A5053330204- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Microbial infections and disease research
- Myxozoan Parasites in Aquatic Species
- Identification and Quantification in Food
- Invertebrate Immune Response Mechanisms
- Aquaculture Nutrition and Growth
- Parasite Biology and Host Interactions
- Genetic and phenotypic traits in livestock
- Fish Biology and Ecology Studies
- Marine and environmental studies
- Animal health and immunology
- Genetic diversity and population structure
- Pharmaceutical and Antibiotic Environmental Impacts
- Reproductive Physiology in Livestock
- Mosquito-borne diseases and control
- RNA and protein synthesis mechanisms
- Bioactive Compounds and Antitumor Agents
- Nigella sativa pharmacological applications
- Actinomycetales infections and treatment
- Nutritional Studies and Diet
- Diphtheria, Corynebacterium, and Tetanus
- Infectious Diseases and Mycology
- Nanoparticles: synthesis and applications
- Consumer Attitudes and Food Labeling
Texas A&M University
2023-2025
Assiut University
2010-2024
Auburn University
2012-2023
University of Wisconsin–Milwaukee
2020-2023
Mohammed V University
2021
University of Madras
2009
Journal Article Characterization of the gut microbiota three commercially valuable warmwater fish species Get access A.M. Larsen, Larsen Aquatic Microbiology Laboratory School Fisheries, Aquaculture, and Sciences Center for Advanced Science, Innovation, Commerce Auburn University AL USA Search other works by this author on: Oxford Academic Google Scholar H.H. Mohammed, Mohammed C.R. Arias Correspondence Cova R. Arias, Laboratory, Sciences, Commerce, University, 559 Devall Drive, Auburn,...
Abstract Aquaculture is now the main source of seafood in human diets and one its fastest‐growing industries worldwide. However, industry facing several difficulties, including infectious diseases, most significant limiting factor for aquaculture expansion. The impact diseases on growth, fecundity, mortality rates, marketability profound. Hence, ability to predict disease outbreaks crucial overcoming these challenges. Various agents such as bacteria, viruses, fungi, parasites can cause...
The external microbiome of fish is thought to benefit the host by hindering invasion opportunistic pathogens and/or stimulating immune system. Disruption those microbial communities could increase susceptibility diseases. Traditional aquaculture practices include use potent surface-acting disinfectants such as potassium permanganate (PP, KMnO4) treat infections. This study evaluated effect PP on channel catfish and investigated if dysbiosis leads an in disease susceptibility. Columnaris...
Flavobacterium psychrophilum causes bacterial cold-water disease in wild and aquaculture-reared fish is a major problem for salmonid aquaculture. The mechanisms responsible are not known. It was recently demonstrated that the related pathogen, columnare, requires functional type IX protein secretion system (T9SS) to cause disease. T9SSs secrete cell surface adhesins, gliding motility proteins, peptidases, other enzymes, any of which may be virulence factors. F. genome has genes predicted...
The first objective of the study aimed to detect presence Lactococcus petauri, L. garvieae, and formosensis in fish (n = 359) environmental 161) samples from four lakes near an affected farm California during outbreak 2020. second was compare virulence spp. Rainbow Trout Oncorhynchus mykiss Largemouth Bass Micropterus salmoides. Standard bacterial culture methods were used isolate brain posterior kidney sampled lakes. Quantitative PCR (qPCR) utilized DNA tissues Laboratory controlled...
Abstract This study reports on an outbreak of motile Aeromonas septicemia (MAS) that caused significant mortalities in iridescent shark catfish ( Pangasius hypophthalmus ) biofloc systems the Punjab province Pakistan. Clinical symptoms observed affected fish included anal fin hemorrhaging, mouth area bleeding, skin ulcerations, interstitial nephritis, and focal fusion secondary lamellae. According to necropsy findings a molecular phylogenetic analysis based gyrB gene sequences, etiologic...
This study investigated the impacts of bacterial DNA extraction methodology on downstream analysis fish gut microbiota. Feces and intestine samples were taken from three sympatric freshwater species with varying diets. Samples processed immediately (approximately 4 h after capture; fresh), stored at −20°C for 15 days or preserved in RNAlater® reagent days. was then extracted using two commercial kits: one designed animal tissues specifically formulated stool samples. Microbial community...
Piscine lactococcosis is an emergent bacterial disease that associated with high economic losses in many farmed and wild aquatic species worldwide. Early accurate detection of the causative agent piscine essential for management fish farms. In this study, a TaqMan quantitative polymerase chain reaction (qPCR) targeting 16S-23S rRNA internal transcribed spacer region was developed validated. Validation qPCR performed DNA previously typed L. petauri garvieae recovered from different hosts...
In the southeastern USA, columnaris disease (caused by Flavobacterium columnare) typically affects catfish raised in earthen ponds from early spring until late summer. Recently, unusually severe outbreaks of occurred at E. W. Shell Fisheries Center located Auburn, AL, USA. During these outbreaks, and other aquaculture sport fish species that were within same watershed affected. Our objective was to investigate genetic diversity among F. columnare isolates recovered different sites, sources,...
Flavobacterium columnare causes columnaris disease in freshwater fish both natural and aquaculture settings. This is often lethal, especially when population density high, control options such as vaccines are limited. The type IX secretion system (T9SS) required for F. virulence, but secreted virulence factors have not been fully identified. Many T9SS-secreted proteins predicted peptidases, peptidases common of other pathogens. T9SS-deficient mutants, ΔgldN ΔporV, exhibit strong defects...
Abstract Unusual persistent natural mortality occurred in a floating in‐pond raceway system intensively stocked with channel and hybrid catfish beginning early November 2016 up until March 2017. The temperature during the period of outbreak ranged from 7.2 to 23.7°C. Gross examination freshly dead moribund fish revealed pale gills, slight abdominal distension swollen inflamed vents. Comprehensive necropsy 20 demonstrated vast amounts bloody ascitic fluid coelomic cavity, visceral congestion,...
Flavobacterium columnare causes columnaris disease in wild and cultured freshwater fish is a major problem for sustainable aquaculture worldwide. The F. type IX secretion system (T9SS) secretes many proteins required virulence. T9SS component GldN gliding motility over surfaces. Genetic manipulation of inefficient, which has impeded identification secreted that are critical Here, we identified virulent wild-type strain (MS-FC-4) highly amenable to genetic manipulation. This facilitated...
Columnaris disease, caused by the bacterium Flavobacterium columnare, is currently most frequently reported bacterial disease affecting farm-raised channel catfish in USA. Common treatments against include use of medicated feed that has led to emergent antibiotic resistant strains F. columnare. Nigella sativa (Black cumin) a medicinal herb commonly used many cultures as natural remedy for numerous disorders. Recently, we have discovered antibacterial activity N. and its oil extract In this...
Abstract Background The equine conjunctival microbiota has often been reported to be dominated by Gram‐positive species such as Staphylococcus sp. , Bacillus sp., and Corynebacterium However, traditional culture‐based methods can only recover a fraction of the bacterial present in sample. Objectives This pilot study aimed at exploring diversity using culture‐independent methods. Study design Eight horses were included this study, eyes with normal ophthalmic examination (n = 15 eyes) sampled....
White bass (Morone chrysops), striped and their interspecific hybrid are important game fishes, whereas the is an aquaculture species in US. Numerous state, federal private hatcheries, therefore, rear these for stocking purposes as well food fish. Although populations (both wild domesticated) have been extensively evaluated, relatively little effort has directed toward study improvement of white bass. In this study, we developed SNP resources to examine genetic relationships among a...