Dengue shock syndrome is characterized by severe vascular leakage and disordered hemostasis progresses to death in 1 5 percent of cases. Although volume replacement recognized as the critical therapeutic intervention, World Health Organization management guidelines remain empirical rather than evidence-based.

Dengue is a public health problem in many countries. Rapid diagnosis of dengue can assist patient triage and management. Detection the viral protein, NS1, represents new approach to diagnosis.The sensitivity specificity Platelia NS1 ELISA assay an lateral flow rapid test (LFRT) were compared against gold standard reference diagnostic algorithm 138 Vietnamese children adults. Overall, was modestly more sensitive (82%) than LFRT (72%) confirmed cases. Both assays for primary secondary dengue,...

The pathophysiological basis of hemorrhage in dengue infections remains poorly understood, despite the increasing global importance these infections. A large prospective study 167 Vietnamese children with shock syndrome documented only minor prolongations prothrombin and partial thromboplastin times but moderate to severe depression plasma fibrinogen concentrations. detailed 48 revealed low concentrations anticoagulant proteins C, S, antithrombin III, which decreased severity shock, probably...

Japanese encephalitis (JE) causes at least 10 000 deaths each year. Death is presumed to result from infection, dysfunction and destruction of neurons. There no antiviral treatment. Seizures raised intracranial pressure (ICP) are potentially treatable complications, but their importance in the pathophysiology JE unknown. Between 1994 1997 we prospectively studied patients with suspected CNS infections referred an infectious disease referral hospital Ho Chi Minh City, Vietnam. We diagnosed...

Dengue hemorrhagic fever and dengue shock syndrome (DSS) are major causes of childhood morbidity mortality in many tropical countries. Increased intravascular permeability leading to is the cardinal feature DSS. Fluid resuscitation counteract massive plasma leakage mainstay treatment. A double-blind, randomized trial comparing four intravenous-fluid regimens for acute 50 children with DSS was conducted. Colloids (dextran or protein digest gelafundin 35,000) restored cardiac index blood...

Japanese encephalitis virus (JEV), the mosquito-borne flavivirus, annually causes an estimated 35,000-50,000 cases and 10,000-15,000 deaths in Asia, there is no antiviral treatment. The role played by immune response determining outcome of human infection with JEV poorly understood, although, animal models flavivirus encephalitis, unregulated proinflammatory cytokine responses can be detrimental.We studied innate, cellular, humoral 118 patients infected JEV, whom 13 (11%) died.Levels...

This paper reports on a differential pressure sensor using piezoresistive cantilever with the dimensions of 125 µm×100 µm×0.3 µm. The has higher sensitivity than traditional diaphragm because three free edges cantilever. measured results indicated that fabricated bent according to theory when was applied. and resolution were 3.2×10−4 Pa−1 0.02 Pa from −20 20 Pa, respectively.

Background. Dengue shock syndrome (DSS) is a severe manifestation of dengue virus infection that particularly affects children and young adults. Despite its increasing global importance, there are no prospective studies describing the clinical characteristics, management, or outcomes DSS.

Dengue virus infection is an increasingly important tropical disease, causing 100 million cases each year. Symptoms range from mild febrile illness to severe hemorrhagic fever. The pathogenesis incompletely understood, but immunopathology thought play a part, with antibody-dependent enhancement and massive immune activation of T cells monocytes/macrophages leading disproportionate production proinflammatory cytokines. We sought investigate whether defective population regulatory (T reg...

Background Dengue is the most important mosquito-borne viral infection to affect humans. Although it usually manifests as a self-limited febrile illness, complications may occur fever subsides. A systemic vascular leak syndrome that sometimes progresses life-threatening hypovolaemic shock serious complication seen in children, typically accompanied by haemoconcentration and thrombocytopenia. Robust evidence on risk factors, especially features present early illness course, for progression...

A rapid (<7-min) immunochromatographic test for immunoglobulin M (IgM) and IgG antibodies to dengue viruses was evaluated by using hospital admission discharge sera from 124 patients. The reference laboratory diagnosis based on the results of virus isolation, hemagglutination-inhibition assay (HAI), enzyme immunoassay (EIA). By standard assays, patients experienced primary infection (n = 30), secondary 48), Japanese encephalitis (JE) 20), or no flavivirus 26). demonstrated 100% sensitivity...

he mechanism underlying the transient vascular leak syndrome of dengue hemorrhagic fever (DHF) is unknown. We aimed to determine whether molecular size and charge selectivity, which help restrict plasma proteins within intravascular space, are altered in patients with DHF a disturbance anionic glycosaminoglycan (GAG) layer on luminal endothelial surface contributes disease pathogenesis.We measured serial levels fractional clearances different characteristics 48 children shock (DSS) urinary...

ABSTRACT Japanese encephalitis (JE) occurs in rural settings southern and eastern Asia, where diagnostic facilities are limited. For the diagnosis of JE virus (JEV) infection, we developed a nitrocellulose membrane-based immunoglobulin M (IgM) capture dot enzyme immunoassay (MAC DOT) that is rapid, simple to use, requires no specialized equipment, can distinguish JEV from dengue infection. In prospective field study Vietnam, 155 cerebrospinal fluid (CSF) 341 serum samples were collected 111...

The mechanisms underlying the bleeding manifestations and coagulopathy associated with dengue remain unclear, in part because of focus much previous work on severe disease without an appropriate comparison group. We describe detailed clinical laboratory profiles for a large group children all severities, similar non-dengue febrile illnesses, followed prospectively from early presentation through to recovery. Among dengue-infected patients but not controls, thrombocytopenia, increased partial...

DNA microarrays and specific reverse-transcription polymerase chain reaction assays were used to reveal transcriptional patterns in the blood of children presenting with dengue shock syndrome (DSS) well-matched patients uncomplicated dengue. The transcriptome acute was characterized by a metabolically demanding "host-defense" profile; transcripts related oxidative metabolism, interferon signaling, protein ubiquination, apoptosis, cytokines prominent. In contrast, DSS surprisingly benign,...

Dengue shock syndrome (DSS) is a potentially lethal complication of dengue virus infection associated with hypotension and leakage plasma water into the extravascular space. To determine whether underlying pathophysiology DSS distinct from that in milder forms disease, we assessed microvascular permeability, by use strain gauge plethysmography, Vietnamese children (n=19), or hemorrhagic fever (DHF) without (n=16), healthy control (n=15). At admission after fluid resuscitation, mean...

Background. Despite the availability of antitoxin and antibiotics, mortality rate for diphtheria remains high, mostly because cardiac complications. Methods. During 1 year, 154 Vietnamese children with admitted to a referral hospital were studied prospectively clinical examination, including simple pseudomembrane score, 12-lead 24-hour electrocardiography, measurement serum enzyme levels, estimation troponin T levels. Results. Thirteen had diphtheritic cardiomyopathy on admission, 19...

Purpose To identify risk factors and develop a prediction model for the development of profound recurrent shock amongst children presenting with dengue syndrome (DSS) Methods We analyzed data from prospective cohort DSS recruited at Paediatric Intensive Care Unit Hospital Tropical Disease in Ho Chi Minh City, Vietnam. The primary endpoint was “profound DSS”, defined as ≥2 episodes (for subjects compensated shock), or ≥1 initially decompensated/hypotensive and/or requirement inotropic...

ABSTRACT The performances of the MRL dengue fever virus immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA) and PanBio Dengue Duo IgM IgG ELISA were compared. Eighty sera from patients with infections, 24 Japanese encephalitis (JE), 78 nonflavivirus such as malaria, typhoid, leptospirosis, scrub typhus, used. test showed superior sensitivity for infections (94 versus 89%), while specificity JE (79 25%) other (100 91%). better overall performance, assessed by sum ( F...