported conflicting conclusions. To explore involvement HFD in AD, we investigated phenotypic metabolic changes an mouse model response to HFD. The results indicated there was no Aβ levels or contextual...