A5103144326- Antioxidant Activity and Oxidative Stress
- Retinoids in leukemia and cellular processes
- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Photosynthetic Processes and Mechanisms
- Food Chemistry and Fat Analysis
- Natural product bioactivities and synthesis
- Protein Interaction Studies and Fluorescence Analysis
- Retinopathy of Prematurity Studies
- Retinal Imaging and Analysis
- Cholesterol and Lipid Metabolism
- Ubiquitin and proteasome pathways
- Microbial Metabolites in Food Biotechnology
- Animal Genetics and Reproduction
- Cancer, Hypoxia, and Metabolism
- Phytochemistry and Biological Activities
- Metal complexes synthesis and properties
- Drug Transport and Resistance Mechanisms
- Fish Biology and Ecology Studies
- Seaweed-derived Bioactive Compounds
- Ichthyology and Marine Biology
- Biochemical and biochemical processes
- Biotin and Related Studies
- Glutathione Transferases and Polymorphisms
- Spectroscopy Techniques in Biomedical and Chemical Research
University of Utah
2015-2024
Eye Institute of Utah
2012-2016
Eye Center
2014
Institute of Botany
2005
Chinese Academy of Sciences
2005
Advanced age-related macular degeneration (AMD) is the leading cause of late onset blindness. We present results a genome-wide association study 979 advanced AMD cases and 1,709 controls using Affymetrix 6.0 platform with replication in seven additional cohorts (totaling 5,789 unrelated 4,234 controls). also comprehensive analysis copy-number variations polymorphisms for AMD. Our discovery data implicated between variant hepatic lipase gene (LIPC) high-density lipoprotein cholesterol (HDL)...
Lutein, zeaxanthin, and their metabolites are the xanthophyll carotenoids that form macular pigment of human retina. Epidemiological evidence suggests high levels these in diet, serum, macula associated with a decreased risk age-related degeneration (AMD), AREDS2 study is prospectively testing this hypothesis. Understanding biochemical mechanisms underlying selective uptakes lutein zeaxanthin into may provide important insights physiology health disease. GSTP1 zeaxanthin-binding protein, but...
Significance Among mammals, only primates can accumulate high levels of xanthophyll carotenoids in their retinas. We discovered that, unlike most other the major cleavage enzyme β,β-carotene-9′,10′-dioxygenase (BCO2) is inactive humans, explaining unique accumulation lutein, zeaxanthin, and meso -zeaxanthin primate macula. confirmed this discovery by demonstrating that BCO2 knockout mice zeaxanthin
Significance We have determined the spatial distribution of macular pigment carotenoids lutein and zeaxanthin in human retina using confocal resonance Raman microscopy found that is highly concentrated fovea, while more diffusely spread across macula at a relatively lower concentration. Our results imply may play important role than health disease, they demonstrate elegant ability imaging to probe biochemistry structure most region retina.
Dietary intake of lutein and zeaxanthin appears to be advantageous for protecting human retinal macular tissues from degenerative disorders such as age-related degeneration. Selective concentration just two the many dietary carotenoids suggests that uptake transport these xanthophyll into foveal region are mediated by specific xanthophyll-binding proteins GSTP1 which has previously been identified zeaxanthin-binding protein primate macula. Here, a membrane-associated lutein-binding (HR-LBP)...
To describe different patterns of macular pigment (MP) seen in fluorescence lifetime imaging ophthalmoscopy (FLIO) and to analyze ex vivo characteristics carotenoids.A total 31 eyes young healthy subjects, 4 from patients with albinism, 36 telangiectasia type 2 (MacTel), 24 retinitis pigmentosa, 1 eye a hole were included this clinic-based, cross-sectional study. All subjects underwent Heidelberg Engineering FLIO MP measurements (dual-wavelength autofluorescence). Fundus autofluorescence...
A crystal structure of the lutein-binding domain human StARD3 (StAR-related lipid-transfer protein 3; also known as MLN64) has been refined to 1.74 Å resolution. previous same determined 2.2 resolution highlighted homology with StARD1 and shared cholesterol-binding character. since recognized a carotenoid-binding in primate retina, where its biochemical function binding lutein specificity appears be well suited recruit this photoprotective molecule. The current structures correspond closely...
Purpose: Premature infants at risk of retinopathy prematurity (ROP) miss placental transfer the carotenoids lutein (L) and zeaxanthin (Z) during third trimester. We previously demonstrated that prenatal L Z supplementation raised carotenoid levels in birth Lutein Zeaxanthin Pregnancy (L-ZIP) study (NCT03750968). Based on their antioxidant effects bioavailability, we hypothesized maternal with macular would reduce ROP. To test this hypothesis, utilized "macular pigment mice" genetically...
Lutein, a dietary carotenoid, selectively accumulates in human retina and brain. While many epidemiological studies show evidence of relationship between lutein status cognitive health, lutein's selective uptake brain tissue its potential function early neural development health have been poorly evaluated at molecular level. The objective this study was to evaluate the cross-sectional concentrations StARD3 (identified as binding protein retinal tissue) among three age groups: infants (1–4...
Significance Very-long-chain polyunsaturated fatty acids (VLC-PUFAs) longer than 26 carbons are not present in normal vertebrate diets, yet they uniquely found the retina and a few other tissues, implying physiological importance of their biosynthesis from shorter-chain dietary precursors via action ELOVL4 enzyme. While progress has been made by studying retinal samples human donors investigating mouse models Elovl4 dysfunction, further physiology interventional research hampered scarcity...
meso-Zeaxanthin is a carotenoid that rarely encountered in nature outside of the vertebrate eye. It not constituent normal human diet, yet this comprises one-third primate macular pigment. In current study, we undertook systematic approach to biochemically characterize production meso-zeaxanthin eye.Fertilized White Leghorn chicken eggs were analyzed for presence carotenoids during development. Yolk, liver, brain, serum, retina, and RPE/choroid isolated, extracted. The samples on C-30 or...
Flavonoids are common polyphenolic compounds widely distributed in fruits and vegetables. These pigments have important pharmacological relevance because emerging research suggests possible anti-cancer anti-inflammatory properties as well other beneficial health effects. relatively hydrophobic molecules, suggesting the role of blood transport proteins their delivery to tissues. In this study, we assess binding interactions four flavonoids (kaempferol, luteolin, quercetin, resveratrol) with...