A5078805860- Hemoglobin structure and function
- Heme Oxygenase-1 and Carbon Monoxide
- Erythrocyte Function and Pathophysiology
- Neonatal Health and Biochemistry
- Neuroscience of respiration and sleep
- Cardiac Arrest and Resuscitation
- Hemoglobinopathies and Related Disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- Blood transfusion and management
- Cancer, Hypoxia, and Metabolism
- High Altitude and Hypoxia
- Blood properties and coagulation
- Retinal Development and Disorders
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Neonatal Respiratory Health Research
- Neural dynamics and brain function
- Cardiac electrophysiology and arrhythmias
- Nanopore and Nanochannel Transport Studies
- Thermal Regulation in Medicine
- Respiratory Support and Mechanisms
- Asthma and respiratory diseases
- Dermatologic Treatments and Research
- Nitric Oxide and Endothelin Effects
Nara Medical University
2016-2025
Nara Medical University Hospital
2020-2023
St. Luke's International Hospital
2021
Juntendo University Shizuoka Hospital
2020
Waseda Bioscience Research Institute in Singapore
2010-2019
Saitama Children's Medical Center
2001-2017
Chiba University
2017
Food and Drug Safety Center
2017
Nara Prefectural University
2017
Chiba Rosai Hospital
2017
The effect of molecular dimension hemoglobin (Hb)-based O 2 carriers on the diameter resistance arteries ( A 0 , 158 ± 21 μm) and arterial blood pressure were studied in conscious hamster dorsal skinfold model. Cross-linked Hb (XLHb), polyethylene glycol (PEG)-conjugated Hb, hydroxyethylstarch-conjugated XLHb, polymerized PEG-modified vesicles (PEG-HbV) synthesized. Their diameters 7, 22, 47, 68, 224 nm, respectively. bolus infusion 7 ml/kg XLHb (5 g/dl) caused an immediate hypertension (+34...
Effect of increasing blood viscosity during extreme hemodilution on capillary perfusion and tissue oxygenation was investigated in the awake hamster skinfold model. Two isovolemic steps were performed with 6% Dextran 70 [molecular weight (MW) = 70,000] until systemic hematocrit (Hct) reduced by 65%. A third step Hct 75% same solution [low (LV)] or a high-molecular-weight 500 [MW 500,000, high (HV)]. Final plasma viscosities 1.4 2.2 cP (baseline 1.2 cP). to 11.2 ± 1.1% from 46.2 1.5% for LV...
A critical issue for clinical utilization of human ES cells (hESCs) is whether they can generate terminally mature progenies with normal function. We recently developed a method efficient production hematopoietic progenitors from hESCs by coculture murine fetal liver-derived stromal cells. Large numbers hESCs-derived erythroid generated the enabled us to analyze development erythropoiesis at clone level and investigate their The results showed that globin expression in individual clones...
mAb to murine C receptor type 1 (CR1) were produced and three of them characterized. One antibody, designated as 8C12, immunoprecipitated a protein 190,000 Mr from detergent extract surface-labeled spleen cells stained B but not T lymphocytes in fluorescent flow cytometry. It inhibited both CR1-mediated rosette formation the cofactor activity CR1 for factor I-mediated cleavage C3b, suggesting that it recognizes ligand-binding site CR1. The two other antibodies, 7G6 7E9, recognized different...
Elucidating the in vitro differentiation of human embryonic stem (ES) and induced pluripotent (iPS) cells is important for understanding both normal pathological hematopoietic development vivo. For this purpose, a robust simple system that can faithfully trace vivo hematopoiesis necessary. In study, we established novel serum-free monolayer culture pathway from ES/iPS to functional definitive blood via mesodermal progenitors. Stepwise tuning exogenous cytokine cocktails progenitors primitive...
beta1,4-Galactosyltransferase (UDP galactose: beta-N-acetylglucosaminide: beta1,4-galactosyltransferase; EC 2.4.1. 22) catalyzes the transfer of galactose from UDP-Gal to N-acetylglucosamine in penultimate stages terminal glycosylation N-linked complex oligosaccharides mammalian cells. Tobacco BY2 cells lack this Golgi enzyme. To determine what extent production a glycosyltransferase can alter pathway plant cells, tobacco suspension-cultured were stably transformed with full-length human...
Poly(ethylene glycol) (PEG5000)-conjugated phosphatidylethanolamine was introduced onto the surface of hemoglobin vesicles (HbV); phospholipid encapsulating concentrated Hb (d = 0.257 ± 0.087 μm; P50 32 Torr). The obtained PEG-modified HbV (HbV-PEG) studied for use as a red cell substitute from viewpoint rheology, properties, and hemodynamics. viscosity unmodified suspended in saline ([Hb] 10 g/dL) 2.6 cP (shear rate 358 s-1, 37 °C), less than that human blood (4 cP). However, when 5 g/dL...
Hemoglobin vesicles (Hb-V) are artificial red blood cells encapsulating highly concentrated hemoglobin (Hb) in liposomes comprising phospholipids, cholesterol, negatively charged lipids, and polyethylene glycol (PEG)-conjugated phospholipids. Safety efficacy of Hb-V as a transfusion alternative have been extensively studied. For this study, we prepared using the kneading method with rotation–revolution mixer an to conventional extrusion method. We optimized operation parameters obtain high...
The stability of hemoglobin vesicles (HbV) as an oxygen infusion was tested during the storage for 1 year at 4, 23, and 40 °C. surface HbV modified with poly(ethylene glycol) (PEG), suspension deoxygenated nitrogen bubbling. samples stored 4 23 °C showed a stable dispersion state year, though sample precipitation decomposition vesicular components, decrease in pH, 4% leakage total Hb after year. PEG chains on stabilize prevent aggregation fusion due to their steric hindrance. original metHb...
Hemoglobin-vesicle (HbV) has been developed to provide oxygen-carrying ability plasma expanders. Its restore the systemic condition after hemorrhagic shock was evaluated in anesthetized Wistar rats for 6 hrs resuscitation. The HbV suspended 5 g/dL recombinant human serum albumin (HbV/rHSA) at an Hb concentration of 8.6 g/dL.Prospective, randomized, controlled trial.Department Surgery, School Medicine, Keio University.Forty male rats.The were with 1.5% sevoflurane inhalation throughout...
Hemoglobin vesicles (HbV) as red cell substitutes were prepared from a purified carbonylhemoglobin (HbCO) solution and lipid mixture composed of phospholipids, cholesterol, α-tocopherol. The diameter was controlled to 251 ± 87 nm using an extrusion method; the penetrated through membrane filters with regulated pore sizes. After ligand exchanging reaction (HbCO → HbO2), oxygen affinity (P50) HbV 32 Torr, which coencapsulation pyridoxal 5′-phosphate. rate metHb formation in nonenzymatically...
1. We analyzed the light-evoked responses of retinal neurons by means a white-noise technique. Horizontal and bipolar cells produced modulation response that was linearly related to mean luminance large field light. The first-order kernels were capable reproducing cells' with fair degree accuracy. amplitude as well waveform changed change in luminance. This is parametric form adaptation. As time constant much longer than (memory), assumed be at dynamic steady state given 2. With presence...
One physiological significance of the red blood cell (RBC) structure is that NO binding Hb retarded by encapsulation with membrane. To clarify mechanism, we analyzed Hb-vesicles (HbVs) different intracellular concentrations, [Hb](in), and particle sizes using stopped-flow spectrophotometry. The apparent rate constant, k(on)('(NO)), HbV at [Hb](in) = 1 g/dl was 2.6 x 10(7) m(-1) s(-1), which almost equal to k(on)((NO)) molecular Hb, indicating lipid membrane presents no obstacle for binding....
Carbon monoxide (CO) has attracted attention as a possible therapeutic agent for affecting anti-inflammatory and antioxidant activities. Previously, CO-bound hemoglobin vesicle (CO-HbV) was developed nanotechnology-based CO donor, its safety profile potential clinically applicable carrier of were examined in vitro vivo. In the present study, efficacy CO-HbV against severe acute pancreatitis with secondary distal organ-injured model mice that fed choline-deficient ethionine-supplemented diet....
Macrophages play a central role in various inflammatory disorders and are broadly divided into two subpopulations, M1 M2 macrophage. In the healing process acute disorders, shifting production of macrophages to is desirable, because secrete pro-inflammatory cytokines, whilst variety anti-inflammatory cytokines. Previous findings indicate that when treated with carbon monoxide (CO), secretion cytokine increased expression cytokines inhibited, indicating CO may have potential modulate toward...